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AAT Bioquest

FAQ

Posted on March 9, 2023
What is the procedure of direct stain using a basic dye?
IHCImmunohistochemistry (IHC)Microscopybacteria


Posted on March 9, 2023
What is the procedure for heat fixation?
FixationIHCImmunohistochemistry (IHC)Microscopybacteriafixed cells


Posted on March 9, 2023
What is the principle of direct staining?
IHCImmunohistochemistry (IHC)Microscopybacteria


Posted on May 9, 2020
Is mouse CD16/CD32 a marker for any particular cell type?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)


Posted on May 9, 2020
What markers can I use for investigating microglial activation in flow cytometry?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Flow Cytometry ReagentsIHCImmunohistochemistry (IHC)flow cytometry


Posted on May 9, 2020
What markers can I use for macrophages?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)


Posted on May 9, 2020
What markers can I use for neutrophils?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)


Posted on May 9, 2020
What markers can I use for plasma cells?
BioconjugationAntibody and Protein LabelingAntibody and ProteomicsCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)


Posted on May 9, 2020
What is the effect of phenol red in DMEM (Dulbecco’s Modified Eagle Medium)?
Chemical ReagentsCell CultureIHCImmunohistochemistry (IHC)


Posted on May 9, 2020
Can CD31 also be used as a marker for platelets instead of CD41?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)


Posted on May 9, 2020
How can one distinguish naive from effector/effector memory CD8 T cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)


Posted on May 9, 2020
What is a buffy coat?
DNA extractionIHCImmunohistochemistry (IHC)centrifugefluorescence microscopeimmunofluorescence


Posted on May 9, 2020
What is the best way to store a buffy coat?
Antibodies and ProteomicsDNADNA extractionIHCImmunohistochemistry (IHC)Storage


Posted on May 9, 2020
What are natural killer cells?
Antibodies and ProteomicsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)fluorescence microscopeimmunofluorescence


Posted on May 9, 2020
How do natural killer cells function?
Antibodies and ProteomicsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)


Posted on May 9, 2020
How can I analyze natural killer cells using flow cytometry?
Antibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Flow Cytometry ReagentsIHCImmunohistochemistry (IHC)Spectral Flow Cytometryflow cytometry


Posted on April 24, 2020
Why are there different antibody isotypes in our immune system?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingIHCIgGImmunohistochemistry (IHC)Physiological Probes


Posted on April 24, 2020
Is there a good antibody available to detect C-terminal fragments of amyloid precursor protein (APP)?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingIHCImmunohistochemistry (IHC)


Posted on April 24, 2020
What is the difference between HLA-DR and Iba1?
Antibodies and Proteomics BioconjugationIHCImmunohistochemistry (IHC)Protein


Posted on April 24, 2020
How do you dilute primary antibodies from 1:200 to 1:1000?
Chemical ReagentsBuffer Preparations and RecipesCalculating ConcentrationIHCImmunohistochemistry (IHC)Serial Dilutionbuffer


Posted on April 24, 2020
What is the difference between indirect and direct imaging?
Antibodies and ProteomicsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodiesimmunofluorescence


Posted on April 24, 2020
Why might polyclonal antibodies give better results than monoclonal antibodies?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)Physiological Probes


Posted on April 24, 2020
What is the difference between capture antibody and marker-antigen?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingELISAIHCImmunohistochemistry (IHC)


Posted on April 24, 2020
What is the difference between polyclonal and monoclonal antibodies?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)Protein


Posted on April 17, 2020
Why is 100% Ethanol used in rehydration of paraffin embedded tissues?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)Paraffin embedded sectionsProtein


Posted on April 17, 2020
How does PBS clean cells?
Chemical ReagentsBuffer Preparations and RecipesIHCImmunohistochemistry (IHC)PBSbuffer


Posted on April 17, 2020
What are the advantages and disadvantages of varying incubation time and temperature for immunofluorescence staining of tissue?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)immunofluorescenceIHC


Posted on April 17, 2020
Does adding triton with the primary antibody hinder antigen detection in immunohistochemistry?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)Primary Antibodies Chemical ReagentsBuffer Preparations and RecipesIHCbufferdetergent


Posted on April 17, 2020
What is the reason for positive staining in negative control tissue during Immunohistochemistry (IHC)?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)IHChydrogen peroxide


Posted on April 17, 2020
What is IHC double staining?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)Chemical ReagentsClassic DyesIHCPhysiological Probes


Posted on April 17, 2020
What is the detailed mechanism of PMA differentiation of U937 cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingIHCImmunohistochemistry (IHC)


Posted on April 17, 2020
Why is the function of E-Cadherin totally different from N-Cadherin?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingIHCImmunohistochemistry (IHC)


Posted on April 17, 2020
What is blocking buffer in IHC?
Antibodies and ProteomicsBSAImmunohistochemistry (IHC)PBSbuffer Chemical ReagentsIHC


Posted on April 17, 2020
What is the role of Tween-20 in antigen retrieval buffer?
Immunohistochemistry (IHC)detergent Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesIHCbuffer


Posted on April 17, 2020
What are some reliable methods for validating antibodies?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingIHCImmunohistochemistry (IHC)Western Blot


Posted on April 17, 2020
Is agitation necessary during IHC antibody incubation?
Antibodies and ProteomicsBuffer Preparations and RecipesIHCImmunohistochemistry (IHC)Permeabilizationbufferdetergent


Posted on April 2, 2020
What is CD45 a marker for?
Antibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)IHC


Posted on April 2, 2020
What is the role of CD45 in T cell activation?
Antibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)IHC


Posted on April 2, 2020
What is the difference between CD4 and CD8 T cells?
Antibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)IHC


Posted on April 2, 2020
What are the roles of CD4 and CD8 in T cell activation?
Antibodies and ProteomicsAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)


Posted on March 10, 2020
What is Tyramide Signal Amplification (TSA)?
Tyramide Signal AmplificationTSAIHCICCFISHin situ hybridizationimmunohistochemistryimmunocytochemistry


Posted on February 25, 2020
What is IHC?
immunohistochemistryIHC


Posted on December 6, 2019
How do you activate T cells?
Cell AssayELISAIHCT cellantibody labelingmicroplate


Posted on December 6, 2019
What cd80 86?
IHCT cellantibody labelingimmunohistochemistry


Posted on August 26, 2019
Why are cells lysed?
Cell AssayCell ViabilityWestern Blotcell lysishomogenouscentrifugeIHCSignal Amplificationantibody labelingimmunohistochemistry