Viability and Proliferation
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Cell proliferation is an increase in the number of cells as a direct result of growth and division. It is regulated by the cell cycle, which is divided into four distinct phases: G1 phase, S phase (synthesis), G2 phase and M phase (mitosis). Assays investigating cell proliferation are used to monitor the growth rate of a cell population. Cell viability assays are excellent tools for validating the effectiveness of novel pharmacological compounds by measuring the percentage of remaining viable cells in a sample population after drug exposure. Although cell proliferation, viability and apoptosis assays measure different parameters, they are generally employed simultaneously for a comprehensive analysis of a sample population’s health. The balance between cell proliferation and apoptosis is vital for both development and normal tissue homeostasis. AAT Bioquest offers a complete set of reagents and assay kits that are compatible in a variety of samples for studying cell viability, cell cycle, and cell proliferation.