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AA-dUTP [Aminoallyl dUTP sodium salt] *4 mM in Tris Buffer (pH 7.5)* *CAS 936327-10-5*

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Physical properties
Molecular weight589.17
SolventWater
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134

OverviewpdfSDSpdfProtocol


CAS
936327-10-5
Molecular weight
589.17
The amine-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce amine-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. Aminoallyl dUTP can be readily incorporated into DNA through the conventional enzymatic incorporation techniques. The resulting amine-modified nucleic acids can then be labeled using any of amine-reactive fluorescent dyes, biotins and other amine-reactive reagents. The aminoallyl-modified nucleotides can be incorporated to extremely high and consistent levels compared to the tag-labeled uridine triphosphates that generally have higher stereo-hindrance. Subsequent reaction of the amine-modified nucleic acid with an excess of amine-reactive reagent achieves correspondingly high and consistent labeling efficiencies, regardless of the labeling reagent chosen. This two-step labeling method also eliminates the need to optimize an enzymatic reaction to accommodate different dye-modified nucleotides, which may incorporate at very different rates. This labeling method is widely used for both FISH probes and microarray-based experiments.

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Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of AA-dUTP [Aminoallyl dUTP sodium salt] *4 mM in Tris Buffer (pH 7.5)* *CAS 936327-10-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM169.73 µL848.651 µL1.697 mL8.487 mL16.973 mL
5 mM33.946 µL169.73 µL339.461 µL1.697 mL3.395 mL
10 mM16.973 µL84.865 µL169.73 µL848.651 µL1.697 mL

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Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
TF1-dUTP *1 mM in Tris Buffer (pH 7.5)*341448200000.187
TF2-dUTP *1 mM in Tris Buffer (pH 7.5)*503525750000.09
TF3-dUTP *1 mM in Tris Buffer (pH 7.5)*5545787500010.179
TF4-dUTP *1 mM in Tris Buffer (pH 7.5)*578602900000.436
TF5-dUTP *1 mM in Tris Buffer (pH 7.5)*6496642500000.027
Tetramethylrhodamine-dUTP *1 mM in Tris Buffer (pH 7.5)*552578900000.178
DEAC-dUTP *1 mM in Tris Buffer (pH 7.5)*411472-0.12

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References


View all 9 references: Citation Explorer
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114
A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685
Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518
Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405
Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229
A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145
Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166
Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2'-deoxyuridine-5'-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953