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D-Luciferin galactoside *CAS 131474-38-9*

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Physical properties
Molecular weight442.46
SolventDMSO
Spectral properties
Excitation (nm)385
Emission (nm)529
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41105331

OverviewpdfSDSpdfProtocol


CAS
131474-38-9
Molecular weight
442.46
Excitation (nm)
385
Emission (nm)
529
D-Luciferin galactoside contains a beta-galactoside moiety attached at the 6-O-position, thus preventing it be recognized by the firefly luciferase enzyme. However, the galactose moiety is removed effectively by beta-galactosidase activity, and the resulted D-luciferin is well recognized by luciferase. D-Luciferin galactoside is a sensitive substrate for the chemiluminescent measurement of galactosidase activity in homogeneous assays, or in cell lysate samples in combination with luciferase and its cofactors. When used as a dual substrate, beta-galactosidase (LacZ gene) and luciferase levels can be determined with high sensitivity. When used in a model system expressing firefly luciferase, the luciferin is then utilized in a firefly luciferase reaction to generate light. It can also be used for in vivo bioluminescence imaging applications.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of D-Luciferin galactoside *CAS 131474-38-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM226.009 µL1.13 mL2.26 mL11.3 mL22.601 mL
5 mM45.202 µL226.009 µL452.018 µL2.26 mL4.52 mL
10 mM22.601 µL113.005 µL226.009 µL1.13 mL2.26 mL

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Spectrum


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Spectral properties

Excitation (nm)385
Emission (nm)529

Product Family


NameExcitation (nm)Emission (nm)
D-Luciferin phosphate *CAS 145613-12-3*385529
D-Luciferin acetate385529

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Citations


View all 4 citations: Citation Explorer
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Journal: Plant Physiology (2023): kiad445
Design, synthesis, and biological evaluation studies of novel carboxylesterase 2 inhibitors for the treatment of irinotecan-induced delayed diarrhea
Authors: Yang, Zhongcheng and Cao, Zhijun and Wang, Wenxin and Chen, Ya and Huang, Wanqiu and Jiao, Shixuan and Chen, Siliang and Chen, Lianru and Liu, Yuxia and Mao, Jianming and others,
Journal: Bioorganic Chemistry (2023): 106625
Diltiazem inhibits breast cancer metastasis via mediating growth differentiation factor 15 and epithelial-mesenchymal transition
Authors: Chen, Yen-Chang and Wu, Chen-Teng and Chen, Jia-Hong and Tsai, Cheng-Fang and Wu, Chen-Yun and Chang, Pei-Chun and Yeh, Wei-Lan
Journal: Oncogenesis (2022): 1--9
Identification of a novel protein kinase a inhibitor by bioluminescence-based screening
Authors: Ishimoto, Tetsuya and Azechi, Kenji and Mori, Hisashi
Journal: Biological and Pharmaceutical Bulletin (2015): 1969--1974

References


View all 3 references: Citation Explorer
Luminescent imaging of beta-galactosidase activity in living subjects using sequential reporter-enzyme luminescence
Authors: Wehrman TS, von Degenfeld G, Krutzik PO, Nolan GP, Blau HM.
Journal: Nat Methods (2006): 295
Homogeneous enzyme immunoassay modified for application to luminescence-based biosensors
Authors: Yang X, Janatova J, Andrade JD.
Journal: Anal Biochem (2005): 102
Development of a rapid positive/absent test for coliforms using sensitive bioluminescence assay
Authors: Masuda-Nishimura I, Fukuda S, Sano A, Kasai K, Tatsumi H.
Journal: Lett Appl Microbiol (2000): 130