6-FAM [6-Carboxyfluorescein] CAS 3301-79-9

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Physical properties

Molecular weight	376.32
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

3301-79-9

Molecular weight

376.32

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.178

Extinction coefficient (cm ^-1 M ^-1)

83000

Excitation (nm)

493

Emission (nm)

517

6-FAM is the other purified isomer of 5(6)-carboxyfluorescein. Complimemtary to 5-FAM isomer, 6-FAM is mainly used in labeling nucleotides and nucleic acids.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-FAM [6-Carboxyfluorescein] *CAS 3301-79-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	265.731 µL	1.329 mL	2.657 mL	13.287 mL	26.573 mL
5 mM	53.146 µL	265.731 µL	531.463 µL	2.657 mL	5.315 mL
10 mM	26.573 µL	132.866 µL	265.731 µL	1.329 mL	2.657 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
5-FAM [5-Carboxyfluorescein] CAS 76823-03-5	493	517	83000	0.178
6-DTAF [6-(4,6-Dichlorotriazinyl)aminofluorescein] CAS 118357-32-7	498	517	80000¹	0.35
6-CR110 [6-Carboxyrhodamine 110] Single isomer	499	525	84000	0.091
6-CR6G [6-Carboxyrhodamine 6G] Single isomer	522	546	94000	0.214
6-TAMRA [6-Carboxytetramethylrhodamine] CAS 91809-67-5	552	578	90000	0.178
6-ROX [6-Carboxy-X-rhodamine] CAS 194785-18-7	578	604	82000	0.168
6-TRITC [Tetramethylrhodamine-6-isothiocyanate] CAS 80724-20-5	544	570	100000	0.34

Images

Figure 1. Chemical structure for 6-FAM [6-Carboxyfluorescein] *CAS 3301-79-9*

Citations

View all 5 citations: Citation Explorer

A natural in situ fabrication method of functional bacterial cellulose using a microorganism
Authors: Gao, Minghong and Li, Juan and Bao, Zixian and Hu, Moudong and Nian, Rui and Feng, Dexin and An, Dong and Li, Xing and Xian, Mo and Zhang, Haibo
Journal: Nature Communications (2019): 437

Multicellular Vascularized Engineered Tissues through User-Programmable Biomaterial Photodegradation
Authors: Arakawa, Christopher K and Badeau, Barry A and Zheng, Ying and DeForest, Cole A
Journal: Advanced Materials (2017)

Green tea inhibited the elimination of nephro-cardiovascular toxins and deteriorated the renal function in rats with renal failure
Authors: Peng, Yu-Hsuan and Sweet, Douglas H and Lin, Shiuan-Pey and Yu, Chung-Ping and Lee Chao, Pei-Dawn and Hou, Yu-Chi
Journal: Scientific Reports (2015): 1--10

Green tea inhibited the elimination of nephro-cardiovascular toxins and deteriorated the renal function in rats with renal failure
Authors: Peng, Yu-Hsuan and Sweet, Douglas H and Lin, Shiuan-Pey and Yu, Chung-Ping and Chao, Pei-Dawn Lee and Hou, Yu-Chi
Journal: Scientific reports (2015)

Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Preus, Søren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001

References

View all 137 references: Citation Explorer

Carboxyfluorescein leakage from poly(ethylene glycol)-grafted liposomes induced by the interaction with serum
Authors: Hashizaki K, Taguchi H, Sakai H, Abe M, Saito Y, Ogawa N.
Journal: Chem Pharm Bull (Tokyo) (2006): 80

Quantifying lymphocyte kinetics in vivo using carboxyfluorescein diacetate succinimidyl ester (cfse)
Authors: Asquith B, Debacq C, Florins A, Gillet N, Sanchez-Alcaraz T, Mosley A, Willems L.
Journal: Proc Biol Sci (2006): 1165

Application of alamar blue/5-carboxyfluorescein diacetate acetoxymethyl ester as a noninvasive cell viability assay in primary hepatocytes from rainbow trout
Authors: Schreer A, Tinson C, Sherry JP, Schirmer K.
Journal: Anal Biochem (2005): 76

Low incidence of acute rejection after living-donor liver transplantation: Immunologic analyses by mixed lymphocyte reaction using a carboxyfluorescein diacetate succinimidyl ester labeling technique
Authors: Tanaka Y, Ohdan H, Onoe T, Mitsuta H, Tashiro H, Itamoto T, Asahara T.
Journal: Transplantation (2005): 1262

Evidence for dimer formation by an amphiphilic heptapeptide that mediates chloride and carboxyfluorescein release from liposomes
Authors: Pajewski R, Ferdani R, Pajewska J, Djedovic N, Schlesinger PH, Gokel GW.
Journal: Org Biomol Chem (2005): 619

Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling
Authors: Wang XQ, Duan XM, Liu LH, Fang YQ, Tan Y.
Journal: Acta Biochim Biophys Sin (Shanghai) (2005): 379

Application of glutaraldehyde for the staining of esterase-active cells with carboxyfluorescein diacetate
Authors: Morono Y, Takano S, Miyanaga K, Tanji Y, Unno H, Hori K.
Journal: Biotechnol Lett (2004): 379

Drug efflux transport properties of 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (bcecf-am) and its fluorescent free acid, bcecf
Authors: Bachmeier CJ, Trickler WJ, Miller DW.
Journal: J Pharm Sci (2004): 932

pruning of alloreactive cd4+ t cells using 5- (and 6-)carboxyfluorescein diacetate succinimidyl ester prolongs skin allograft survival
Authors: Watson D, Zhang GY, Sartor M, Alex and er SI., undefined
Journal: J Immunol (2004): 6574

Cyclodextrin enhanced transdermal delivery of piroxicam and carboxyfluorescein by electroporation
Authors: Murthy SN, Zhao YL, Sen A, Hui SW.
Journal: J Control Release (2004): 393