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Biotin-11-dATP

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Physical properties
Molecular weight882.71
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


Molecular weight
882.71
The biotin-modified deoxyadenosine 5'-triphosphates are widely used for a variety of non-radioactive DNA labeling reactions including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. The biotinylated probes have been shown to hybridize to homologous nucleic acid at the same rate and to the same extent as non-biotinylated probes. The hybridized biotinylated DNA probes can be detected by avidin and streptavidin. Biotin-11-dATP can be enzymatically incorporated into DNA via nick-translation, random priming, 3'-end terminal labeling or in the process of PCR. The number '11' is the number of carbon atoms in the backbone of the linker between dATP and biotin. The longer the linker is, the more effective interaction of biotin with avidin occurs. On the other hand, the shorter the linker is, the more effective incorporation of dATP into DNA. It is suggested the length of linker '11' is optimal for most applications. Biotin-11-dATP is used to produce biotinylated DNA probes in a variety of hybridization applications including Southern blots, Northern blots, dot blots, fixed cells, and tissues. It is chemically equivalent to NEL540001EA of PerkinElmer (PE).

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Biotin-11-dATP to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM113.287 µL566.437 µL1.133 mL5.664 mL11.329 mL
5 mM22.657 µL113.287 µL226.575 µL1.133 mL2.266 mL
10 mM11.329 µL56.644 µL113.287 µL566.437 µL1.133 mL

Molarity calculator

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References


View all 17 references: Citation Explorer
Measurement of differential chromatin interactions with absolute quantification of architecture (AQuA-HiChIP).
Authors: Gryder, Berkley E and Khan, Javed and Stanton, Benjamin Z
Journal: Nature protocols (2020): 1209-1236
Highly sensitive and selective electrochemical detection of Hg(2+) through surface-initiated enzymatic polymerization.
Authors: Mei, Chenyang and Lin, Dajie and Fan, Chengchao and Liu, Aili and Wang, Shun and Wang, Jichang
Journal: Biosensors & bioelectronics (2016): 105-110
Ultrasensitive electrochemical DNA sensor based on the target induced structural switching and surface-initiated enzymatic polymerization.
Authors: Wan, Ying and Wang, Pengjuan and Su, Yan and Zhu, Xinhua and Yang, Shulin and Lu, Jianxin and Gao, Jimin and Fan, Chunhai and Huang, Qing
Journal: Biosensors & bioelectronics (2014): 231-6
A surface-initiated enzymatic polymerization strategy for electrochemical DNA sensors.
Authors: Wan, Ying and Xu, Hui and Su, Yan and Zhu, Xinhua and Song, Shiping and Fan, Chunhai
Journal: Biosensors & bioelectronics (2013): 526-31
Oxidative DNA injury after experimental intracerebral hemorrhage.
Authors: Nakamura, Takehiro and Keep, Richard F and Hua, Ya and Hoff, Julian T and Xi, Guohua
Journal: Brain research (2005): 30-6
Impact of hypoglycemia and diabetes on CNS: correlation of mitochondrial oxidative stress with DNA damage.
Authors: Singh, Puneet and Jain, Anu and Kaur, Gurcharan
Journal: Molecular and cellular biochemistry (2004): 153-9
Oxidative brain injury from extravasated erythrocytes after intracerebral hemorrhage.
Authors: Wu, Jimin and Hua, Ya and Keep, Richard F and Schallert, Timothy and Hoff, Julian T and Xi, Guohua
Journal: Brain research (2002): 45-52
Detection of single- and double-strand DNA breaks after traumatic brain injury in rats: comparison of in situ labeling techniques using DNA polymerase I, the Klenow fragment of DNA polymerase I, and terminal deoxynucleotidyl transferase.
Authors: Clark RSB, and Chen, M and Kochanek, P M and Watkins, S C and Jin, K L and Draviam, R and Nathaniel, P D and Pinto, R and Marion, D W and Graham, S H
Journal: Journal of neurotrauma (2001): 675-89
Spatio-temporal profile of DNA fragmentation and its relationship to patterns of epileptiform activity following focally evoked limbic seizures.
Authors: Henshall, D C and Sinclair, J and Simon, R P
Journal: Brain research (2000): 290-302
Induction of oxidative DNA damage in the peri-infarct region after permanent focal cerebral ischemia.
Authors: Nagayama, T and Lan, J and Henshall, D C and Chen, D and O'Horo, C and Simon, R P and Chen, J
Journal: Journal of neurochemistry (2000): 1716-28