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FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK

Fluorometric detection of active caspases 3/7 using FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK (Cat# 13418) in Jurkat cells. The cells were treated with 1 μM staurosporine for 4 hours (Green) while untreated cells were used as a control (Red). Control or treated Cells were incubated with FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK for 1 hour at 37 °C, and then washed once after stain.  Fluorescent intensity was measured with NovoCyte™ 3000 Flow Cytometer FITC channel.
Fluorometric detection of active caspases 3/7 using FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK (Cat# 13418) in Jurkat cells. The cells were treated with 1 μM staurosporine for 4 hours (Green) while untreated cells were used as a control (Red). Control or treated Cells were incubated with FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK for 1 hour at 37 °C, and then washed once after stain.  Fluorescent intensity was measured with NovoCyte™ 3000 Flow Cytometer FITC channel.
Fluorometric detection of active caspases 3/7 using FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK (Cat# 13418) in Jurkat cells. The cells were treated with 1 μM staurosporine for 4 hours (Green) while untreated cells were used as a control (Red). Control or treated Cells were incubated with FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK for 1 hour at 37 °C, and then washed once after stain.  Fluorescent intensity was measured with NovoCyte™ 3000 Flow Cytometer FITC channel.
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Physical properties
Molecular weight1037.07
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)73000
Excitation (nm)491
Emission (nm)516
Quantum yield0.92
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
1037.07
Correction Factor (280 nm)
0.35
Extinction coefficient (cm -1 M -1)
73000
Excitation (nm)
491
Emission (nm)
516
Quantum yield
0.92
Activation of caspases plays a central role in apoptosis. FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK provides a convenient means for sensitive detection of activated caspase-3 in living cells. FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells. Based on our in-house studies FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK gave the highest signal/background ratio compared to other DEVD-FMK probes we tested. The FITC label allows for direct detection of activated caspases in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM96.426 µL482.128 µL964.255 µL4.821 mL9.643 mL
5 mM19.285 µL96.426 µL192.851 µL964.255 µL1.929 mL
10 mM9.643 µL48.213 µL96.426 µL482.128 µL964.255 µL

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Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)73000
Excitation (nm)491
Emission (nm)516
Quantum yield0.92

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References


View all 4 references: Citation Explorer
Activation of caspase-like activity and poly (ADP-ribose) polymerase degradation during sporulation in Aspergillus nidulans
Authors: Thrane C, Kaufmann U, Stummann BM, Olsson S.
Journal: Fungal Genet Biol (2004): 361
Antitumor mechanism of 3-bromopropionylamino benzoylurea on leukemia and lymphoma
Authors: Li JN, Song DQ, Jiang JD.
Journal: Yao Xue Xue Bao (2004): 491
Caspase-dependent and independent cell death in rat hepatoma 5123tc cells
Authors: P, undefined and ey S, Smith B, Walker PR, Sikorska M.
Journal: Apoptosis (2000): 265
C-myc antisense oligodeoxynucleotides can induce apoptosis and down-regulate Fas expression in rheumatoid synoviocytes
Authors: Hashiramoto A, Sano H, Maekawa T, Kawahito Y, Kimura S, Kusaka Y, Wilder RL, Kato H, Kondo M, Nakajima H.
Journal: Arthritis Rheum (1999): 954