How do you stain paraffin-embedded tissue with a phalloidin"conjugate?

Reagents

Fixative - 3.7% paraformaldehyde in PBS
Permeabilization Buffer – PBS+ 0.2% Triton X-100
Blocking Buffer – PBS+1% BSA+0.05% Triton X-100

Procedure:

1. Dewax the tissue.
2. Add Permeabilization Buffer for one or two hours, slowly rotate the tissue sections .
3. After Permeabilization, wash the tissue sections with three changes of PBS+ 0.2% Triton X-100 Buffer, 5-10 min/wash.
4. Add Blocking Buffer to block the nonspecific fluorophore binding sites for 1-2 hours.
5. After blocking, place the slides in a humidity chamber and add 1X Phalloidin–Conjugate in PBS Buffer + 1% BSA  to the surface of the tissue section. Incubate the covered slides in the humidity chamber for one or two hours at RT or 37^oC, avoid light.
6. Wash with excess PBS+ 0.2% Triton X-100.
7. Observe under microscope.