Propidium iodide CAS 25535-16-4

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Physical properties

Molecular weight	668.39
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	6000¹
Excitation (nm)	537
Emission (nm)	618
Quantum yield	0.2¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Alternative formats

Propidium iodide *10 mM aqueous solution*

Propidium iodide *1 mg/mL aqueous solution*

Overview SDS Protocol

CAS

25535-16-4

Molecular weight

668.39

Extinction coefficient (cm ^-1 M ^-1)

6000¹

Excitation (nm)

537

Emission (nm)

618

Quantum yield

0.2¹

Propidium iodide (PI) belongs to the same chemical class of ethidium bromide. As in the case of ethidium bromide its fluorescence is enhanced for 20-30-fold upon binding to nucleic acids. The fluorescence excitation maximum is red-shifted for 30-40 nm and the fluorescence emission maximum blue-shifted for 15 nm or so. PI also binds to RNA as DAPI and acridine orange. PI is commonly used for identifying dead cells in a population of cells and as a counterstain in multicolor fluorescent techniques. It can also be used to differentiate necrotic, apoptotic and normal cells. It is suitable for fluorescence microscopy, flow cytometry and fluorometry.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Propidium iodide *CAS 25535-16-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	149.613 µL	748.066 µL	1.496 mL	7.481 mL	14.961 mL
5 mM	29.923 µL	149.613 µL	299.226 µL	1.496 mL	2.992 mL
10 mM	14.961 µL	74.807 µL	149.613 µL	748.066 µL	1.496 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	6000¹
Excitation (nm)	537
Emission (nm)	618
Quantum yield	0.2¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
DiR iodide [1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide]	754	778	270000¹
DiI iodide [1,1-Dioctadecyl-3,3,3,3- tetramethylindocarbocyanine iodide]	550	564	148000

Images

Figure 1. Chemical structure for Propidium iodide *CAS 25535-16-4*

Citations

View all 16 citations: Citation Explorer

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Chlorite and bromate alter the conjugative transfer of antibiotic resistance genes: Co-regulation of oxidative stress and energy supply
Authors: Cao, Jinrui and Xue, Bin and Yang, Shuran and Yang, Xiaobo and Zhang, Xi and Qiu, Zhigang and Shen, Zhiqiang and Wang, Jingfeng
Journal: Journal of Hazardous Materials (2024): 134257

Cylindrospermopsin enhances the conjugative transfer of plasmid-mediated multi-antibiotic resistance genes through glutathione biosynthesis inhibition
Authors: Yang, Shuran and Cao, Jinrui and Zhao, Chen and Zhang, Xi and Li, Chenyu and Wang, Shang and Yang, Xiaobo and Qiu, Zhigang and Li, Chao and Wang, Jingfeng and others,
Journal: Ecotoxicology and Environmental Safety (2024): 116288

Development and characterization of biomimetic peptide-based bioink for dental tissue engineering
Authors: Altan, Zeynep
Journal: (2023)

Microfluidic System for Cell Mixing and Particle Focusing Using Dean Flow Fractionation
Authors: Wiede, Alexander and Stranik, Ondrej and Tannert, Astrid and Neugebauer, Ute
Journal: (2023): 671--685

The role of non-coding RNA ASBEL regulation of BTG3 in pancreatic ductal adenocarcinoma tumor progression
Authors: Chen, Jing and Zhu, Ming-Yuan and Huang, Yan-Hua and Ling, Yi-Ting and Gu, Tian-Yuan and Zhou, Quan and Fei, Ming-Jian and Zhou, Zhong-Cheng
Journal: Current Therapeutic Research (2023): 100700

Protective role of melatonin against testicular damage caused by polymicrobial sepsis in adult rats
Authors: Doganay, Songul and Budak, Ozcan and Toprak, Veysel and Erman, Gulay and Sahin, Arzu
Journal: Ulus Travma Acil Cerrahi Derg (2022): 723--729

Detection and differentiation of bacterial and fungal infection of neutrophils from peripheral blood using Raman spectroscopy
Authors: Arend, Natalie and Pittner, Angelina and Ramoji, Anuradha and Mondol, Abdullah S and Dahms, Marcel and R{\"u}ger, Jan and Kurzai, Oliver and Schie, Iwan W and Bauer, Michael and Popp, J{\"u}rgen and others,
Journal: Analytical Chemistry (2020)

Development and Characterization of Electrospun Polymer Scaffolds for 3D Cell Culture Model to Mimic Human Intestine System
Authors: Firik, Tu{\u{g}}ba
Journal: (2020)

Biomimetic hybrid scaffold consisting of co-electrospun collagen and PLLCL for 3D cell culture
Authors: Türker, Esra and Yildiz, Umit Hakan and Yildiz, Ahu Arslan
Journal: International journal of biological macromolecules (2019)

References

View all 60 references: Citation Explorer

Analysis of cycloheximide-induced apoptosis in human leukocytes: Fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide
Authors: Baskic D, Popovic S, Ristic P, Arsenijevic NN.
Journal: Cell Biol Int (2006): 924

Prognostic significance of plasma cell propidium iodide and annexin-V indices and their mutual ratio in multiple myeloma
Authors: Scudla V, Ordeltova M, Minarik J, Dusek L, Zemanova M, Bacovsky J.
Journal: Neoplasma (2006): 213

Assessment of the cell viability of cultured Perkinsus marinus (Perkinsea), a parasitic protozoan of the Eastern oyster, Crassostrea virginica, using SYBRgreen-propidium iodide double staining and flow cytometry
Authors: Soudant P, Chu FL, Lund ED.
Journal: J Eukaryot Microbiol (2005): 492

Single UV excitation of Hoechst 33342 and propidium iodide for viability assessment of rhesus monkey spermatozoa using flow cytometry
Authors: Cai K, Yang J, Guan M, Ji W, Li Y, Rens W.
Journal: Arch Androl (2005): 371

The assessment of viability in isolated rat hepatocytes subjected to cold or subzero non-freezing preservation protocols using a propidium iodide modified test
Authors: Giraudi PJ, Almada LL, Mamprin ME, Guibert EE, Furno G, Rodriguez JV.
Journal: Cryo Letters (2005): 169

Propidium iodide and PicoGreen as dyes for the DNA fluorescence correlation spectroscopy measurements
Authors: Kral T, Widerak K, Langner M, Hof M.
Journal: J Fluoresc (2005): 179

Low propidium iodide intensity in flow cytometric white blood cell counting as a marker of cell destruction
Authors: Wagner T, Guber SE, Stubenrauch ML, Lanzer G, Neumueller J.
Journal: Transfusion (2005): 228

Evaluation of plasma cell propidium-iodide and annexin-V indices: Their relation to prognosis in multiple myeloma
Authors: Minarik J, Scudla V, Ordeltova M, Bacovsky J, Zemanova M.
Journal: Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub (2005): 271

Green fluorescent protein-propidium iodide (GFP-PI) based assay for flow cytometric measurement of bacterial viability
Authors: Lehtinen J, Nuutila J, Lilius EM.
Journal: Cytometry A (2004): 165

Evaluation of cytotoxic function and apoptosis in interleukin (IL)-12/IL-15-treated umbilical cord or adult peripheral blood natural killer cells by a propidium-iodide based flow cytometry
Authors: Lin SJ, Cheng PJ, Huang YJ, Kuo ML.
Journal: Pediatr Allergy Immunol (2004): 79