Resorufin Phosphocholine

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Physical properties

Molecular weight	378.32
Solvent	Water

Spectral properties

Absorbance (nm)	570
Extinction coefficient (cm ^-1 M ^-1)	65000¹
Excitation (nm)	571
Emission (nm)	584
Quantum yield	0.75¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

378.32

Absorbance (nm)

570

Extinction coefficient (cm ^-1 M ^-1)

65000¹

Excitation (nm)

571

Emission (nm)

584

Quantum yield

0.75¹

Glycerophosphocholine phosphodiesterase release phosphocholine from resorufin phosphocholine to give the highly fluorescent resorufin that can be readily monitored by either fluorescence or absorption. This fluorogenic phosphodiesterase substrate can be conveniently used for screening the inhibitors of glycerophosphocholine phosphodiesterase. It can also be used for monitoring the activities of phospholipase D in combination with a phosphatase. Phospholipase D hydrolyzes resorufin phosphocholine to resorufin phosphate, which in the presence of an acid phosphatase generates resorufin. Thus, resorufin phosphocholine can also be used for monitoring phospholipase D in sources that do not also contain high levels of phospholipase C.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Resorufin Phosphocholine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	264.326 µL	1.322 mL	2.643 mL	13.216 mL	26.433 mL
5 mM	52.865 µL	264.326 µL	528.653 µL	2.643 mL	5.287 mL
10 mM	26.433 µL	132.163 µL	264.326 µL	1.322 mL	2.643 mL

Molarity calculator

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Spectrum

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Spectral properties

Absorbance (nm)	570
Extinction coefficient (cm ^-1 M ^-1)	65000¹
Excitation (nm)	571
Emission (nm)	584
Quantum yield	0.75¹

Images

Figure 1. Chemical structure for Resorufin Phosphocholine

References

View all 81 references: Citation Explorer

Identification of phosphotyrosine mimetic inhibitors of human tyrosyl-DNA phosphodiesterase I by a novel AlphaScreen high-throughput assay
Authors: March, undefined and C, Lea WA, Jadhav A, Dexheimer TS, Austin CP, Inglese J, Pommier Y, Simeonov A.
Journal: Mol Cancer Ther (2009): 240

The effects of a type 4 phosphodiesterase inhibitor and the muscarinic cholinergic antagonist tolterodine tartrate on detrusor overactivity in female rats with bladder outlet obstruction
Authors: Kaiho Y, Nishiguchi J, Kwon DD, Chancellor MB, Arai Y, Snyder PB, Yoshimura N.
Journal: BJU Int (2008): 615

Antiplatelet cilostazol, an inhibitor of type III phosphodiesterase, improves swallowing function in patients with a history of stroke
Authors: Teramoto S, Yamamoto H, Yamaguchi Y, Ishii M, Hibi S, Kume H, Ouchi Y.
Journal: J Am Geriatr Soc (2008): 1153

Inhibition of platelet aggregation by olive oil phenols via cAMP-phosphodiesterase
Authors: Dell'Agli M, Maschi O, Galli GV, Fagnani R, Dal Cero E, Caruso D, Bosisio E.
Journal: Br J Nutr (2008): 945

Lipolytic effect of a polyphenolic citrus dry extract of red orange, grapefruit, orange (SINETROL) in human body fat adipocytes. Mechanism of action by inhibition of cAMP-phosphodiesterase (PDE)
Authors: Dallas C, Gerbi A, Tenca G, Juchaux F, Bernard FX.
Journal: Phytomedicine (2008): 783

Selective inhibitors for phosphodiesterase 3 and 4 in antigen-induced increase of cough reflex sensitivity in guinea pigs
Authors: Fujimura M, Liu Q.
Journal: Pulm Pharmacol Ther (2007): 543

Characterization of the 2',3' cyclic phosphodiesterase activities of Clostridium thermocellum polynucleotide kinase-phosphatase and bacteriophage lambda phosphatase
Authors: Keppetipola N, Shuman S.
Journal: Nucleic Acids Res (2007): 7721

Biochemical characterization of ecto-nucleotide pyrophosphatase/phosphodiesterase (E-NPP, E.C. 3.1.4.1) from rat heart left ventricle
Authors: Rucker B, Almeida ME, Libermann TA, Zerbini LF, Wink MR, Sarkis JJ.
Journal: Mol Cell Biochem (2007): 247

Characterization of a phosphodiesterase capable of hydrolyzing EA 2192, the most toxic degradation product of the nerve agent VX
Authors: Ghanem E, Li Y, Xu C, Raushel FM.
Journal: Biochemistry (2007): 9032

Developmental exposure to estradiol and bisphenol A increases susceptibility to prostate carcinogenesis and epigenetically regulates phosphodiesterase type 4 variant 4
Authors: Ho SM, Tang WY, Belmonte de Frausto J, Prins GS.
Journal: Cancer Res (2006): 5624