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AAT Bioquest

XFD680 Anti-human CD3 Antibody *UCHT1, XFD680 Same Structure to Alexa Fluor™ 680*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Correction Factor (260 nm)0.00
Correction Factor (280 nm)0.05
Extinction coefficient (cm -1 M -1)184000
Excitation (nm)681
Emission (nm)704
Quantum yield0.361
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
T3E
Clone
UCHT1
Host
mouse
Reactivity
human
Application
Flow Cytometry (FACS); Fluorescence Imaging
Correction Factor (260 nm)
0.00
Correction Factor (280 nm)
0.05
Extinction coefficient (cm -1 M -1)
184000
Excitation (nm)
681
Emission (nm)
704
Quantum yield
0.361
The UCHT1 monoclonal antibody binds to human CD3e, a 20 kD member of the Ig superfamily commonly found on the surface of tregs, thymocytes, nkt cells and thymocytes (differentiation dependent)s. In many organisms, CD3 enhances interleukin-4 production, is an inhibitor of gene expression and negatively regulates smoothened signaling pathway. Also, it plays a role in essential cellular pathways, for instance, the cell surface receptor signaling pathway, T cell receptor signaling pathway and apoptotic signaling pathway. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands like TCR. CD3 is a very popular antibody target, with over 80000 publications in the last decade. CD3e is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to XFD680 (ex/em = 681/704 nm). XFD680 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 680 (Alexa Fluor® is the trademark of ThermoFisher). It is compatible with the 642 nm laser and 702/87 nm bandpass filter (for example, as in the Luminex Amnis CellStream).

Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.00
Correction Factor (280 nm)0.05
Extinction coefficient (cm -1 M -1)184000
Excitation (nm)681
Emission (nm)704
Quantum yield0.361

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd3e antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.