FDGlcU [Fluorescein di-beta-D-glucuronide]
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 684.55 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 487 |
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 800001 |
Excitation (nm) | 498 |
Emission (nm) | 517 |
Quantum yield | 0.79001, 0.952 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
See also: Rhodamines and Rhodamine Derivatives, Fluoresceins
Molecular weight 684.55 | Absorbance (nm) 487 | Correction Factor (260 nm) 0.32 | Correction Factor (280 nm) 0.35 | Extinction coefficient (cm -1 M -1) 800001 | Excitation (nm) 498 | Emission (nm) 517 | Quantum yield 0.79001, 0.952 |
The beta-glucuronidase (GUS) enzyme from E. coli (EC 3.2.1.31) has been well documented to provide desirable characteristics as a marker gene in transformed plants. The GUS reporter gene system has many advantages including stable expression of E. coli GUS enzyme, no interference with normal plant metabolism, and low intrinsic GUS activity in higher plants. FDGlcU is considered to be one of the most sensitive fluorogenic substrates available for detecting beta-glucuronidase. The colorless and nonfluorescent FDGlcU is hydrolyzed to highly fluorescent fluorescein, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments. Glucuronidase-catalyzed hydrolysis of FDGlcU can be followed by fluorescence increase around 520 nm. Alternatively, FDGlcU can also be used to detect glucuronidase in a chromogenic mode since the enzymatic product (fluorescein) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1). FDGlcU has been used for identifying GUS-positive cells with fluorescence microscopy and flow cytometry.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of FDGlcU [Fluorescein di-beta-D-glucuronide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 146.081 µL | 730.407 µL | 1.461 mL | 7.304 mL | 14.608 mL |
5 mM | 29.216 µL | 146.081 µL | 292.163 µL | 1.461 mL | 2.922 mL |
10 mM | 14.608 µL | 73.041 µL | 146.081 µL | 730.407 µL | 1.461 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Absorbance (nm) | 487 |
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 800001 |
Excitation (nm) | 498 |
Emission (nm) | 517 |
Quantum yield | 0.79001, 0.952 |
References
View all 80 references: Citation Explorer
Active site tyrosine is essential for amidohydrolase but not for esterase activity of a class 2 histone deacetylase-like bacterial enzyme
Authors: Moreth K, Riester D, Hildmann C, Hempel R, Wegener D, Schober A, Schwienhorst A.
Journal: Biochem J. (2006)
Authors: Moreth K, Riester D, Hildmann C, Hempel R, Wegener D, Schober A, Schwienhorst A.
Journal: Biochem J. (2006)
Histone deacetylase inhibitor FR901228 enhances the antitumor effect of telomerase-specific replication-selective adenoviral agent OBP-301 in human lung cancer cells
Authors: Watanabe T, Hioki M, Fujiwara T, Nishizaki M, Kagawa S, Taki M, Kishimoto H, Endo Y, Urata Y, Tanaka N.
Journal: Exp Cell Res (2006): 256
Authors: Watanabe T, Hioki M, Fujiwara T, Nishizaki M, Kagawa S, Taki M, Kishimoto H, Endo Y, Urata Y, Tanaka N.
Journal: Exp Cell Res (2006): 256
Induction of apoptosis and inhibition of telomerase activity by trichostatin A, a histone deacetylase inhibitor, in human leukemic U937 cells
Authors: Woo HJ, Lee SJ, Choi BT, Park YM, Choi YH.
Journal: Exp Mol Pathol. (2006)
Authors: Woo HJ, Lee SJ, Choi BT, Park YM, Choi YH.
Journal: Exp Mol Pathol. (2006)
Enhanced transgene expression in urothelial cancer gene therapy with histone deacetylase inhibitor Okegawa T, Nutahara K, Pong RC, Higashihara E, Hsieh JT. Department of Urology, University of Kyorin, Tokyo, Japan
Authors: Hsieh JT., undefined
Journal: Urol Oncol (2006): 565
Authors: Hsieh JT., undefined
Journal: Urol Oncol (2006): 565
Fetal hemoglobin induction by histone deacetylase inhibitors involves generation of reactive oxygen species
Authors: Hsiao CH, Li W, Lou TF, Baliga BS, Pace BS.
Journal: Exp Hematol (2006): 264
Authors: Hsiao CH, Li W, Lou TF, Baliga BS, Pace BS.
Journal: Exp Hematol (2006): 264
DNA damage promotes histone deacetylase 4 nuclear localization and repression of G2/M promoters, via p53 C-terminal lysines
Authors: Basile V, Mantovani R, Imbriano C.
Journal: J Biol Chem (2006): 2347
Authors: Basile V, Mantovani R, Imbriano C.
Journal: J Biol Chem (2006): 2347
Real-time gene expression analysis in human xenografts for evaluation of histone deacetylase inhibitors
Authors: Belien A, De Schepper S, Floren W, Janssens B, Marien A, King P, Van Dun J, Andries L, Voeten J, Bijnens L, Janicot M, Arts J.
Journal: Mol Cancer Ther (2006): 2317
Authors: Belien A, De Schepper S, Floren W, Janssens B, Marien A, King P, Van Dun J, Andries L, Voeten J, Bijnens L, Janicot M, Arts J.
Journal: Mol Cancer Ther (2006): 2317
Arabidopsis thaliana histone deacetylase 1 (AtHD1) is localized in euchromatic regions and demonstrates histone deacetylase activity in vitro
Authors: Fong PM, Tian L, Chen ZJ.
Journal: Cell Res (2006): 479
Authors: Fong PM, Tian L, Chen ZJ.
Journal: Cell Res (2006): 479
Role of histone deacetylase Rpd3 in regulating rRNA gene transcription and nucleolar structure in yeast
Authors: Oakes ML, Siddiqi I, French SL, Vu L, Sato M, Aris JP, Beyer AL, Nomura M.
Journal: Mol Cell Biol (2006): 3889
Authors: Oakes ML, Siddiqi I, French SL, Vu L, Sato M, Aris JP, Beyer AL, Nomura M.
Journal: Mol Cell Biol (2006): 3889
Experimental therapy of malignant gliomas using the inhibitor of histone deacetylase MS-275
Authors: Eyupoglu IY, Hahnen E, Trankle C, Savaskan NE, Siebzehnrubl FA, Buslei R, Lemke D, Wick W, Fahlbusch R, Blumcke I.
Journal: Mol Cancer Ther (2006): 1248
Authors: Eyupoglu IY, Hahnen E, Trankle C, Savaskan NE, Siebzehnrubl FA, Buslei R, Lemke D, Wick W, Fahlbusch R, Blumcke I.
Journal: Mol Cancer Ther (2006): 1248
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