Cy7®-streptavidin conjugate

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<p>HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by Cy7®-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel.</p>
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Unit Size: Cat No: Price (USD): Qty:
1 mg 16914 $145


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Additional Ordering Information
Telephone: 1-800-990-8053
Fax: 1-408-733-1304
Email: sales@aatbio.com
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Overview

Ex/Em (nm)754/778
SolventWater
Storage Freeze (<-15 °C)
Minimize light exposure
Category Dye to Nucleic Acid Conjugation,
Biotin and Streptavidin,
Bioconjugation
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This Cy7-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with Cy7 covalently attached (as the fluorescent label). Our Cy7-streptavidin conjugate was prepared using AAT Bioquest's proprietary labeling technology. It demonstrated much brighter signal compared to the similar Cy7-streptavidin conjugates from other commercial sources, thus can significantly increase assay sensitivities. It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry with a Cy7 filter set. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.




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References

Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Hongyu Li, Hui Zhang, Shujun Zhao, Yun Shi, Junge Yao, Yanyan Zhang, Huanhuan Guo, Xingsuo Liu
Journal: Oncology letters (2015): 1989--1996






Resources
 
Safety Data Sheet (SDS)


Documents
1. Fluorescent Labeling Probes & Kits

Certificate of Analysis