Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

Signal Guard™ HRP reaction stopping solution

Application of HRP Reaction Stop Solution on HRP coupled glucose detection reaction. Two parallel reactions containing 15 µM Glucose were initiated by adding 50 µL assay mixture containing: 0.5mU/mL HRP, Amplex® Red, and 0.5mU/ml Glucose Oxidase. Reactions were incubated at room temperature for 5 mins and then 20 µL 1X Stop Reagent was added to one reaction, and 20 µL dd H2O to the other reaction. The plots demonstrated that the reaction is completely inhibited by Signal Guard™ HRP Reaction Stopping Solution.
Application of HRP Reaction Stop Solution on HRP coupled glucose detection reaction. Two parallel reactions containing 15 µM Glucose were initiated by adding 50 µL assay mixture containing: 0.5mU/mL HRP, Amplex® Red, and 0.5mU/ml Glucose Oxidase. Reactions were incubated at room temperature for 5 mins and then 20 µL 1X Stop Reagent was added to one reaction, and 20 µL dd H2O to the other reaction. The plots demonstrated that the reaction is completely inhibited by Signal Guard™ HRP Reaction Stopping Solution.
Ordering information
Price ()
Catalog Number11020
Unit Size
Find Distributor
Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


HRP coupling reactions provide sensitive biomolecular assays based on hydrogen peroxide- generating enzyme systems linked to peroxidase- mediated oxidation. Fluorogenic HRP substrates are preferred to use for enhancing assay sensitivities. Among them, the most commonly used HRP substrates include ADHP (also called Amplex® Red, #11000), Amplex® UltraRed and Amplite® Red. Typically, detection reactions are performed in microplate wells and are initiated by adding a fluorogenic HRP substrate, resulting in a continuous fluorescence increase. It is critical to ensure that the timing of the standard and unknown sample measurements is the same. Our Signal Guard™ HRP reaction stopping solution provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable. The Signal Guard™ HRP reaction stopping solution is designed for use in conjunction with ADHP (Amplex® Red ), Amplite® and Amplex®UltraRed fluorogenic substrates. Under the same conditions, Signal Guard™ HRP reaction stopping solution significantly outperforms the Amplex® Red/UltraRed Stop Reagent (#A33855) from ThermoFisher. Our Signal Guard™ HRP reaction stopping solution can also be used in other HRP reaction systems.

Citations


View all 10 citations: Citation Explorer
Patterned Photonic Nitrocellulose for Pseudo-Paper ELISA
Authors: Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze
Journal: Analytical Chemistry (2017)
Spinal Cord Inflammation: Molecular Imaging after Thoracic Aortic Ischemia Reperfusion Injury
Authors: Albadawi, Hassan and Chen, John W and Oklu, Rahmi and Wu, Yue and Wojtkiewicz, Gregory and Pulli, Benjamin and Milner, John D and Cambria, Richard P and Watkins, Michael T
Journal: Radiology (2016): 152222
Myeloperoxidase--Hepatocyte--Stellate Cell Cross Talk Promotes Hepatocyte Injury and Fibrosis in Experimental Nonalcoholic Steatohepatitis
Authors: Pulli, Benjamin and Ali, Muhammad and Iwamoto, Yoshiko and Zeller, Matthias WG and Schob, Stefan and Linnoila, Jenny J and Chen, John W
Journal: Antioxidants & redox signaling (2015): 1255--1269
Myeloperoxidase Nuclear Imaging for Epileptogenesis
Authors: Zhang, Yinian and Seeburg, Daniel P and Pulli, Benjamin and Wojtkiewicz, Gregory R and Bure, Lionel and Atkinson, Wendy and Schob, Stefan and Iwamoto, Yoshiko and Ali, Muhammad and Zhang, Wei and others, undefined
Journal: Radiology (2015): 822--830
Ordered cleavage of myeloperoxidase ester bonds releases active site heme leading to inactivation of myeloperoxidase by benzoic acid hydrazide analogs
Authors: Huang, Jiansheng and Smith, Forrest and Panizzi, Peter
Journal: Archives of biochemistry and biophysics (2014): 74--85
Raising the shields: PCR in the presence of metallic surfaces protected by tailor-made coatings
Authors: Scherag, Frank D and Br, undefined and stetter, Thomas and Rühe, Jürgen
Journal: Colloids and Surfaces B: Biointerfaces (2014): 576--582
Measuring myeloperoxidase activity in biological samples
Authors: Pulli, Benjamin and Ali, Muhammad and Forghani, Reza and Schob, Stefan and Hsieh, Kevin LC and Wojtkiewicz, Gregory and Linnoila, Jenny J and Chen, John W
Journal: PLoS One (2013): e67976
Micro-volume wall-less immunoassays using patterned planar plates
Authors: Kozak, Katherine R and Wang, Jianyong and Lye, Melvin and Takkar, Rashi and Kim, Namyong and Lee, Hyunjae and Jeon, Noo Li and Lin, Kedan and Zhang, Crystal and Wong, Wai Lee T and others, undefined
Journal: Lab on a Chip (2013): 1342--1350
Distinguishing inflammation from tumor and peritumoral edema by myeloperoxidase magnetic resonance imaging
Authors: Kleijn, Anne and Chen, John W and Buhrman, Jason S and Wojtkiewicz, Gregory R and Iwamoto, Yoshiko and Lamfers, Martine L and Stemmer-Rachamimov, Anat O and Rabkin, Samuel D and Weissleder, Ralph and Martuza, Robert L and others, undefined
Journal: Clinical Cancer Research (2011): 4484--4493

References


View all 61 references: Citation Explorer
Volatile fatty acid-sensing system involving coenzyme-A transferase
Authors: Rajashekhara E, Hosoda A, Sode K, Ikenaga H, Watanabe K.
Journal: Biotechnol Prog (2006): 334
Galactose oxidase action on galactose containing glycolipids--a fluorescence method
Authors: Fortelius M, Mattjus P.
Journal: Chem Phys Lipids (2006): 103
Effects of chronic ethanol administration on the activities and relative synthetic rates of myelin and synaptosomal plasma membrane-associated sialidase in the rat brain
Authors: Azuine MA, Patel SJ, Lakshman MR.
Journal: Neurochem Int (2006): 67
Mitochondrial reactive oxygen species in mice lacking superoxide dismutase 2: attenuation via antioxidant treatment
Authors: Morten KJ, Ackrell BA, Melov S.
Journal: J Biol Chem (2006): 3354
A new, sensitive method for enzyme kinetic studies of scarce glucosides
Authors: Mazura P, Fohlerova R, Brzobohaty B, Kiran NS, J and a L., undefined
Journal: J Biochem Biophys Methods (2006): 55
Evaluation of assays for the measurement of bovine neutrophil reactive oxygen species
Authors: Rinaldi M, Moroni P, Paape MJ, Bannerman DD.
Journal: Vet Immunol Immunopathol. (2006)
Kinetic analysis of semisynthetic peroxidase enzymes containing a covalent DNA-heme adduct as the cofactor
Authors: Fruk L, Muller J, Niemeyer CM.
Journal: Chemistry (2006): 7448
K+-independent actions of diazoxide question the role of inner membrane KATP channels in mitochondrial cytoprotective signaling
Authors: Drose S, Br and t U, Hanley PJ.
Journal: J Biol Chem (2006): 23733
A Role for Mitochondrial Dysfunction in Perpetuating Radiation-Induced Genomic Instability
Authors: Kim GJ, Fiskum GM, Morgan WF.
Journal: Cancer Res (2006): 10377
Sexual Dimorphism in Oxidant Status in Spontaneously Hypertensive Rats
Authors: Sullivan JC, Sasser JM, Pollock JS.
Journal: Am J Physiol Regul Integr Comp Physiol. (2006)