Web Tools
AAT Bioquest offers a number of web tools to aid researchers in planning, developing and running experiments. Below is a list of our currently available tools.


Interactive Spectrum Viewer
Allows users to view and compare spectral (excitation and emission) data. This tool is essential for experimental planning and choosing the right fluorophore/label.

Extinction Coefficient Finder
Search a large database of fluorescent compounds. Extinction coefficient is an important factor in quantifying probe "brightness" and is useful for calculations of concentration and degree of labeling.

Concentration & Molecular Weight
DNA molecular weight calculator - When performing a PCR or gel run, the molecular weight of a sense strand of RNA can be important to know. This calculator converts a string of nucleotides into a molecular weight value. Additionally, the antisense strand of the DNA is provided, along with its own molecular weight.

Peptide and protein molecular weight calculator - Amino acids, the constituent organic compounds making up peptides and proteins, form long chains that determine shape and function. This calculator provides the molecular weight of a peptide or protein based on a string of amino acids given.

Protein concentration calculator - For many assays, it is critical to know the concentration of a protein solution prior to experimentation. The concentration of a protein solution can be determined using its absorbance value at its maximum absorbance wavelength and a derived form of the Beer-Lambert law.

RNA molecular weight calculator - When performing a PCR or gel run, the molecular weight of a sense strand of RNA can be important to know. This calculator converts a string of nucleotides into a molecular weight value. Additionally, the antisense strand of the DNA is provided, along with its own molecular weight.


Data Analysis & Regression
4PL calculator - Fits a symmetrical sigmoidal, "s", shaped curve for experimental data. Generates equation and graph.

EC50 calculator - In pharmacology, the EC50 represents the concentration of a substance which results in half of its maximal response. This calculator will determine the EC50 given experimental data.

IC50 calculator - The IC50 of an antagonist is the concentration at which half of the inhibitory effect is achieved. It is an important value in pharmacology as it characterizes a drug's potency on biological processes. This calculator determines the IC50 given experimental data.

Linear, Logarithmic, Semi-Log Regression Calculator - This calculator determines a best fit linear function using the least-squares method. It will provide four different linear regressions: linear-linear, log-linear, linear-log and log-log.


Experiment Planning
Buffer preparations - A list of common physiological buffers used in research. Includes buffers such as acetate buffer, HBSS (Hanks' balanced salt solution) and PBS. Recipe is automatically calculated based on desired volume. pH and molarity can be calculated automatically for acid-base derived buffers.

Serial dilution calculator - Serial dilutions are a common step in biological research, crafting a specially-tuned solution for use in later steps of an experiment. The serial dilution calculator generates a series of instructions to guide you through the dilution of any solution, given an initial concentration and dilution factor, or a concentration range.

Spectral Data
Extinction coefficient finder - The extinction coefficient represents the degree to which a compound attenuates light at a specific wavelength. Often, the specific wavelength of interest corresponds to the compound's maximal absorbance. Together with quantum yield, the extinction coefficient is an important parameter in a compound's "brightness".

Spectrum viewer - An important step in experimental planning is choosing the right fluorophore. The spectrum viewer web application allows user to compare a vast library of fluorophores based on excitation and emission values. Explore which fluorophores are compatible with a particular instrument, laser, or filter set. Plan multiplex assays by choosing spectra with minimal overlap. Or choose spectra with optimal overlap for developing tandem and FRET based labels.


Other Tools
Degree of labeling calculator - One of the most important metrics in bioconjugation is the degree of labeling (DOL). This value represents the number of labels attached to each target (ie. protein). An optimal DOL is critical to the success of a conjugation. DOL values that are too high or too low tend to yield poor performing conjugates. This tool helps calculate the DOL of common label-target pairs. Requires absorbance values from a spectrophotometer or similar instrument.