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Antibody Labeling

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ReadiLink™ Rapid Antibody Labeling Kits from AAT Bioquest provide a convenient method for labeling microscale volumes of antibody (50 or 100 µg) with our superior iFluor™ and mFluor™ dyes, as well as other small organic dyes. Choose from over 20 fluorescent labels for use in fluorescence-based imaging or flow cytometry. Also available are ReadiLink™ Rapid Antibody Labeling kits for conjugation of biotin and hapten-carriers, BSA and KLH, to biomolecules for use in immunization and antibody production.
 
 
Antibody conjugation with ReadiLink™ Rapid Antibody Labeling Kits
Ordering information for ReadiLink™ Rapid Antibody Labeling Kits
 
 
 

 
ReadiLink™ Rapid Antibody Labeling Kit selection guide
 
 Fluorophore Fluorophore Equivalent Ex/Em (nm) Imaging Flow Cytometry Cat No.
 iFluor™ 350 AMCA, Alexa Fluor® 350 345/442   1220
 iFluor™ 488 FITC, Alexa Fluor®  488 491/514 1255
 iFluor™ 555 Cy3, Alexa Fluor® 555  552/567   1227
 iFluor™ 594  Texas Red®, Alexa Fluor® 594 592/614   1230
 iFluor™ 633 Cy5, Alexa Fluor® 633  638/655 1260
 iFluor™ 647 Cy5, Alexa Fluor® 647  654/674 1235
 iFluor™ 680 Cy5.5, Alexa Fluor® 680, IRDye® 700  682/701 1240
 iFluor™ 700 Alexa Fluor® 700  693/713 1245
 iFluor™ 750 Cy7, Alexa Fluor® 750  753/779   1250
 iFluor™ 790 IRDye® 800, Alexa Fluor® 790  782/811   1265
 mFluor™ Violet 420 Cascade® Blue  398/411   1105
 mFluor™ Violet 450 Pacific Blue®  403/454   1100
 mFluor™ Violet 510   414/508   1110
 mFluor™ Violet 540 Pacific Orange®  405/537   1114
 mFluor™ Blue 570 R-PE  553/570   1120
 mFluor™ Green 620   522/617   1123
 mFluor™ Red 700 APC-Alexa Fluor® 700 Tandem  657/700   1130
 mFluor™ Red 780  APC-Alexa Fluor® 750 Tandem  629/780   1131
 FITC   494/520 1299
 Cy3   555/565   1290
 Cy5   644/665 1292
 Cy7   749/776   1294
 


How ReadiLink™ Rapid Antibody Labeling Kits work
 
ReadiLink™ Rapid Antibody Labeling Kits utilize fluorophores coupled to an amine-reactive moiety (succinimidyl ester). These reactive dyes selectivley bind to aliphatic amines of proteins forming carboxamide bonds. Once formed these carboxamide bonds are extremely stable, ensuring no dissociation between label and protein. After conjugation and the addition of a TQ™-Dyed quench buffer, any free unbound ReadiLink™ labels will bind with the quenchers and become non-fluorescent. Addition of the the TQ™-Dyed quench buffer is key as it eliminates background interference as well as the column purification step.
 
ReadiLink™ Antibody Labelings kits include all the essential components for performing two conjugation reactions (2 x 50 µg). Each kit can be used to label monoclonal or polyclonal antibodies or other proteins (>10 kD).
 
 
 
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ADDITIONAL RESOURCES

Product Ordering Information
 
Table 1. Ordering information for ReadiLink™ Rapid iFluor™ Antibody Labeling Kits.
Cat# Product Name Ex (nm) Em (nm) Size
1220 ReadiLink™ Rapid iFluor™ 350 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 345 442 2 Labelings
1255 ReadiLink™ Rapid iFluor™ 488 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 491 514 2 Labelings
1227 ReadiLink™ Rapid iFluor™ 555 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 552 567 2 Labelings
1230 ReadiLink™ Rapid iFluor™ 594 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 592 614 2 Labelings
1260 ReadiLink™ Rapid iFluor™ 633 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 638 655 2 Labelings
1235 ReadiLink™ Rapid iFluor™ 647 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 654 674 2 Labelings
1240 ReadiLink™ Rapid iFluor™ 680 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 682 701 2 Labelings
1245 ReadiLink™ Rapid iFluor™ 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 693 713 2 Labelings
1250 ReadiLink™ Rapid iFluor™ 750 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 753 779 2 Labelings
1265 ReadiLink™ Rapid iFluor™ 790 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 782 811 2 Labelings
 
Table 2. Ordering information for ReadiLink™ Rapid mFluor™ Antibody Labeling Kits.
Cat# Product Name Ex (nm) Em (nm) Size
1105 ReadiLink™ Rapid mFluor™ Violet 420 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 398 411 2 Labelings
1100 ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 403 454 2 Labelings
1110 ReadiLink™ Rapid mFluor™ Violet 510 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 414 508 2 Labelings
1114 ReadiLink™ Rapid mFluor™ Violet 540 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 405 537 2 Labelings
1120 ReadiLink™ Rapid mFluor™ Blue 570 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 553 570 2 Labelings
1123 ReadiLink™ Rapid mFluor™ Green 620 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 522 617 2 Labelings
1130 ReadiLink™ Rapid mFluor™ Red 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 657 700 2 Labelings
1131 ReadiLink™ Rapid mFluor™ Red 780 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 629 780 2 Labelings
 
Table 3. Ordering information for ReadiLink™ Rapid trFluor™ Antibody Labeling Kits.
Cat# Product Name Ex (nm) Em (nm) Size
1300 ReadiLink™ Rapid trFluor™ Eu Antibody Labeling Kit *Microscale Optimized for Labeling 50 ug Antibody Per Reaction* 346 617 2 Labelings
 
Table 4. Ordering information for ReadiLink™ Antibody Labeling Kits.
Cat# Product Name Ex (nm) Em (nm) Size
5501 ReadiLink™ BSA Conjugation Kit     1 kit
5502 ReadiLink™ KLH Conjugation Kit     1 kit
5520 ReadiLink™ Protein Biotinylation Kit     2 Labelings
5521 ReadiLink™ Protein Biotinylation Kit *Powered by ReadiView™ Biotin Visionization Technology*     1 kit
1299 ReadiLink™ Rapid FITC Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 492 516 2 Labelings
1290 ReadiLink™ Rapid Cy3 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 555 565 2 Labelings
1292 ReadiLink™ Rapid Cy5 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 649 665 2 Labelings
1294 ReadiLink™ Rapid Cy7 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 749 776 2 Labelings

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