AAT Bioquest

New Products	Featured Products	AssayWise Newsletters
Amplite™ Fluorimetric Maleimide Quantitation Kit There are few reagents or assay kits available for quantitate the number of maleimide groups that are introduced into a protein or other macromolecules. All the commercial kits have tedious protocols. Our kit uses a proprietary dye that has enhanced fluorescence upon reacting with a maleimide. The kit provides a sensitive, one-step fluorimetric method to detect as little as 10 picomoles of maleimide in a 100 µL assay volume (100 nM in concentration). Amplite™ Fluorimetric Thiol Quantitation Assay Kit Our Amplite™ Fluorimetric Thiol Qutitation Kit provides an ultrasensitive fluorimetric assay for quantitating thiols that exist either in a small molecule or on a protein. The kit uses a proprietary non-fluorescent dye that becomes strongly fluorescent upon reacting with thiol. The kit provides a sensitive, one-step fluorimetric method to detect as little as 1 picomole of cysteine or GSH in a 100 µL assay volume (10 nM in concentration). The assay is rapid and robust. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with Ex/Em = 490 nm/520 nm.	Quest Fluo-8™ Quest Fluo-8 calcium indicators and Screen Quest™ Fluo-8 Calcium Assay Kit provide a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. Fluo-8 is the brightest green calcium indicator available for HTS screening. It has 100-250 times fluorescence increases when it forms complexes with calcium. The Screen Quest Fluo-8 NW Calcium Assay Kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. Quest Rhod-4™ Rhod-2 is most commonly used among the red fluorescent calcium indicators. However, Rhod-2 AM is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses. Quest Rhod-4™ has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2. In CHO and HEK cells Quest Rhod-4™ AM has cellular calcium response that is 10 times more sensitive than Rhod-2 AM.	AssayWise Newsletters is a quarterly publication of AAT Bioquest. It highlights the newest assay technologies developed by AAT Bioquest and its collaborators. The newsletter is divided into five sections including: 1) Biochemical assays 2) Cell-based assays 3) High content analysis 4) HTS assays 5) Labeling probes We welcome your contribution that uses AAT Bioquest’s products or services. Your valuable suggestions are highly appreciated. We would take every effort to provide you the best products possible to help you discover more and discover better. Please request your copy by e-mailing us at support@aatbio.com or call us at 408-733-1055.