Application Notes

General Properties Appearance: white crystal Solvents: dimethylsulfoxide (DMSO) or dimethylformamide (DMF) Note: SMCC Plus™ is soluble in water and many other aqueous buffers, in which it is less stable than in DMSO or DMF. Thus it is not recommended to make buffer stock solutions for repeated use.…

Categories: Antibody & Protein Labeling
Posted January 11, 2022

Introduction Maleimide groups rapidly and selectively react with thiols, forming stable thioether bonds. This characteristic has led to their widespread use in the conjugation of proteins to proteins or proteins to other biomolecules. Sensitive assays of maleimide are required to accurately…

Categories: Antibody & Protein Labeling
Posted July 15, 2020

Introduction iFluor® dyes are a series of excellent fluorescent labeling dyes that span the full visible spectrum. All the iFluor® dyes have excellent water solubility. Their hydrophilic property minimizes the use of organic solvents. The iFluor® dyes also have much better labeling performance…

Categories: Antibody & Protein Labeling
Posted January 10, 2020

Introduction SMCC heterobifunctional crosslinkers are often used in the preparation of PE, APC and tandem dye antibody conjugates. Although and established technique, SMCC-based conjugation suffers from several drawbacks, including: (1) poor conjugation efficiency (~30% yield), (2) requires…

Categories: Antibody & Protein Labeling
Posted December 3, 2019

Introduction SMCC heterobifunctional crosslinkers are often used in the preparation of PE, APC and tandem dye antibody conjugates. Although and established technique, SMCC-based conjugation suffers from several drawbacks, including: (1) poor conjugation efficiency (~30% yield), (2) requires…

Categories: Antibody & Protein Labeling
Posted November 26, 2019

AAT Bioquest iFluor® dyes are a series of highly water-soluble fluorescent dyes for labeling biomolecules, such as proteins and antibodies. Available in a variety of excitation-emission profiles, spanning the UV-visible to near-infrared spectrum, there is an iFluor® dye to match any instrument…

Categories: Antibody & Protein Labeling
Posted August 28, 2019

Introduction iFluor® dyes are a series of excellent fluorescent labeling dyes that span the full visible spectrum. All the iFluor® dyes have excellent water solubility. Their hydrophilic property minimizes the use of organic solvents. The iFluor® dyes also have much better labeling performance…

Categories: Antibody & Protein Labeling
Posted June 25, 2019

The phycobiliproteins are composed of a number of subunits, each having a protein backbone to which linear tetrapyrrole chromophores are covalently bound. Phycoerythrins (red) and phycocyanins (blue) are the two major classes of phycobiliproteins. The Absorption maxima for phycoerythrins (PE)…

Categories: Antibody & Protein Labeling
Posted July 23, 2012

Rapid Protein Crosslinking Method for Labeling & Modifying Antibodies Crosslinking is a technique that chemically joins two or more proteins together, such as an antibody and an enzyme, by a covalent bond. Crosslinking reagents consist of reactive moieties that modify and attach to specific functional…

Categories: Antibody & Protein Labeling
Posted September 12, 2017

Introduction The covalent conjugation of a large biomolecule to another macromolecule, such as an antibody to an enzyme, or an enzyme to DNA is an important task for researchers. There are a few methods available for crosslinking two biomolecules. One common method is to use a small crosslinker (such…

Categories: Antibody & Protein Labeling
Posted May 26, 2015