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Cy7®-streptavidin conjugate

HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by Cy7®-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel.
HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by Cy7®-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel.
HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by Cy7®-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel.
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Physical properties
Molecular weight~52000
SolventWater
Spectral properties
Correction Factor (260 nm)0.05
Correction Factor (280 nm)0.036
Correction Factor (482 nm)0.0005
Correction Factor (565 nm)0.0193
Correction Factor (650 nm)0.165
Extinction coefficient (cm -1 M -1)250000
Excitation (nm)756
Emission (nm)779
Quantum yield0.3
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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OverviewpdfSDSpdfProtocol


Molecular weight
~52000
Correction Factor (260 nm)
0.05
Correction Factor (280 nm)
0.036
Correction Factor (482 nm)
0.0005
Correction Factor (565 nm)
0.0193
Correction Factor (650 nm)
0.165
Extinction coefficient (cm -1 M -1)
250000
Excitation (nm)
756
Emission (nm)
779
Quantum yield
0.3
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This Cy7-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with Cy7 covalently attached (as the fluorescent label). Our Cy7-streptavidin conjugate was prepared using AAT Bioquest's proprietary labeling technology. It demonstrated much brighter signal compared to the similar Cy7-streptavidin conjugates from other commercial sources, thus can significantly increase assay sensitivities. It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry with a Cy7 filter set. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.

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Spectral properties

Correction Factor (260 nm)0.05
Correction Factor (280 nm)0.036
Correction Factor (482 nm)0.0005
Correction Factor (565 nm)0.0193
Correction Factor (650 nm)0.165
Extinction coefficient (cm -1 M -1)250000
Excitation (nm)756
Emission (nm)779
Quantum yield0.3

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Cy7 biotin conjugate7567792500000.30.050.036
RPE-streptavidin conjugate56557419600000.82-0.175
APC-streptavidin conjugate651660730000--0.195
PerCP-streptavidin conjugate477678406000--0.22
FITC-streptavidin conjugate491516730000.92-0.35
Cy3®-streptavidin conjugate55556915000010.1510.070.073
Cy5®-streptavidin conjugate65167025000010.271, 0.420.020.03
XFD350-streptavidin conjugate *XFD350 Same Structure to Alexa Fluor™ 350*34344119000-0.250.19
XFD488-streptavidin conjugate *XFD488 Same Structure to Alexa Fluor™ 488*499520730000.9210.30.11
XFD594-streptavidin conjugate *XFD594 Same Structure to Alexa Fluor™ 594*590618920000.6610.430.56
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Citations


View all 3 citations: Citation Explorer
Persistent repression of tau in the brain using engineered zinc finger protein transcription factors
Authors: Wegmann, Susanne and DeVos, Sarah L and Zeitler, Bryan and Marlen, Kimberly and Bennett, Rachel E and Perez-Rando, Marta and MacKenzie, Danny and Yu, Qi and Commins, Caitlin and Bannon, Riley N and others,
Journal: Science advances (2021): eabe1611
Persistent repression of tau in the brain using engineered zinc finger protein transcription factors
Authors: Wegmann, Susanne and DeVos, Sarah L and Zeitler, Bryan and Marlen, Kimberly and Bennett, Rachel E and Perez-Rando, Marta and MacKenzie, Danny and Yu, Qi and Commins, Caitlin and Bannon, Riley N and others,
Journal: Science Advances (2021): eabe1611
Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996

References


View all 47 references: Citation Explorer
A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)
Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362
Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647
Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex
Authors: Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.
Journal: BMC Biochem (2010): 50
Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label
Authors: Sun M, Du L, Gao S, Bao Y, Wang S.
Journal: Steroids (2010): 400
Multimodality nuclear and fluorescence tumor imaging in mice using a streptavidin nanoparticle
Authors: Liang M, Liu X, Cheng D, Liu G, Dou S, Wang Y, Rusckowski M, Hnatowich DJ.
Journal: Bioconjug Chem (2010): 1385
Site-dependent excited-state dynamics of a fluorescent probe bound to avidin and streptavidin
Authors: Furstenberg A, Kel O, Gradinaru J, Ward TR, Emery D, Bollot G, Mareda J, Vauthey E.
Journal: Chemphyschem (2009): 1517
Influence of streptavidin on the absorption and fluorescence properties of cyanine dyes
Authors: Luschtinetz F, Dosche C, Kumke MU.
Journal: Bioconjug Chem (2009): 576
Fluorescent nanoscale detection of biotin-streptavidin interaction using near-field scanning optical microscopy
Authors: Park HK, Gokarna A, Hulme JP, Park HG, Chung BH.
Journal: Nanotechnology (2008): 235103
Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin, and biotin or biotin derivatives
Authors: Ebner A, Marek M, Kaiser K, Kada G, Hahn CD, Lackner B, Gruber HJ.
Journal: Methods Mol Biol (2008): 73