6-DTAF [6-(4,6-Dichlorotriazinyl)aminofluorescein] CAS 118357-32-7

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Physical properties

Molecular weight	495.28
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-DTAF [6-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 118357-32-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	201.906 µL	1.01 mL	2.019 mL	10.095 mL	20.191 mL
5 mM	40.381 µL	201.906 µL	403.812 µL	2.019 mL	4.038 mL
10 mM	20.191 µL	100.953 µL	201.906 µL	1.01 mL	2.019 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
6-FAM [6-Carboxyfluorescein] CAS 3301-79-9	493	517	83000	0.178
5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] CAS 51306-35-5	498	517	80000¹	0.35
6-CR110 [6-Carboxyrhodamine 110] Single isomer	499	525	84000	0.091
6-CR6G [6-Carboxyrhodamine 6G] Single isomer	522	546	94000	0.214
6-TAMRA [6-Carboxytetramethylrhodamine] CAS 91809-67-5	552	578	90000	0.178
6-ROX [6-Carboxy-X-rhodamine] CAS 194785-18-7	578	604	82000	0.168
6-TRITC [Tetramethylrhodamine-6-isothiocyanate] CAS 80724-20-5	544	570	100000	0.34

Images

Figure 1. Chemical structure for 6-DTAF [6-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 118357-32-7*

References

View all 30 references: Citation Explorer

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Application of a tetrazolium dye as an indicator of viability in anaerobic bacteria
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Authors: Rumbaut RE, Harris NR, Sial AJ, Huxley VH, Granger DN.
Journal: Am J Physiol (1999): H333

Collagen fiber sliding during ligament growth and contracture
Authors: Wood ML, Lester GE, Dahners LE.
Journal: J Orthop Res (1998): 438