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iFluor® 790 amine

Fluorescent dye amines are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester).
Fluorescent dye amines are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester).
Fluorescent dye amines are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester).
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Telephone1-800-990-8053
Fax1-800-609-2943
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Physical properties
Molecular weight1213.22
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.1
Correction Factor (280 nm)0.09
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)787
Emission (nm)812
Quantum yield0.131
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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iFluor® 405 goat anti-mouse IgG (H+L)
iFluor® 488 goat anti-mouse IgG (H+L)
iFluor® 514 goat anti-mouse IgG (H+L)
iFluor® 532 goat anti-mouse IgG (H+L)
iFluor® 555 goat anti-mouse IgG (H+L)
iFluor® 594 goat anti-mouse IgG (H+L)
iFluor® 633 goat anti-mouse IgG (H+L)
iFluor® 647 goat anti-mouse IgG (H+L)
iFluor® 680 goat anti-mouse IgG (H+L)
iFluor® 700 goat anti-mouse IgG (H+L)
iFluor® 750 goat anti-mouse IgG (H+L)
iFluor® 790 goat anti-mouse IgG (H+L)
iFluor® 350 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 405 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 488 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 514 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 532 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 555 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 594 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 633 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 647 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 680 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 700 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 750 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 790 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 350 goat anti-rabbit IgG (H+L)
iFluor® 405 goat anti-rabbit IgG (H+L)
iFluor® 488 goat anti-rabbit IgG (H+L)
iFluor® 514 goat anti-rabbit IgG (H+L)
iFluor® 532 goat anti-rabbit IgG (H+L)
iFluor® 555 goat anti-rabbit IgG (H+L)
iFluor® 594 goat anti-rabbit IgG (H+L)
iFluor® 633 goat anti-rabbit IgG (H+L)
iFluor® 647 goat anti-rabbit IgG (H+L)
iFluor® 680 goat anti-rabbit IgG (H+L)
iFluor® 700 goat anti-rabbit IgG (H+L)
iFluor® 750 goat anti-rabbit IgG (H+L)
iFluor® 790 goat anti-rabbit IgG (H+L)
iFluor® 350 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 405 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 488 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 514 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 532 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 555 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 594 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 633 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 647 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 680 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 700 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 750 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 790 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 350-streptavidin conjugate
iFluor® 405-streptavidin conjugate
iFluor® 488-streptavidin conjugate
iFluor® 514-streptavidin conjugate
iFluor® 532-streptavidin conjugate
iFluor® 555-streptavidin conjugate
iFluor® 594-streptavidin conjugate
iFluor® 633-streptavidin conjugate
iFluor® 647-streptavidin conjugate
iFluor® 680-streptavidin conjugate
iFluor® 700-streptavidin conjugate
iFluor® 750-streptavidin conjugate
iFluor® 750 RGD Conjugate
iFluor® 488 azide
iFluor® 405 hydrazide
iFluor® 800 acid
iFluor® 800 maleimide
iFluor® 810 acid
iFluor® 810 maleimide
iFluor® 820 acid
iFluor® 820 maleimide
iFluor® 860 acid
iFluor® 860 maleimide
iFluor® 546 goat anti-mouse IgG (H+L)
iFluor® 546 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 568 goat anti-mouse IgG (H+L)
iFluor® 568 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 546 goat anti-rabbit IgG (H+L)
iFluor® 546 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 568 goat anti-rabbit IgG (H+L)
iFluor® 568 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 555 alkyne
iFluor® 555 azide
iFluor® 546-streptavidin conjugate
iFluor® 568-streptavidin conjugate
iFluor® 532 maleimide
iFluor® 594 maleimide
iFluor® 488 alkyne
iFluor® 405 maleimide
iFluor® 430 maleimide
iFluor® 568 maleimide
iFluor® 633 maleimide
iFluor® 450 maleimide
iFluor® 350 Styramide *Superior Replacement for Alexa Fluor 350 tyramide*
iFluor® 488 Styramide *Superior Replacement for Alexa Fluor 488 tyramide and Opal 520*
iFluor® 546 Styramide *Superior Replacement for Alexa Fluor 546 tyramide*
iFluor® 555 Styramide *Superior Replacement for Alexa Fluor 555 tyramide and Opal 570*
iFluor® 568 Styramide *Superior Replacement for Alexa Fluor 568 tyramide*
iFluor® 594 Styramide *Superior Replacement for Alexa Fluor 594 tyramide*
iFluor® 647 Styramide *Superior Replacement for Alexa Fluor 647 tyramide*
iFluor® 680 Styramide *Superior Replacement for Alexa Fluor 680 tyramide and Opal 690*
iFluor® 700 Styramide *Superior Replacement for Alexa Fluor 700 tyramide*
iFluor® 750 Styramide *Superior Replacement for Alexa Fluor 750 tyramide*
iFluor® 555 Tyramide
iFluor® 647 Tyramide
iFluor® 350 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor® 488 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor® 555 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor® 594 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor® 647 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor® 350 PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor® 488 PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor® 555 PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor® 594 PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor® 647 PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor® 840 acid
iFluor® 790-streptavidin conjugate
iFluor® 800-streptavidin conjugate
iFluor® 820-streptavidin conjugate
iFluor® 840-streptavidin conjugate
iFluor® 860-streptavidin conjugate
iFluor® 488-Wheat Germ Agglutinin (WGA) Conjugate
iFluor® 555-Wheat Germ Agglutinin (WGA) Conjugate
iFluor® 594-Wheat Germ Agglutinin (WGA) Conjugate
iFluor® 647-Wheat Germ Agglutinin (WGA) Conjugate
iFluor® 488-Concanavalin A Conjugate
iFluor® 555-Concanavalin A Conjugate
iFluor® 594-Concanavalin A Conjugate
iFluor® 647-Concanavalin A Conjugate
iFluor® 350 Tyramide
iFluor® 546 Tyramide
iFluor® 568 Tyramide
iFluor® 594 Tyramide
iFluor® 460 maleimide
iFluor® 488 TCO
iFluor® 555 TCO
iFluor® 594 TCO
iFluor® 647 TCO
iFluor® 488 Tetrazine
iFluor® 555 Tetrazine
iFluor® 594 Tetrazine
iFluor® 647 Tetrazine
iFluor® 440-dUTP *1 mM in Tris Buffer (pH 7.5)*
iFluor® 665 maleimide
iFluor®488-dUTP *1 mM in Tris Buffer (pH 7.5)*
iFluor® 450 Styramide *Superior Replacement for Opal Polaris 480*
iFluor® 633 tyramide
iFluor® 514 Styramide *Superior Replacement for Opal 540*
iFluor® 532 Styramide
iFluor® 633 Styramide *Superior Replacement for Opal 650*
iFluor® 440 Styramide
iFluor® 460 Styramide
iFluor® 610 Styramide
iFluor® 660 Styramide
iFluor® 405 Styramide
iFluor®488-PEG12-dUTP *1 mM in Tris Buffer (pH 7.5)*
iFluor®555-PEG12-dUTP *1 mM in Tris Buffer (pH 7.5)*
iFluor®647-PEG12-dUTP *1 mM in Tris Buffer (pH 7.5)*
iFluor® 800 goat anti-mouse IgG (H+L)
iFluor® 800 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 810 goat anti-mouse IgG (H+L)
iFluor® 810 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 820 goat anti-mouse IgG (H+L)
iFluor® 820 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 840 goat anti-mouse IgG (H+L)
iFluor® 840 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 860 goat anti-mouse IgG (H+L)
iFluor® 860 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 800 goat anti-rabbit IgG (H+L)
iFluor® 800 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 810 goat anti-rabbit IgG (H+L)
iFluor® 810 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 820 goat anti-rabbit IgG (H+L)
iFluor® 820 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 840 goat anti-rabbit IgG (H+L)
iFluor® 840 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 860 goat anti-rabbit IgG (H+L)
iFluor® 860 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 430 Tyramide *Superior Replacement for Opal 480*
iFluor® 450 Tyramide *Superior Replacement for Opal 480*
iFluor® 546 maleimide
iFluor® 840 maleimide
iFluor® 770 maleimide
iFluor® 780 maleimide
iFluor® 350 succinimidyl ester
iFluor® 405 succinimidyl ester
iFluor® 488 succinimidyl ester
iFluor® 514 succinimidyl ester
iFluor® 532 succinimidyl ester
iFluor® 555 succinimidyl ester
iFluor® 594 succinimidyl ester
iFluor® 633 succinimidyl ester
iFluor® 647 succinimidyl ester
iFluor® 660 succinimidyl ester
iFluor® 680 succinimidyl ester
iFluor® 700 succinimidyl ester
iFluor® 750 succinimidyl ester
iFluor® 610 succinimidyl ester
iFluor® 710 succinimidyl ester
iFluor® 800 succinimidyl ester
iFluor® 810 succinimidyl ester
iFluor® 820 succinimidyl ester
iFluor® 860 succinimidyl ester
iFluor® 546 succinimidyl ester
iFluor® 568 succinimidyl ester
iFluor® 430 succinimidyl ester
iFluor® 450 succinimidyl ester
iFluor® 840 succinimidyl ester
iFluor® 560 succinimidyl ester
iFluor® 670 succinimidyl ester
iFluor® 460 succinimidyl ester
iFluor® 440 succinimidyl ester
iFluor® 665 succinimidyl ester
iFluor® 690 succinimidyl ester
iFluor® Ultra 594 succinimidyl ester
iFluor® Ultra 647 succinimidyl ester
iFluor® Ultra 750 succinimidyl ester
iFluor® 720 succinimidyl ester
iFluor® 740 succinimidyl ester
iFluor® 597 succinimidyl ester
iFluor® 770 succinimidyl ester
iFluor® 780 succinimidyl ester
iFluor® 570 succinimidyl ester
iFluor® 830 acid
iFluor® 830 maleimide
iFluor® 830 succinimidyl ester
iFluor™ 405 azide
iFluor® 514 maleimide
iFluor® 660 maleimide
iFluor® 670 maleimide
iFluor® 675 succinimidyl ester
iFluor® 620 succinimidyl ester
iFluor® 560-dUTP *1 mM in Tris Buffer (pH 7.5)*
iFluor® 750-Concanavalin A Conjugate
iFluor® 605 succinimidyl ester
iFluor® 605 maleimide
iFluor® 625 succinimidyl ester
iFluor® 625 maleimide
iFluor® 510 succinimidyl ester
iFluor® 510 maleimide
iFluor® 540 succinimidyl ester
iFluor® 540 maleimide
iFluor® 350-Wheat Germ Agglutinin (WGA) Conjugate
iFluor® 532-Wheat Germ Agglutinin (WGA) Conjugate
iFluor® 680-Wheat Germ Agglutinin (WGA) Conjugate
iFluor® 700-Wheat Germ Agglutinin (WGA) Conjugate
iFluor® 750-Wheat Germ Agglutinin (WGA) Conjugate
iFluor® 790-Wheat Germ Agglutinin (WGA) Conjugate
iFluor® 570 Styramide *Superior Replacement for Alexa Fluor 568 tyramide*
iFluor® 670 Styramide *Replacement for Opal 690*
iFluor® 445 succinimidyl ester
iFluor® 500 succinimidyl ester
iFluor® 680 Tyramide *Superior Replacement for Opal 690*
iFluor® 720 maleimide
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OverviewpdfSDSpdfProtocol


Molecular weight
1213.22
Correction Factor (260 nm)
0.1
Correction Factor (280 nm)
0.09
Extinction coefficient (cm -1 M -1)
2500001
Excitation (nm)
787
Emission (nm)
812
Quantum yield
0.131
In vivo fluorescence imaging uses a sensitive camera to detect the fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emission at the near-infrared (NIR) region are generally preferred, including widely used small indocarbocyanine dyes. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further emerging developments aim to achieve high-resolution, multimodality, and lifetime-based in vivo fluorescence imaging. Our iFluor® 790 is designed to label proteins and other biomolecules with near-infrared fluorescence. Conjugates prepared with iFluor® 790 have excitation and emission spectra similar to that of indocyanine green (ICG) and the IRDye® 800, with 787/812 nm excitation/emission maxima. iFluor® 790 dye emission is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in-vivo imaging applications or other imaging applications requiring NIR detections, such as the two-color western applications with the LI-COR® Odyssey® infrared imaging system.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 790 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM82.425 µL412.126 µL824.253 µL4.121 mL8.243 mL
5 mM16.485 µL82.425 µL164.851 µL824.253 µL1.649 mL
10 mM8.243 µL41.213 µL82.425 µL412.126 µL824.253 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.1
Correction Factor (280 nm)0.09
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)787
Emission (nm)812
Quantum yield0.131

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 350 amine3454502000010.9510.830.23
iFluor® 405 amine4034273700010.9110.480.77
iFluor® 488 amine4915167500010.910.210.11
iFluor® 555 amine55757010000010.6410.230.14
iFluor® 647 amine65667025000010.2510.030.03
iFluor® 660 amine66367825000010.2610.070.08
iFluor® 680 amine68470122000010.2310.0970.094
iFluor® 700 amine69071322000010.2310.090.04
iFluor® 710 amine71773919000010.6010.120.07
iFluor® 750 amine75777927500010.1210.0440.039
iFluor® 790 Styramide *Superior Replacement for Alexa Fluor 790 tyramide*78781225000010.1310.10.09
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Images


Citations


View all 1 citations: Citation Explorer
Nanovesicle delivery to the liver via retinol binding protein and platelet-derived growth factor receptors: how targeting ligands affect biodistribution
Authors: Hsu, Ching-Yun and Chen, Chun-Han and Aljuffali, Ibrahim A and Dai, You-Shan and Fang, Jia-You
Journal: Nanomedicine (2017)

References


View all 18 references: Citation Explorer
A target cell-specific activatable fluorescence probe for in vivo molecular imaging of cancer based on a self-quenched avidin-rhodamine conjugate
Authors: Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Shin IS, Paik CH, Choyke PL, Kobayashi H.
Journal: Cancer Res (2007): 2791
Fluorescence imaging in vivo: recent advances
Authors: Rao J, Dragulescu-Andrasi A, Yao H.
Journal: Curr Opin Biotechnol (2007): 17
Ex vivo fluorescence imaging of normal and malignant urothelial cells to enhance early diagnosis
Authors: Steenkeste K, Lecart S, Deniset A, Pernot P, Eschwege P, Ferlicot S, Leveque-Fort S, Bri and et R, Fontaine-Aupart MP.
Journal: Photochem Photobiol (2007): 1157
In vivo monitoring the fate of Cy5.5-Tat labeled T lymphocytes by quantitative near-infrared fluorescence imaging during acute brain inflammation in a rat model of experimental autoimmune encephalomyelitis
Authors: Berger C, Gremlich HU, Schmidt P, Cannet C, Kneuer R, Hiest and P, Rausch M, Rudin M.
Journal: J Immunol Methods (2007): 65
A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice
Authors: Bonano VI, Oltean S, Garcia-Blanco MA.
Journal: Nat Protoc (2007): 2166
In vivo imaging of the bronchial wall microstructure using fibered confocal fluorescence microscopy
Authors: Thiberville L, Moreno-Swirc S, Vercauteren T, Peltier E, Cave C, Bourg Heckly G.
Journal: Am J Respir Crit Care Med (2007): 22
In Vivo Fluorescence Microscopic Imaging for Dynamic Quantitative Assessment of Intestinal Mucosa Permeability in Mice
Authors: Szabo A, Vollmar B, Boros M, Menger MD.
Journal: J Surg Res. (2007)
In vivo spectral fluorescence imaging of submillimeter peritoneal cancer implants using a lectin-targeted optical agent
Authors: Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Krishna MC, Choyke PL, Kobayashi H.
Journal: Neoplasia (2006): 607
In vivo imaging of green fluorescent protein-expressing cells in transgenic animals using fibred confocal fluorescence microscopy
Authors: Al-Gubory KH, Houdebine LM.
Journal: Eur J Cell Biol (2006): 837
In vivo near-infrared fluorescence imaging of integrin alphavbeta3 in an orthotopic glioblastoma model
Authors: Hsu AR, Hou LC, Veeravagu A, Greve JM, Vogel H, Tse V, Chen X.
Journal: Mol Imaging Biol (2006): 315