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AAT Bioquest

FAQ

Posted on February 19, 2020
My NAD+ assay is giving inconsistent results when repeated-do you have any suggestions as to why?
AmpliteNADNAD/NADH RatioNADHNADPNADP/NADPH ratioNADPHcell linesenzymenicotinamide adenine dinucleotidenicotinamide adenine dinucleotide phosphateNADH Assay


Posted on February 19, 2020
Why is my NADH assay showing unreasonably high absorbance readings?
AbsorbanceCell AssayColorimetricNADH AssayNADNADHNADPNADPHStock Solutionbufferenzymenicotinamide adenine dinucleotidenicotinamide adenine dinucleotide phosphatespectrophotometer


Posted on October 7, 2019
Why is the sum of my NAD and NADH extraction readings greater than my Total NAD/NADH reading?
nicotinamide adenine dinucleotideNADNADHNADH AssayNAD/NADH Ratio


Posted on August 26, 2019
Are NADH and ROS related?
Cell ViabilityNADNADHNAD/NADH RatioNADPNADPHNADP/NADPH ratioROSROS ProbesReactive Oxygen SpeciesTotal ROS probecell damagemitochondria ROS


Posted on August 26, 2019
What does NAD+ do in the body?
Cell ViabilityNAD/NADH RatioNADNADHNADP/NADPH ratioNADPHNADPNADH Assayhuman


Posted on August 21, 2019
Can I measure NADPH without lysing my cells?
NADH AssayNADP/NADPH ratioNADHNADPNADPHNADWestern Blotcell lysiscell assayemission wavelengthexcitation wavelengthfluorescence imagingfluorimetriclive cell analysislive cell assaymultiplex


Posted on June 13, 2019
What is the best way to quantitate Cyclic ADP-Ribose (cADPR)?
NADCa2+calciumcell assayfluorimetriccalcium mobilitysecond messengerfluorescent plate reader


Posted on June 13, 2019
What assay kits measure NAD/NADH from cell samples?
NADNADHNAD/NADH Ratio


Posted on September 19, 2018
How do I prepare/lyse my cell samples for Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence*?
NADNADHNAD/NADH RatioNADH Assaynicotinamide adenine dinucleotide


Posted on September 19, 2018
Will Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* work with NADP/NADPH? Can this kit measure NADP+ and NADPH?
NADNADHNADPNADPH standardNAD/NADH RatioNADP/NADPH ratioNADNADHNADH AssayNAD/NADH Rationicotinamide adenine dinucleotide


Posted on September 19, 2018
Can Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* be used for plant cells? for bacterial cells? for mammalian cells? for tissue samples?
NADNADHNADH AssayNAD/NADH Rationicotinamide adenine dinucleotide


Posted on August 22, 2018
Is Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* pH sensitive? Do I have to prepare my samples in a certain pH buffer? Will pH influence my fluorescence readings?
nicotinamide adenine dinucleotidenicotinamide adenine dinucleotide phosphateNADNADHNADH AssayNAD/NADH Ratio


Posted on August 21, 2018
Can Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* be used to quantify NAD and NADH separately? Can this assay measure only NAD? Can this assay measure only NADH?
nicotinamide adenine dinucleotidenicotinamide adenine dinucleotide phosphateNADNADHNAD/NADH RatioNADH Assay


Posted on August 20, 2018
What is a typical NAD to NADH ratio?
nicotinamide adenine dinucleotideNADnicotinamide adenine dinucleotide phosphateNADPNADHNAD/NADH Ratio


Posted on July 9, 2018
Will reducing agents such as Trolox, DTT and TCEP cause any interference when using your fluorimetric total NAD and NADH assay (Cat# 15257)?
NADNADH Assay