AmpliteiFluorReadiUseCell MeterSignal Guard

Horseradish Peroxidase (HRP)

View all products
Related:
Horseradish peroxidase (HRP) is a popular enzyme used extensively in molecular biology for the detection of immune complexes and targets such as proteins, carbohydrates or nucleic acids. This enzyme, which is typically conjugated to antibodies, streptavidin or other proteins, functions as a reporter system for probe-based immunoassay applications including, immunohistochemistry (IHC), Western blot (WB), and enzyme-linked immunosorbent assays (ELISAs).
 
AAT Bioquest offers a complete portfolio of HRP labeling tools and services, HRP conjugates labeled to streptavidin, secondary antibodies and small organic molecules, as well as enzyme activity and quantitation assay kits. We also offer a wide selection of HRP substrates to satisfy any experimental design and instrument setup.
 
See all HRP related products
 
 

 
Why HRP as an enzyme reporter system
 
The widespread adoption of HRP as enzyme reporter system is primarily due to three factors. First, HRP has the ability to amplify weak signals and enhance detectability of low abundance target molecules. Second, the relatively small size of HRP (~44 kDa) compared to other reporter enzymes (e.g. alkaline phosphatase (~140 kDa)) further improves intracellular penetration into samples, reduces steric hindrance and minimizes immunoreactivity loss. Third, is the high turnover rate and good stability of HRP that enables rapid and strong signal generation. 
 
Visualizing HRP
 
By itself, the HRP enzyme, or conjugates thereof, have very little value. Its presence can only be visualized with the addition of a suitable substrate that, when oxidized by HRP using hydrogen peroxide, produces a quantifiable signal. A variety of HRP substrates have been developed and commercialized to exploit the desirable features of this enzyme. These substrates fall into three distinct categories: chromogenic, fluorogenic or chemiluminescent substrates. The choice of substrate depends on the type of instrument to be used for signal-detection, and should be optimized for the intended system and detection limit. 
 


HRP Labeling Tools & Services
 
ReadiUse™ Preactivated HRP reagents and Buccutite™ Peroxidase (HRP) Antibody Conjugation kits offer a convenient and robust process for conjugating antibodies to HRP. Featuring streamlined protocols, produce stable HRP-antibody conjugates in two simple mixing steps.

For custom conjugation of HRP to an antibody in our catalog or as a stand-alone service for customer-supplied materials, refer to our Bioconjugation Services.


 
HRP Conjugates
 
ReadiUse™ Preactivated HRP reagents and Buccutite™ Peroxidase (HRP) Antibody Conjugation kits offer a convenient and robust process for conjugating antibodies to HRP. Featuring streamlined protocols, produce stable HRP-antibody conjugates in two simple mixing steps.
 
For custom conjugation of HRP to an antibody in our catalog or as a stand-alone service for customer-supplied materials, refer to our Bioconjugation Services.

 
HRP Substrates
 
Choose form our large assortment of chromogenic, fluorogenic and luminescent HRP substrates available in a variety of sensitivities – compatible with any instrument setup. For detection of low-abundance targets in cells and tissues, use iFluor™ Styramide reagents compatible with ICC, IHC and FISH procedures. For assistance selecting a substrate, see tables below.

 
Amplite™ Assay Kits
 
Amplite™ enzyme-based assays provide a sensitive and simple method for the quantitation of a diverse array of analytes, including glucose, catalasexanthine, acetylcholine and choline, as well as, hydrogen peroxide.


ADDITIONAL RESOURCES

Table 1. Colorimetric substrate product guide for ELISA, Western Blot and IHC applications.

Cat# Product Name Abs (nm) Color Size Price
11001 ReadiUse™ ABTS Substrate Solution  420 nm Green  1 L $175
11003 ReadiUse™ TMB Substrate Solution  450 nm or 650 nm Yellow or Blue  1 L $495
11012 ReadiUse™ TMB Substrate Solution  450 nm or 650 nm Yellow or Blue  100 mL $75

 

Table 2. Fluorimetric substrate product guide for ELISA, Western Blot and IHC applications.

Cat# Product Name Ex (nm) Em (nm) Size Price
11000 Amplite™ ADHP  571 585 25 mg $195
11005 Amplite™ Blue 324 409 25 mg $145
11009 Amplite™ IR 647 670 1 mg $95
11011 Amplite™ Red 571 585 1000 Assays $95

 

Table 3. Luminescent substrate product guide.

Cat# Product Name Ex (nm) Em (nm) Size Price
11050 Luminol [3-Aminophthalhydrazide] *CAS 521-31-3* 355 411 1 g $75

 

Table 4. iFluor™ Power Styramide Amplification reagents and imaging kits.

Cat# Product Name Ex (nm) Em (nm) Size Price
45000 iFluor™ 350 Styramide *Superior Replacement for Alexa Fluor 350 tyramide* 345 442 100 Slides $295
45020 iFluor™ 488 Styramide *Superior Replacement for Alexa Fluor 488 tyramide* 491 514 100 Slides $295
45025 iFluor™ 546 Styramide *Superior Replacement for Alexa Fluor 546 tyramide* 541 557 100 Slides $295
45027 iFluor™ 555 Styramide *Superior Replacement for Alexa Fluor 555 tyramide* 552 567 100 Slides $295
45030 iFluor™ 568 Styramide *Superior Replacement for Alexa Fluor 568 tyramide* 568 587 100 Slides $295
45035 iFluor™ 594 Styramide *Superior Replacement for Alexa Fluor 594 tyramide* 592 619 100 Slides $295
45045 iFluor™ 647 Styramide *Superior Replacement for Alexa Fluor 647 tyramide* 649 665 100 Slides $295
45050 iFluor™ 680 Styramide *Superior Replacement for Alexa Fluor 680 tyramide* 676 695 100 Slides $295
45055 iFluor™ 700 Styramide *Superior Replacement for Alexa Fluor 700 tyramide* 685 710 100 Slides $295
45065 iFluor™ 750 Styramide *Superior Replacement for Alexa Fluor 750 tyramide* 749 775 100 Slides $295
45070 iFluor™ 790 Styramide *Superior Replacement for Alexa Fluor 790 tyramide* 782 811 100 Slides $295
45100 iFluor™ 488 tyramide 491 514 200 slides $295
45105 iFluor™ 555 Tyramide 552 567 200 Slides $295
45110 iFluor™ 647 Tyramide 649 665 200 Slides $295
45200 iFluor 350™ PSA™ Imaging Kit with Goat Anti-Rabbit IgG 345 442 100 Tests $495
45205 iFluor 488™ PSA™ Imaging Kit with Goat Anti-Rabbit IgG 491 514 100 Tests $495
45220 iFluor 555™ PSA™ Imaging Kit with Goat Anti-Rabbit IgG 552 567 100 Tests $495
45230 iFluor 594™ PSA™ Imaging Kit with Goat Anti-Rabbit IgG 592 619 100 Tests $495
45240 iFluor 647™ PSA™ Imaging Kit with Goat Anti-Rabbit IgG 649 665 100 Tests $495
45250 iFluor 350™ PSA™ Imaging Kit with Goat Anti-Mouse IgG 345 442 100 Tests $495
45260 iFluor 488™ PSA™ Imaging Kit with Goat Anti-Mouse IgG 491 514 100 Tests $495
45270 iFluor 555™ PSA™ Imaging Kit with Goat Anti-Mouse IgG 552 567 100 Tests $495
45280 iFluor 594™ PSA™ Imaging Kit with Goat Anti-Mouse IgG 592 619 100 Tests $495
45290 iFluor 647™ PSA™ Imaging Kit with Goat Anti-Mouse IgG 649 665 100 Tests $495
45300 Biotin Styramide *Superior Replacement for Biotin Tyramide*     100 Slides $395
45305 DIG Styramide *Superior Replacement for DIG tyramide*     100 Slides $395

View all products