logo
AAT Bioquest

Calbryte™ 590, potassium salt

Fluorescence emission spectra of Calbryte&trade; 590 in solution containing 0 to 39 &micro;M free Ca<sup>2+</sup>.
Fluorescence emission spectra of Calbryte&trade; 590 in solution containing 0 to 39 &micro;M free Ca<sup>2+</sup>.
Fluorescence emission spectra of Calbryte&trade; 590 in solution containing 0 to 39 &micro;M free Ca<sup>2+</sup>.
Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Dissociation constant (Kd, nM)1400
Molecular weight1074.98
SolventWater
Spectral properties
Excitation (nm)581
Emission (nm)593
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Related products
Calbryte™ 520 AM
Calbryte™ 590 AM
Calbryte™ 630 AM
Calbryte™-520L AM
Calbryte™-520XL azide
Calbryte™-520XL AM
Calbryte™-520XL-Dextran
Chemical Phosphorylation Reagent I (CPR I)
Cell Meter™ Mitochondrial Hydroxyl Radical Detection Kit *Red Fluorescence*
Cal Green™ 1, hexapotassium salt
Cal Green™ 1, AM [Equivalent to Calcium Green-1, AM]
Cal-590™-Dextran Conjugate *MW 3,000*
Cal-590™-Dextran Conjugate *MW 10,000*
Cal-590™ AM
Cal-590™, sodium salt
Cal-630™ AM
Cal-630™, sodium salt
Cal-630™, potassium salt
Cal-630™-Dextran Conjugate *MW 3,000*
Cal-630™-Dextran Conjugate *MW 10,000*
Fluo-4 AM *Ultrapure Grade* *CAS 273221-67-3*
Fluo-4, Pentapotassium Salt
Cal Red™ R525/650 potassium salt
Cal Red™ R525/650 AM
Cal-520®-Dextran Conjugate *MW 3,000*
Cal-520®-Dextran Conjugate *MW 10,000*
Cal-520®-Biotin Conjugate
Cal-520®-Biocytin Conjugate
Cal-520® NHS Ester
Cal-520® maleimide
Fluo-3, AM *CAS 121714-22-5*
Fluo-3, AM *UltraPure grade* *CAS 121714-22-5*
Fluo-3, AM *Bulk package* *CAS 121714-22-5*
Fluo-3FF, AM *UltraPure grade* *Cell permeant*
Fluo-3, pentasodium salt
Fluo-3, pentapotassium salt
Fluo-3, pentaammonium salt
Fluo-3FF, pentapotassium salt
Fluo-8®, AM
Fluo-8®, sodium salt
Fluo-8®, potassium salt
Fluo-8H™, AM
Fluo-8H™, sodium salt
Fluo-8L™, AM
Fluo-8L™, sodium salt
Fluo-8L™, potassium salt
Fluo-8FF™, potassium salt
Fluo-8FF™, AM
Cal-520®, AM
Cal-520®, sodium salt
Cal-520®, potassium salt
Cal-520FF™, AM
Cal-520FF™, potassium salt
Screen Quest™ Fluo-8 Medium Removal Calcium Assay Kit *Optimized for Difficult Cell Lines*
Screen Quest™ Fluo-8 No Wash Calcium Assay Kit
Mag-Fluo-4 potassium salt
Mag-Fluo-4 AM
Fluo-2, potassium salt
Fluo-2, AM
Fluo-5F, AM *Cell permeant*
Fluo-5F, pentapotassium Salt *Cell impermeant*
Fluo-5N, AM *Cell permeant*
Fluo-5N, pentapotassium Salt *Cell impermeant*
Cal-520N™, AM
Cal-520N™, potassium salt
Screen Quest™ Fluo-4 No Wash Calcium Assay Kit
Screen Quest™ Calbryte-520 Probenecid-Free and Wash-Free Calcium Assay Kit
Screen Quest™ Calbryte-590 Probenecid-Free and Wash-Free Calcium Assay Kit
Cal-500™, potassium salt
Cal-500™ AM
Cal-670™, potassium salt
Cal-670™-Dextran Conjugate *MW 3,000*
Cal-670™-Dextran Conjugate *MW 10,000*
Cal-770™, potassium salt
Cal-770™-Dextran Conjugate *MW 3,000*
Cal-770™-Dextran Conjugate *MW 10,000*
Cal-520L®-Dextran Conjugate *MW 10,000*
RatioWorks™ Cal-520L®/Cy5-Dextran Conjugate *MW 10,000*
Cal-520L™ maleimide
RatioWorks™ Cal-520®/zFluor 647™ -Dextran Conjugate *MW 10,000*
Cal-590L® Dextran Conjugate *MW 10,000*
RatioWorks™ Cal-590L®/Cy5-Dextran Conjugate *MW 10,000*
Cal-520® amine
Cal-520® azide
Cal-520® alkyne
Show More (75)

OverviewpdfSDSpdfProtocol


Molecular weight
1074.98
Dissociation constant (Kd, nM)
1400
Excitation (nm)
581
Emission (nm)
593
The intracellular calcium flux assay is a widely used method in monitoring signal transduction pathways and high throughput screening of G protein"coupled receptors (GPCRs) and calcium channel targets. Followed by Rhod-2 being introduced in 1989, Rhod-4 and Cal-590 were later developed with improved signal/background ratio, and they became the widely used red fluorescent Ca2+ indicators for confocal microscopy, flow cytometry and high throughput screening applications. Calbryte™ 590 is a new generation of red fluorescent indicators for the measurement of intracellular calcium. Its greatly improved signal/background ratio and intracellular retention properties make Calbryte™ 590 the most robust red fluorescent indicator for evaluating GPCR and calcium channel targets as well as for screening their agonists and antagonists in live cells.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Calbryte™ 590, potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM93.025 µL465.125 µL930.25 µL4.651 mL9.302 mL
5 mM18.605 µL93.025 µL186.05 µL930.25 µL1.86 mL
10 mM9.302 µL46.512 µL93.025 µL465.125 µL930.25 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Excitation (nm)581
Emission (nm)593

Product Family


Images


Citations


View all 10 citations: Citation Explorer
Calreticulin regulates TGF-&beta;1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling
Authors: Wu, Yanjiao and Xu, Xiaoli and Ma, Lunkun and Yi, Qian and Sun, Weichao and Tang, Liling
Journal: The International Journal of Biochemistry &amp; Cell Biology (2017)
Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells
Authors: Lu, Jiang and Yao, Xue-qin and Luo, Xin and Wang, Yu and Chung, Sookja Kim and Tang, He-xin and Cheung, Chi Wai and Wang, Xian-yu and Meng, Chen and Li, Qing and others, undefined
Journal: Neural Regeneration Research (2017): 945
Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels
Authors: Yang, Gang and Xiao, Zhenghua and Ren, Xiaomei and Long, Haiyan and Ma, Kunlong and Qian, Hong and Guo, Yingqiang
Journal: Scientific Reports (2017): 41781
Dexmedetomidine reduces hypoxia/reoxygenation injury by regulating mitochondrial fission in rat hippocampal neurons
Authors: Liu, Jia and Du, Qing and Zhu, He and Li, Yu and Liu, Maodong and Yu, Shoushui and Wang, Shilei
Journal: Int J Clin Exp Med (2017): 6861--6868
Di (2-ethylhexyl) phthalate-induced apoptosis in rat INS-1 cells is dependent on activation of endoplasmic reticulum stress and suppression of antioxidant protection
Authors: Sun, Xia and Lin, Yi and Huang, Qiansheng and Shi, Junpeng and Qiu, Ling and Kang, Mei and Chen, Yajie and Fang, Chao and Ye, Ting and Dong, Sijun
Journal: Journal of cellular and molecular medicine (2015): 581--594
The effect of mitochondrial calcium uniporter on mitochondrial fission in hippocampus cells ischemia/reperfusion injury
Authors: Zhao, Lantao and Li, Shuhong and Wang, Shilei and Yu, Ning and Liu, Jia
Journal: Biochemical and biophysical research communications (2015): 537--542
Role of mitochondrial calcium uniporter in regulating mitochondrial fission in the cerebral cortexes of living rats
Authors: Liang, Nan and Wang, Peng and Wang, Shilei and Li, Shuhong and Li, Yu and Wang, Jinying and Wang, Min
Journal: Journal of Neural Transmission (2014): 593--600
Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells
Authors: Li, Qing and Lu, Jiang and Wang, Xianyu and others, undefined
Journal: Neural regeneration research (2014): 2002
Fungus induces the release of IL-8 in human corneal epithelial cells, via Dectin-1-mediated protein kinase C pathways.
Authors: Peng, Xu-Dong and Zhao, Gui-Qiu and Lin, Jing and Jiang, Nan and Xu, Qiang and Zhu, Cheng-Cheng and Qu, Jain-Qiu and Cong, Lin and Li, Hui
Journal: International journal of ophthalmology (2014): 441--447
Increased expression of cell adhesion molecule 1 by mast cells as a cause of enhanced nerve--mast cell interaction in a hapten-induced mouse model of atopic dermatitis
Authors: Hagiyama, M and Inoue, T and Furuno, T and Iino, T and Itami, S and Nakanishi, M and Asada, H and Hosokawa, Y and Ito, A
Journal: British Journal of Dermatology (2013): 771--778

References


View all 53 references: Citation Explorer
A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux
Authors: Bailey S, Macardle PJ.
Journal: J Immunol Methods (2006): 220
Functional fluo-3/AM assay on P-glycoprotein transport activity in L1210/VCR cells by confocal microscopy
Authors: Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.
Journal: Gen Physiol Biophys (2004): 357
Comparison of human recombinant adenosine A2B receptor function assessed by Fluo-3-AM fluorometry and microphysiometry
Authors: Patel H, Porter RH, Palmer AM, Croucher MJ.
Journal: Br J Pharmacol (2003): 671
Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics
Authors: Loughrey CM, MacEachern KE, Cooper J, Smith GL.
Journal: Cell Calcium (2003): 1
A sensitive method for the detection of foot and mouth disease virus by in situ hybridisation using biotin-labelled oligodeoxynucleotides and tyramide signal amplification
Authors: Zhang Z, Kitching P.
Journal: J Virol Methods (2000): 187
Kinetics of onset of mouse sperm acrosome reaction induced by solubilized zona pellucida: fluorimetric determination of loss of pH gradient between acrosomal lumen and medium monitored by dapoxyl (2-aminoethyl) sulfonamide and of intracellular Ca(2+) chang
Authors: Rockwell PL, Storey BT.
Journal: Mol Reprod Dev (2000): 335
MRP2, a human conjugate export pump, is present and transports fluo 3 into apical vacuoles of Hep G2 cells
Authors: Cantz T, Nies AT, Brom M, Hofmann AF, Keppler D.
Journal: Am J Physiol Gastrointest Liver Physiol (2000): G522
Use of co-loaded Fluo-3 and Fura Red fluorescent indicators for studying the cytosolic Ca(2+)concentrations distribution in living plant tissue
Authors: Walczysko P, Wagner E, Albrechtova JT.
Journal: Cell Calcium (2000): 23
[Ca2+]i following extrasystoles in guinea-pig trabeculae microinjected with fluo-3 - a comparison with frog skeletal muscle fibres
Authors: Wohlfart B., undefined
Journal: Acta Physiol Scand (2000): 1
Determination of the intracellular dissociation constant, K(D), of the fluo-3. Ca(2+) complex in mouse sperm for use in estimating intracellular Ca(2+) concentrations
Authors: Rockwell PL, Storey BT.
Journal: Mol Reprod Dev (1999): 418