Fluorescence imaging is a technique using instruments such as fluorescence microscopes to visualize fluorescent dyes or proteins tagged to targets of interest. It is a sensitive and quantitative method that is widely used in molecular biology, cell biology and biochemistry laboratories for a variety of experimental observations. Such observations include the location and dynamics of gene expression, protein expression and molecular interactions in cells and tissues. For subcellular imaging of organelles such as cell membranes, lysosome and mitochondria AAT Bioquest offers Cell Navigator™ fluorescence imaging kits. Each kit includes a set of robust fluorescence tools optimized for live cell imaging and analysis of cellular events in a spatial and temporal context.
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Fluorescence images of HeLa cells stained with Cell Navigator™ Mitochondrion Staining Kit *Green Fluorescence*. Live cells were stained with mitochondria dye MitoLite™ Green (Green) and imaged using fluorescence microscope with a FITC filter set. After fixation in 4% formaldehyde, the cells were labeled with F-actin dye iFluor™ 633-Phalloidin (Cat#23125, Red) and counterstained with Nuclear Blue™ DCS1 (Cat#17548, Blue).
Images of HeLa cells stained with A: Cell Navigator™ Lysosome Staining Kit (Cat# 22658), B: LysoTracker® Red DND-99 (from Invitrogen) in a Costar black wall/clear bottom 96-well plate. The signals were compared at 0 and 120 seconds exposure time by using an Olympus fluorescence microscope.
Fluorescence image of live HeLa cells stained with Nuclear Violet™ LCS1(Cat. 17543) and MitoLite™ Green FM (Cat. 22695) using fluorescence microscope with a DAPI and FITC filter set.
Fluorescence images of endoplasmic reticulum (ER) staining in HeLa cells cultured in a 96-well black-wall clear-bottom plate using fluorescence microscope with a FITC filter set. Left: Live cells were stained with ER-selective probe ER Green™ (Cat#22635, Green), mitochondria dye MitoLite™ Red FX600 (Cat#22677, Red) and nuclei stain Hoechst 33342 (Cat#17530, Blue). Right: Live cells stained with ER Green™ (Cat22635, Green) were fixed with 4% formaldehyde, and labeled with F-actin dye iFluor™ 594-Phalloidin (Cat#23122, Red) and nuclei stain DAPI (Cat#17507, Blue).
Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with Cell Navigator™ F-Actin Labeling Kit *Green Fluorescence* in a Costar black 96-well plate. Cell were labeled with iFluor™ 488-Phalloidin (Cat#22261, Green) and nuclei stain DAPI (Cat#17507, Blue), respectively. Cell endoplasmic reticulum (ER) was stained with ER Red™ (Cat#22636, Red) before fixation.
The fluorescence images of HeLa cells stained with Cellpaint™ Deep Red in a 96-well black wall/clear bottom plate. The cells were imaged using fluorescence microscope with a Cy5 filter.