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AssayWise Letters 2019, Vol. 8(2)
In this issue
A Simple End-Point Calcium Assay Using a Green Fluorescent Indicator Fluo-8E™, AM
NAD+ Integration in Cellular Pathways
Power Styramide™ Signal Amplification a Superior Alternative to Tyramide Signal Amplification
Fluorescent Detection of Cell Senescence For Flow Cytometry and Imaging Applications
AssayWise Letters 2019, Vol. 8(2)
Featured Article
A Simple End-Point Calcium Assay Using a Green Fluorescent Indicator Fluo-8E™, AM
The intracellular release of calcium from internal stores upon GPCR stimulation is transient. To study this transient calcium signaling in a microplate format, a fluorescence plate reader that has a built-in liquid handling system and kinetic reading capability is often required. This limits the usage of the conventional fluorescence plate readers to conduct studies on calcium signaling. We report the development of an endpoint calcium assay that can be used to monitor the effects of GPCR modulators in calcium response cell-based assays with conventional fluorescence microplate readers…
The intracellular release of calcium from internal stores upon GPCR stimulation is transient. To study this transient calcium signaling in a microplate format, a fluorescence plate reader that has a built-in liquid handling system and kinetic reading capability is often required. This limits the usage of the conventional fluorescence plate readers to conduct studies on calcium signaling. We report the development of an endpoint calcium assay that can be used to monitor the effects of GPCR modulators in calcium response cell-based assays with conventional fluorescence microplate readers…
NAD+ Integration in Cellular Pathways
Cellular metabolism is the beginning of all other activities in living cells, providing energy for the millions of biochemical reactions that take place in any given moment. As such, any dysfunction in this process has immediate effects both on an intracellular scale and for overall human health.…
Cellular metabolism is the beginning of all other activities in living cells, providing energy for the millions of biochemical reactions that take place in any given moment. As such, any dysfunction in this process has immediate effects both on an intracellular scale and for overall human health.…
Power Styramide™ Signal Amplification a Superior Alternative to Tyramide Signal Amplification
Similar to tyramide signal amplification, Power Styramide™ Signal Amplification (PSA™) utilizes the catalytic activity of horseradish peroxidase (HRP) for the covalent deposition of iFluor™ dye labeled-Styramide™ radicals onto a target protein or nucleic acid sequence in situ. By combining…
Similar to tyramide signal amplification, Power Styramide™ Signal Amplification (PSA™) utilizes the catalytic activity of horseradish peroxidase (HRP) for the covalent deposition of iFluor™ dye labeled-Styramide™ radicals onto a target protein or nucleic acid sequence in situ. By combining…
Fluorescent Detection of Cell Senescence
For Flow Cytometry and Imaging Applications
Cellular Senescence is an irreversible growth arrest triggered to prevent DNA damaged cells from dividing. During cellular senescence, senescence-associated beta-galactosidase (SA-beta-gal) activity becomes highly over-expressed, making it a suitable biomarker for identifying senescent…
Cellular Senescence is an irreversible growth arrest triggered to prevent DNA damaged cells from dividing. During cellular senescence, senescence-associated beta-galactosidase (SA-beta-gal) activity becomes highly over-expressed, making it a suitable biomarker for identifying senescent…