iFluor®488-PEG12-dUTP 1 mM in Tris Buffer (pH 7.5)

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	2274.60
Solvent	Water

Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

2274.60

Correction Factor (260 nm)

0.21

Correction Factor (280 nm)

0.11

Extinction coefficient (cm ^-1 M ^-1)

75000¹

Excitation (nm)

491

Emission (nm)

516

Quantum yield

0.9¹

The dye-modified deoxyuridine 5'-triphosphates are one of the most common methods to produce dye-labelled DNA via the conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This iFluor®488-PEG12-dUTP conjugate has the same spectral properties to ChromaTide™Alexa Fluor 488-dUTP. Its PEG12 spacer makes it much more effectively incorporated into DNAs by polymerase. It is used as a green fluorescence color with SpectrumGreen™ filter set. It provides a bright signal that has high photostability and is not affected by pH. ChromaTide™ and Alexa Fluor™ are the trademarks of ThermoFisher, and SpectrumGreen™ is the trademark of Vysis.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of iFluor®488-PEG12-dUTP *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	43.964 µL	219.819 µL	439.638 µL	2.198 mL	4.396 mL
5 mM	8.793 µL	43.964 µL	87.928 µL	439.638 µL	879.275 µL
10 mM	4.396 µL	21.982 µL	43.964 µL	219.819 µL	439.638 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)	Correction Factor (656 nm)
iFluor®555-PEG12-dUTP 1 mM in Tris Buffer (pH 7.5)	557	570	100000¹	0.64¹	0.23	0.14	-
iFluor®647-PEG12-dUTP 1 mM in Tris Buffer (pH 7.5)	656	670	250000¹	0.25¹	0.03	0.03	0.0793

Images

Figure 1. Chemical structure for iFluor®488-PEG12-dUTP *1 mM in Tris Buffer (pH 7.5)*.

References

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Journal: Experimental and therapeutic medicine (2019): 3429-3440

Activation of β-catenin signaling in aggrecan-expressing cells in temporomandibular joint causes osteoarthritis-like defects.
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Adenovirus-delivered wwox inhibited lung cancer growth in vivo in a mouse model.
Authors: Zhou, Y and Shou, F and Zhang, H and You, Q
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Corn silk maysin induces apoptotic cell death in PC-3 prostate cancer cells via mitochondria-dependent pathway.
Authors: Lee, Jisun and Lee, Seul and Kim, Sun-Lim and Choi, Ji Won and Seo, Jeong Yeon and Choi, Doo Jin and Park, Yong Il
Journal: Life sciences (2014): 47-55

Hepatoprotective properties of sesamin against CCl4 induced oxidative stress-mediated apoptosis in mice via JNK pathway.
Authors: Ma, Jie-Qiong and Ding, Jie and Zhang, Li and Liu, Chan-Min
Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (2014): 41-8

Inhibition of APE1/Ref-1 redox activity rescues human retinal pigment epithelial cells from oxidative stress and reduces choroidal neovascularization.
Authors: Li, Y and Liu, X and Zhou, T and Kelley, M R and Edwards, P and Gao, H and Qiao, X
Journal: Redox biology (2014): 485-94

Immunoliposome co-delivery of bufalin and anti-CD40 antibody adjuvant induces synergetic therapeutic efficacy against melanoma.
Authors: Li, Ying and Yuan, Jiani and Yang, Qian and Cao, Wei and Zhou, Xuanxuan and Xie, Yanhua and Tu, Honghai and Zhang, Ya and Wang, Siwang
Journal: International journal of nanomedicine (2014): 5683-700

Plasma rich in growth factors (PRGF-Endoret) stimulates corneal wound healing and reduces haze formation after PRK surgery.
Authors: Anitua, E and Muruzabal, F and Alcalde, I and Merayo-Lloves, J and Orive, G
Journal: Experimental eye research (2013): 153-61

Inhalation of carbon monoxide following resuscitation ameliorates hemorrhagic shock-induced lung injury.
Authors: Kawanishi, Susumu and Takahashi, Toru and Morimatsu, Hiroshi and Shimizu, Hiroko and Omori, Emiko and Sato, Kenji and Matsumi, Masaki and Maeda, Shigeru and Nakao, Atsunori and Morita, Kiyoshi
Journal: Molecular medicine reports (2013): 3-10