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MUGlcU [4-Methylumbelliferyl-beta-D-glucuronide] *CAS 881005-91-0*

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Physical properties
Molecular weight352.29
SolventDMSO
Spectral properties
Excitation (nm)341
Emission (nm)441
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


See also: Coumarins
CAS
881005-91-0
Molecular weight
352.29
Excitation (nm)
341
Emission (nm)
441
MUGlcU is a fluorogenic MU substrate for detecting glucuronidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. The organic and inorganic esters and glycosides of 7-hydroxy-4-methylcoumarin (4-methylumbelliferone, MU) are among the most commonly used fluorogenic substrates. These substrates yield water-soluble blue fluorescent coumarin fluorophores. MU develops maximum fluorescence above pH 10.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MUGlcU [4-Methylumbelliferyl-beta-D-glucuronide] *CAS 881005-91-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM283.857 µL1.419 mL2.839 mL14.193 mL28.386 mL
5 mM56.771 µL283.857 µL567.714 µL2.839 mL5.677 mL
10 mM28.386 µL141.929 µL283.857 µL1.419 mL2.839 mL

Molarity calculator

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Spectrum


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Spectral properties

Excitation (nm)341
Emission (nm)441

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References


View all 4 references: Citation Explorer
Coumarin Schiff-bases: as antioxidant and possibly anti-inflammatory agents
Authors: Gacche RN, Gond DS, Dhole NA, Dawane BS.
Journal: J Enzyme Inhib Med Chem (2006): 157
Examination of lectins, polysaccharopeptide, polysaccharide, alkaloid, coumarin and trypsin inhibitors for inhibitory activity against human immunodeficiency virus reverse transcriptase and glycohydrolases
Authors: Wang HX, Ng TB.
Journal: Planta Med (2001): 669
Induction of indoleamine 2,3-dioxygenase in alveolar interstitial cells of mouse lung by bacterial lipopolysaccharide
Authors: Urade Y, Yoshida R, Kitamura H, Hayaishi O.
Journal: J Biol Chem (1983): 6621
Determination of dicumarol metabolites in bile of rats
Authors: Conway WD, Lee FH, Neufeld L.
Journal: J Pharm Sci (1975): 1158