logo
AAT Bioquest

Resorufin beta-D-galactopyranoside *CAS 95079-19-9*

Product Image
Product Image
Gallery Image 1
Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight375.33
SolventDMSO
Spectral properties
Absorbance (nm)570
Extinction coefficient (cm -1 M -1)650001
Excitation (nm)571
Emission (nm)584
Quantum yield0.751
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


CAS
95079-19-9
Molecular weight
375.33
Absorbance (nm)
570
Extinction coefficient (cm -1 M -1)
650001
Excitation (nm)
571
Emission (nm)
584
Quantum yield
0.751
Resorufin beta-D-galactopyranoside is a fluorogenic galactosidase substrate that generates a red fluorescent product upon interaction with the enzyme. This enzyme substrate is widely used in high throughput screening of galactosidase inhibitors.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Resorufin beta-D-galactopyranoside *CAS 95079-19-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM266.432 µL1.332 mL2.664 mL13.322 mL26.643 mL
5 mM53.286 µL266.432 µL532.864 µL2.664 mL5.329 mL
10 mM26.643 µL133.216 µL266.432 µL1.332 mL2.664 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Absorbance (nm)570
Extinction coefficient (cm -1 M -1)650001
Excitation (nm)571
Emission (nm)584
Quantum yield0.751

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yield
Resorufin alpha-D-galactopyranoside5715846500010.751

Images


References


View all 8 references: Citation Explorer
Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited
Authors: English BP, Min W, van Oijen AM, Lee KT, Luo G, Sun H, Cherayil BJ, Kou SC, Xie XS.
Journal: Nat Chem Biol (2006): 87
Measurement of enzyme kinetics using a continuous-flow microfluidic system
Authors: Seong GH, Heo J, Crooks RM.
Journal: Anal Chem (2003): 3161
Crystallization of beta-galactosidase does not reduce the range of activity of individual molecules
Authors: Shoemaker GK, Juers DH, Coombs JM, Matthews BW, Craig DB.
Journal: Biochemistry (2003): 1707
beta-galactosidase assay using capillary electrophoresis laser-induced fluorescence detection and resorufin-beta-D-galactopyranoside as substrate
Authors: Eggertson MJ, Craig DB.
Journal: Biomed Chromatogr (1999): 516
Microchip device for performing enzyme assays
Authors: Hadd AG, Raymond DE, Halliwell JW, Jacobson SC, Ramsey JM.
Journal: Anal Chem (1997): 3407
Synthesis of a new substrate for detection of lacZ gene expression in live Drosophila embryos
Authors: Minden JS., undefined
Journal: Biotechniques (1996): 122
Expression of beta-galactosidase under the control of the human c-myc promoter in transgenic mice is inhibited by mithramycin
Authors: Jones DE, Jr., Cui DM, Miller DM.
Journal: Oncogene (1995): 2323
A sensitive competitive ELISA for 2,4-dinitrophenol using 3,6-fluorescein diphosphate as a fluorogenic substrate
Authors: Huang Z, Olson NA, You W, Haugl and RP., undefined
Journal: J Immunol Methods (1992): 261