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ROS Brite™ 570 *Optimized for Detecting Reactive Oxygen Species (ROS)*

Fluorescence response of ROS Brite™ 570 to different reactive oxygen species in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 540/590 nm.
Fluorescence response of ROS Brite™ 570 to different reactive oxygen species in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 540/590 nm.
Fluorescence response of ROS Brite™ 570 to different reactive oxygen species in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 540/590 nm.
The ferroptosis inhibitors (DFO, Ferro-1), the ROS scavengers (Trolox, Tempol), the apoptosis inhibitor (Z-VAD), the necroptosis inhibitor (Nec-1), or the autophagy inhibitor (CQ) cannot rescue TXNRD1 inhibitor-induced cell death in pre-OCs. ROS levels were detected by ROS Brite 570 using flow cytometry. Top row: BMDMs and pre-OCs was measured after 24 h of incubation with a medium containing 5 μM TRi-1 with or without 2 μM Ferrostantin-1 or 50 μM DFO. Bottom row: BMDMs and pre-OCs was measured after 5 h of incubation with a medium containing 2 μM AF or 5 μM TRi-1with or without 100 μM Trolox or 25 μM Tempol. n = 3 per group. All data in this figure are represented as mean ± SD. ns, no significance, *P < 0.01, **P < 0.01, ***P < 0.001, ****P < 0.0001. Source: <b>NFATc1-mediated expression of SLC7A11 drives sensitivity to TXNRD1 inhibitors in osteoclast precursors</b> by Zhong, et.al., <em>Redox Biology</em>, April 2023.
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Physical properties
Molecular weight732.81
SolventDMSO
Spectral properties
Excitation (nm)555
Emission (nm)568
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
732.81
Excitation (nm)
555
Emission (nm)
568
Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen. Examples include superoxide, hydroxyl radical, singlet oxygen and peroxides. ROS is highly reactive due to the presence of unpaired valence shell electrons. ROS forms as a natural byproduct of the normal metabolism of oxygen and have important roles in cell signaling and homeostasis. However, during times of environmental stress (e.g., UV or heat exposure), ROS levels can increase dramatically. This may result in significant damage to cell structures. Cumulatively, this is known as oxidative stress. ROS are also generated by exogenous sources such as ionizing radiation. Under conditions of oxidative stress, ROS production is dramatically increased, resulting in subsequent alteration of membrane lipids, proteins, and nucleic acids. Oxidative damage of these biomolecules is associated with aging as well as with a variety of pathological events, including atherosclerosis, carcinogenesis, ischemic reperfusion injury, and neurodegenerative disorders. ROS Brite™ 570 reagent is a new fluorogenic probe to measure oxidative stress in cells using conventional fluorescence microscopy, high-content imaging, microplate fluorometry, or flow cytometry. The cell-permeant ROS Brite™ 570 reagent is nonfluorescent and produces bright orange fluorescence upon ROS oxidation. The resulting fluorescence can be measured using fluorescence imaging, high-content imaging, microplate fluorometry, or flow cytometry. It is an excellent alternative to CellROX™ Orange Reagent (C10443) for oxidative stress detection (CellROX™ is a trademark of ThermoFisher).

Platform


Fluorescence microscope

ExcitationCy3/TRITC filter set
EmissionCy3/TRITC filter set
Recommended plateBlack wall/clear bottom
Instrument specification(s)Cy3/TRITC filter set

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ROS Brite™ 570 *Optimized for Detecting Reactive Oxygen Species (ROS)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM136.461 µL682.305 µL1.365 mL6.823 mL13.646 mL
5 mM27.292 µL136.461 µL272.922 µL1.365 mL2.729 mL
10 mM13.646 µL68.231 µL136.461 µL682.305 µL1.365 mL

Molarity calculator

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Spectrum


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Spectral properties

Excitation (nm)555
Emission (nm)568

Images


Citations


View all 10 citations: Citation Explorer
NFATc1-mediated expression of SLC7A11 drives sensitivity to TXNRD1 inhibitors in osteoclast precursors
Authors: Zhong, Zeyuan and Zhang, Chongjing and Ni, Shuo and Ma, Miao and Zhang, Xiaomeng and Sang, Weicong and Lv, Tao and Qian, Zhi and Yi, Chengqing and Yu, Baoqing
Journal: Redox Biology (2023): 102711
Low level of antioxidant capacity biomarkers but not target overexpression predicts vulnerability to ROS-inducing drugs
Authors: Samarin, Jana and Fabrowski, Piotr and Kurilov, Roman and Nuskova, Hana and Hummel-Eisenbeiss, Johanna and Pink, Hannelore and Li, Nan and Weru, Vivienn and Alborzinia, Hamed and Yildiz, Umut and others,
Journal: bioRxiv (2023): 2023--01
Involvement of a flavoprotein, acetohydroxyacid synthase, in growth and riboflavin production in riboflavin-overproducing Ashbya gossypii mutant
Authors: Kato, Tatsuya and Kano, Mai and Yokomori, Ami and Azegami, Junya and El Enshasy, Hesham A and Park, Enoch Y
Journal: Microbial Cell Factories (2023): 1--10
Toxin-Enabled “On-Demand” Liposomes for Enhanced Phototherapy to Treat and Protect against Methicillin-Resistant Staphylococcus aureus Infection
Authors: Zhuge, Deli and Chen, Mengchun and Yang, Xuewei and Zhang, Xufei and Yao, Lulu and Li, Li and Wang, Haonan and Chen, Hao and Yin, Qingqing and Tian, Dongyan and others,
Journal: Small (2022): 2203292
Mechanisms of Nanoparticle Toxicity in Cancer and Normal Cells
Authors: Tzelepi, Konstantina Nadia
Journal: (2019)
Thiol-Mediated Synthesis of Hyaluronic Acid-Epigallocatechin-3-O-Gallate Conjugates for the Formation of Injectable Hydrogels with Free Radical Scavenging Property and Degradation Resistance
Authors: Liu, Chixuan and Bae, Ki Hyun and Yamashita, Atsushi and Chung, Joo Eun and Kurisawa, Motoichi
Journal: Biomacromolecules (2017)
Thiol-mediated synthesis of hyaluronic acid--epigallocatechin-3-O-gallate conjugates for the formation of injectable hydrogels with free radical scavenging property and degradation resistance
Authors: Liu, Chixuan and Bae, Ki Hyun and Yamashita, Atsushi and Chung, Joo Eun and Kurisawa, Motoichi
Journal: Biomacromolecules (2017): 3143--3155
Transient receptor potential melastatin 8 ion channel in macrophages modulates colitis through a balance-shift in TNF-alpha and interleukin-10 production
Authors: Khalil, M and Babes, A and Lakra, R and F&ouml;rsch, S and Reeh, PW and Wirtz, S and Becker, C and Neurath, MF and Engel, MA
Journal: Mucosal immunology (2016)
VEGFR2 signaling prevents colorectal cancer cell senescence to promote tumorigenesis in mice with colitis
Authors: Foersch, Sebastian and Sperka, Tobias and Lindner, Christina and Taut, Astrid and Rudolph, Karl L and Breier, Georg and Boxberger, Frank and Rau, Tilman T and Hartmann, Arndt and St&uuml;rzl, Michael and others, undefined
Journal: Gastroenterology (2015): 177--189
Oxidative Stress--An Update and Insight in the Romanian Family Physician’s Adoption of the Concept
Authors: Berghea, Florian and Berghea, Camelia Elena and Abobului, Mihai
Journal: Internal Medicine : 11--15

References


View all 91 references: Citation Explorer
Lipoxin A inhibits porphyromonas gingivalis-induced aggregation and reactive oxygen species production by modulating neutrophil-platelet interaction and CD11b expression
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Journal: Infect Immun (2011): 1489
Role of hyaluronan and CD44 in reactive oxygen species-induced mucus hypersecretion
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Journal: Mol Cell Biochem (2011): 65
Nickel nanowires induced and reactive oxygen species mediated apoptosis in human pancreatic adenocarcinoma cells
Authors: Hossain MZ, Kleve MG.
Journal: Int J Nanomedicine (2011): 1475
Effect of glucocorticoid on production of reactive oxygen species in bone microvascular endothelial cells
Authors: Yang Y, Lou J, Li Z, Sun W, Wang B, Jia Y.
Journal: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2011): 533
Regulation of cyclooxygenase-2 and cytosolic phospholipase A2 gene expression by lipopolysaccharide through the RNA-binding protein HuR: involvement of NADPH oxidase, reactive oxygen species and mitogen-activated protein kinases
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Journal: Br J Pharmacol (2011): 1691
Effects of hypothermic storage on intracellular calcium, reactive oxygen species formation, mitochondrial function, motility, and plasma membrane integrity in striped bass (Morone saxatilis) sperm
Authors: Guthrie HD, Welch GR, Theisen DD, Woods LC, 3rd.
Journal: Theriogenology (2011): 951
Coenzyme Q functionalized CdTe/ZnS quantum dots for reactive oxygen species (ROS) imaging
Authors: Qin LX, Ma W, Li DW, Li Y, Chen X, Kraatz HB, James TD, Long YT.
Journal: Chemistry (2011): 5262
The role of reactive oxygen species in WP 631-induced death of human ovarian cancer cells: a comparison with the effect of doxorubicin
Authors: Rogalska A, Gajek A, Szwed M, Jozwiak Z, Marczak A.
Journal: Toxicol In Vitro (2011): 1712
Positive correlation between the generation of reactive oxygen species and activation/reactivation of transgene expression after hydrodynamic injections into mice
Authors: Takiguchi N, Takahashi Y, Nishikawa M, Matsui Y, Fukuhara Y, Oushiki D, Kiyose K, Hanaoka K, Nagano T, Takakura Y.
Journal: Pharm Res (2011): 702
Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells
Authors: Zhang YH, Wu YL, Tashiro S, Onodera S, Ikejima T.
Journal: Acta Pharmacol Sin (2011): 1266