Calculator
CF-MUP, sodium salt *Superior alternative to MUP*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 352.55 |
| Solvent | Water |
Spectral properties
| Excitation (nm) | 356 |
| Emission (nm) | 456 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
Molecular weight 352.55 | Excitation (nm) 356 | Emission (nm) 456 |
Although MUP is widely used for detecting phosphatases in solution it is not well suited for living cell or continuous assays since MU (4-methylumbelliferone), the enzymatic product, which only develops maximum fluorescence at pH value of >10. Thus it is also difficult to use MUP for the detection of phosphatases that have acidic optimal pH range such as acid phosphatases. AAT Bioquest is pleased to offer CF-MUP Plus that is developed to address this pH limitation associated with MUP substrates. CF-MU exhibits maximum fluorescence above pH 5.0, thus CF-MUP substrate can be well used for continuous phosphatase assays. It can also be used for the assays that require acidic pH such as acid phosphatases and some protein phosphatases.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of CF-MUP, sodium salt *Superior alternative to MUP* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 283.648 µL | 1.418 mL | 2.836 mL | 14.182 mL | 28.365 mL |
| 5 mM | 56.73 µL | 283.648 µL | 567.295 µL | 2.836 mL | 5.673 mL |
| 10 mM | 28.365 µL | 141.824 µL | 283.648 µL | 1.418 mL | 2.836 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Images
Figure 1. Detection of acid phosphatase activity CF-MUP and MUP. The concentration of the two substrates (initially approximately 10 uM) were matched by normalizing the obsorbance at 319 nm (pH = 10) to a value of 0.52 (assuming the extinction coefficient of each substrate was approximately equivalent). The resulting fluorescence signal was recorded using Ex/Em = 360/450 nm.
References
View all 76 references: Citation Explorer
Application of intracellular alkaline phosphatase activity measurement in detection of neutrophil adherence in vitro
Authors: Bednarska K, Klink M, Sulowska Z.
Journal: Mediators Inflamm (2006): 19307
Authors: Bednarska K, Klink M, Sulowska Z.
Journal: Mediators Inflamm (2006): 19307
Evaluation of an alternative method for the enumeration and confirmation of Clostridium perfringens from treated and untreated sewages
Authors: Wohlsen T, Bayliss J, Gray B, Bates J, Katouli M.
Journal: Lett Appl Microbiol (2006): 438
Authors: Wohlsen T, Bayliss J, Gray B, Bates J, Katouli M.
Journal: Lett Appl Microbiol (2006): 438
Fluorometric cell-ELISA for quantifying rabies infection and heparin inhibition
Authors: Rincon V, Corredor A, Martinez-Gutierrez M, Castellanos JE.
Journal: J Virol Methods (2005): 33
Authors: Rincon V, Corredor A, Martinez-Gutierrez M, Castellanos JE.
Journal: J Virol Methods (2005): 33
Relative movement and soil fixation of soluble organic and inorganic phosphorus
Authors: Anderson BH, Magdoff FR.
Journal: J Environ Qual (2005): 2228
Authors: Anderson BH, Magdoff FR.
Journal: J Environ Qual (2005): 2228
Purification and partial characterization of an acid phosphatase from Spirodela oligorrhiza and its affinity for selected organophosphate pesticides
Authors: Hoehamer CF, Mazur CS, Wolfe NL.
Journal: J Agric Food Chem (2005): 90
Authors: Hoehamer CF, Mazur CS, Wolfe NL.
Journal: J Agric Food Chem (2005): 90
Crystal structure of a covalent intermediate of endogenous human arylsulfatase A
Authors: Chruszcz M, Laidler P, Monkiewicz M, Ortlund E, Lebioda L, Lewinski K.
Journal: J Inorg Biochem (2003): 386
Authors: Chruszcz M, Laidler P, Monkiewicz M, Ortlund E, Lebioda L, Lewinski K.
Journal: J Inorg Biochem (2003): 386
Enzymatic differentiation of Candida parapsilosis from other Candida spp. in a membrane filtration test
Authors: Bauters TG, Peleman R, Dhont M, Vanhaesebrouck P, Nelis HJ.
Journal: J Microbiol Methods (2003): 11
Authors: Bauters TG, Peleman R, Dhont M, Vanhaesebrouck P, Nelis HJ.
Journal: J Microbiol Methods (2003): 11
Development of a rapid 1-h fluorescence-based cytotoxicity assay for Listeria species
Authors: Shroyer ML, Bhunia AK.
Journal: J Microbiol Methods (2003): 35
Authors: Shroyer ML, Bhunia AK.
Journal: J Microbiol Methods (2003): 35
LVV-hemorphin-4 modulates Ca2+/calmodulin-dependent pathways in the immune system by the same mechanism as in the brain
Authors: Barkhudaryan N, Gambarov S, Gyulbayazyan T, Nahapetyan K.
Journal: J Mol Neurosci (2002): 203
Authors: Barkhudaryan N, Gambarov S, Gyulbayazyan T, Nahapetyan K.
Journal: J Mol Neurosci (2002): 203
A colorimetric and fluorometric microplate assay for the detection of microcystin-LR in drinking water without preconcentration
Authors: Bouaicha N, Maatouk I, Vincent G, Levi Y.
Journal: Food Chem Toxicol (2002): 1677
Authors: Bouaicha N, Maatouk I, Vincent G, Levi Y.
Journal: Food Chem Toxicol (2002): 1677