6-DTAF [6-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 118357-32-7*

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Chemical structure for 6-DTAF [6-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 118357-32-7*
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1 g 205 $1950

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Ex/Em (nm)492/517
CAS #118357-32-7
Storage F/D/L
Category Hydroxy-Reactive Probes
Fluorescent Carbohydrate Labels
Related Fluoresceins
Hydroxy-Reactive Probes
Compared to 5-DTAF, the 6-isomer is more preferably used for labeling oligonucleotides and nucleic acids. Like 5-DTAF, 6-DTAF not only reacts with amino groups, but also reacts with thiol groups and even directly reacts with hydroxy groups such as polysaccharides and other alcohols in aqueous solution at pH above 9. Due to its high reactivity, 6-DTAF is also used to label carbohydrates.

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-DTAF [6-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 118357-32-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

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References & Citations

Fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture
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Journal: Anal Biochem (2006): 177

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Journal: J Ocul Pharmacol Ther (2004): 129

Fluorescent cellulose microfibrils as substrate for the detection of cellulase activity
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Journal: Biomacromolecules (2003): 481

Microbial activity in soils following steam treatment
Authors: Richardson RE, James CA, Bhupathiraju VK, Alvarez-Cohen L.
Journal: Biodegradation (2002): 285

Effect of collagenase inhibitors on corneal haze after PRK
Authors: Corbett MC, O'Brart DP, Patmore AL, Marshall J.
Journal: Exp Eye Res (2001): 253

Glutamic acid decarboxylase-like immunoreactive axon terminals in synaptic contact with mesencephalic trigeminal nucleus neurons in the rat
Authors: Chen P, Li J, Mizuno N.
Journal: Neurosci Lett (2001): 167

Redistribution of plasma-membrane surface molecules during formation of the Leishmania amazonensis-containing parasitophorous vacuole
Authors: Henriques C, de Souza W.
Journal: Parasitol Res (2000): 215

Application of a tetrazolium dye as an indicator of viability in anaerobic bacteria
Authors: Bhupathiraju VK, Hernandez M, Landfear D, Alvarez-Cohen L.
Journal: J Microbiol Methods (1999): 231

Leakage responses to L-NAME differ with the fluorescent dye used to label albumin
Authors: Rumbaut RE, Harris NR, Sial AJ, Huxley VH, Granger DN.
Journal: Am J Physiol (1999): H333

Collagen fiber sliding during ligament growth and contracture
Authors: Wood ML, Lester GE, Dahners LE.
Journal: J Orthop Res (1998): 438

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Additional Documents

Safety Data Sheet (SDS)

1. Oligonucleotide Labeling Reagents
2. Peptide Labeling Reagents

Certificate of Analysis