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iFluor® 647 goat anti-mouse IgG (H+L)

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 647 goat anti-mouse IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~654 nm and ~674 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 647 goat anti-mouse IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 350 goat anti-mouse IgG (H+L)3454502000010.9510.830.23
iFluor® 405 goat anti-mouse IgG (H+L)4034273700010.9110.480.77
iFluor® 488 goat anti-mouse IgG (H+L)4915167500010.910.210.11
iFluor® 514 goat anti-mouse IgG (H+L)5115277500010.8310.2650.116
iFluor® 532 goat anti-mouse IgG (H+L)5375609000010.6810.260.16
iFluor® 555 goat anti-mouse IgG (H+L)55757010000010.6410.230.14
iFluor® 594 goat anti-mouse IgG (H+L)58760320000010.5310.050.04
iFluor® 633 goat anti-mouse IgG (H+L)64065425000010.2910.0620.044
iFluor® 680 goat anti-mouse IgG (H+L)68470122000010.2310.0970.094
iFluor® 700 goat anti-mouse IgG (H+L)69071322000010.2310.090.04
iFluor® 750 goat anti-mouse IgG (H+L)75777927500010.1210.0440.039
iFluor® 790 goat anti-mouse IgG (H+L)78781225000010.1310.10.09
iFluor® 350 goat anti-mouse IgG (H+L) *Cross Adsorbed*3454502000010.9510.830.23
iFluor® 405 goat anti-mouse IgG (H+L) *Cross Adsorbed*4034273700010.9110.480.77
iFluor® 488 goat anti-mouse IgG (H+L) *Cross Adsorbed*4915167500010.910.210.11
iFluor® 514 goat anti-mouse IgG (H+L) *Cross Adsorbed*5115277500010.8310.2650.116
iFluor® 532 goat anti-mouse IgG (H+L) *Cross Adsorbed*5375609000010.6810.260.16
iFluor® 555 goat anti-mouse IgG (H+L) *Cross Adsorbed*55757010000010.6410.230.14
iFluor® 594 goat anti-mouse IgG (H+L) *Cross Adsorbed*58760320000010.5310.050.04
iFluor® 633 goat anti-mouse IgG (H+L) *Cross Adsorbed*64065425000010.2910.0620.044
iFluor® 680 goat anti-mouse IgG (H+L) *Cross Adsorbed*68470122000010.2310.0970.094
iFluor® 700 goat anti-mouse IgG (H+L) *Cross Adsorbed*69071322000010.2310.090.04
iFluor® 750 goat anti-mouse IgG (H+L) *Cross Adsorbed*75777927500010.1210.0440.039
iFluor® 790 goat anti-mouse IgG (H+L) *Cross Adsorbed*78781225000010.1310.10.09
iFluor® 647 goat anti-rabbit IgG (H+L)65667025000010.2510.030.03
iFluor® 647 goat anti-rabbit IgG (H+L) *Cross Adsorbed*65667025000010.2510.030.03
iFluor® 546 goat anti-mouse IgG (H+L)54155710000010.6710.250.15
iFluor® 546 goat anti-mouse IgG (H+L) *Cross Adsorbed*54155710000010.6710.250.15
iFluor® 568 goat anti-mouse IgG (H+L)56858710000010.5710.340.15
iFluor® 568 goat anti-mouse IgG (H+L) *Cross Adsorbed*56858710000010.5710.340.15
iFluor® 800 goat anti-mouse IgG (H+L) 80182025000010.1110.030.08
iFluor® 800 goat anti-mouse IgG (H+L) *Cross Adsorbed*80182025000010.1110.030.08
iFluor® 810 goat anti-mouse IgG (H+L) 81182225000010.0510.090.15
iFluor® 810 goat anti-mouse IgG (H+L) *Cross Adsorbed*81182225000010.0510.090.15
iFluor® 820 goat anti-mouse IgG (H+L)82285025000010.110.16
iFluor® 820 goat anti-mouse IgG (H+L) *Cross Adsorbed*82285025000010.110.16
iFluor® 840 goat anti-mouse IgG (H+L)8368792000001-0.20.09
iFluor® 840 goat anti-mouse IgG (H+L) *Cross Adsorbed*8368792000001-0.20.09
iFluor® 860 goat anti-mouse IgG (H+L)85387825000010.10.14
iFluor® 860 goat anti-mouse IgG (H+L) *Cross Adsorbed*85387825000010.10.14
Show More (31)

Citations

View all 4 citations: Citation Explorer
Overexpression of CXCR2 predicts poor prognosis in patients with colorectal cancer.
Authors: Zhao, Jingkun and Ou, Baochi and Feng, Hao and Wang, Puxiongzhi and Yin, Shuai and Zhu, Congcong and Wang, Shenjie and Chen, Chun and Zheng, Minhua and Zong, Yaping and others, undefined
Journal: Oncotarget (2017)
Cadherin-12 enhances proliferation in colorectal cancer cells and increases progression by promoting EMT
Authors: Ma, Junjun and Zhao, Jingkun and Lu, Jun and Wang, Puxiongzhi and Feng, Hao and Zong, Yaping and Ou, Baochi and Zheng, Minhua and Lu, Aiguo
Journal: Tumor Biology (2016): 1--12
Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury
Authors: Shi, W and Huang, CJ and Xu, XD and Jin, GH and Huang, RQ and Huang, JF and Chen, YN and Ju, SQ and Wang, Y and Shi, YW and others, undefined
Journal: Acta Biomaterialia (2016): 247--261
The migration and differentiation of hUC-MSCsCXCR4/GFP encapsulated in BDNF/chitosan scaffolds for brain tissue engineering
Authors: Huang, Chuanjun and Zhao, Longxiang and Gu, Jun and Nie, Dekang and Chen, Yinan and Zuo, Hao and Huan, Wei and Shi, Jinlong and Chen, Jian and Shi, Wei
Journal: Biomedical Materials (2016): 035004

References

View all 191 references: Citation Explorer
Antiprothrombin antibodies in a patient with secondary antiphospholipid syndrome and bleeding
Authors: Gonzalez Leon R, Garcia Hern and ez FJ, Castillo Palma MJ, Sanchez Roman J.
Journal: Med Clin (Barc) (2011): 668
Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers
Authors: Waterhouse BR, Gijsen M, Barber PR, Tullis ID, Vojnovic B, Kong A.
Journal: Oncotarget (2011): 728
Falsely elevated tacrolimus levels caused by immunoassay interference secondary to beta-galactosidase antibodies in an infected liver transplant recipient
Authors: Knorr JP, Grewal KS, Balasubramanian M, Young N, Zaki R, Khanmoradi K, Araya V, Ortiz J.
Journal: Pharmacotherapy (2010): 954
Prevalence of anti-Epstein-Barr virus antibodies in children and adolescents with secondary immunodeficiency
Authors: Buckova A., undefined
Journal: Epidemiol Mikrobiol Imunol (2010): 133
Analysis of the effectiveness of reused primary and secondary antibodies in Western blotting analysis
Authors: Boonrod K, Roth B, Leong Ngar S, Krczal G.
Journal: Anal Biochem (2010): 124
Page updated on September 18, 2024

Ordering information

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Unit size
200 ug
1 mg
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Physical properties

Molecular weight

~150000

Solvent

Water

Spectral properties

Correction Factor (260 nm)

0.03

Correction Factor (280 nm)

0.03

Correction Factor (656 nm)

0.0793

Extinction coefficient (cm -1 M -1)

2500001

Excitation (nm)

656

Emission (nm)

670

Quantum yield

0.251

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
HeLa cells were incubated with mouse anti-tubulin followed by AAT&rsquo;s iFluor<sup>TM</sup> 647 goat anti-mouse IgG conjugate (Red, Left) or Alexa Fluor<sup>&reg;</sup> 647 goat anti-mouse IgG<sup>&nbsp; </sup>(Red, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).
HeLa cells were incubated with mouse anti-tubulin followed by AAT&rsquo;s iFluor<sup>TM</sup> 647 goat anti-mouse IgG conjugate (Red, Left) or Alexa Fluor<sup>&reg;</sup> 647 goat anti-mouse IgG<sup>&nbsp; </sup>(Red, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).
HeLa cells were incubated with mouse anti-tubulin followed by AAT&rsquo;s iFluor<sup>TM</sup> 647 goat anti-mouse IgG conjugate (Red, Left) or Alexa Fluor<sup>&reg;</sup> 647 goat anti-mouse IgG<sup>&nbsp; </sup>(Red, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).
<strong>Expression of IDO in antigen-treated DCs.</strong> At 7d, each BMDC treatment group in experiment (2) was collected. (A). the kynurenine content in the supernatant was measured by ELISA. The numbers in Y-axis indicate the range of concentrations of kynurenine in the supernatant. Bars represent means &plusmn; SEM. (B). The mRNA content of IDO in each treatment group was detected by real time qPCR. The experiment was performed in triplicate and the mean values were reported. Bars represent means &plusmn; SEM. (C).Double label fluorescence immunohistochemistry staining and confocal laser scanning microscope showed IDO expression in different group. Under fluorescence microscopy, IDO was stained with red, the cell nucleus was stained as green with CFSE. The white arrows in the figures represent the cells expression IDO. (D). The ratio of IDO+DC/IDO&ndash;DC in different groups: â–“ indicates number of cells not expressing IDO; â—¼indicates number of cells expressing IDO. Control means DCs are in RPMI1640 culture medium. # P&lt;0.01, significantly different from the control group. P&gt;&lt;0.01, significantly different from the group in the absence of 1-MT.&gt; Source:&nbsp;<strong>Mechanisms underlying immune tolerance caused by recombinant Echinococcus granulosus antigens Eg mMDH and Eg10 in dendritic cells</strong> by Yana Wang et al., <em>PLOS</em>, Sept. 2018.