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iFluor® 594 goat anti-mouse IgG (H+L)

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 594 goat anti-mouse IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~592 nm and ~614 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 594 goat anti-mouse IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 350 goat anti-mouse IgG (H+L)3454502000010.9510.830.23
iFluor® 405 goat anti-mouse IgG (H+L)4034273700010.9110.480.77
iFluor® 488 goat anti-mouse IgG (H+L)4915167500010.910.210.11
iFluor® 514 goat anti-mouse IgG (H+L)5115277500010.8310.2650.116
iFluor® 532 goat anti-mouse IgG (H+L)5375609000010.6810.260.16
iFluor® 555 goat anti-mouse IgG (H+L)55757010000010.6410.230.14
iFluor® 633 goat anti-mouse IgG (H+L)64065425000010.2910.0620.044
iFluor® 647 goat anti-mouse IgG (H+L)65667025000010.2510.030.03
iFluor® 680 goat anti-mouse IgG (H+L)68470122000010.2310.0970.094
iFluor® 700 goat anti-mouse IgG (H+L)69071322000010.2310.090.04
iFluor® 750 goat anti-mouse IgG (H+L)75777927500010.1210.0440.039
iFluor® 790 goat anti-mouse IgG (H+L)78781225000010.1310.10.09
iFluor® 350 goat anti-mouse IgG (H+L) *Cross Adsorbed*3454502000010.9510.830.23
iFluor® 405 goat anti-mouse IgG (H+L) *Cross Adsorbed*4034273700010.9110.480.77
iFluor® 488 goat anti-mouse IgG (H+L) *Cross Adsorbed*4915167500010.910.210.11
iFluor® 514 goat anti-mouse IgG (H+L) *Cross Adsorbed*5115277500010.8310.2650.116
iFluor® 532 goat anti-mouse IgG (H+L) *Cross Adsorbed*5375609000010.6810.260.16
iFluor® 555 goat anti-mouse IgG (H+L) *Cross Adsorbed*55757010000010.6410.230.14
iFluor® 633 goat anti-mouse IgG (H+L) *Cross Adsorbed*64065425000010.2910.0620.044
iFluor® 647 goat anti-mouse IgG (H+L) *Cross Adsorbed*65667025000010.2510.030.03
iFluor® 680 goat anti-mouse IgG (H+L) *Cross Adsorbed*68470122000010.2310.0970.094
iFluor® 700 goat anti-mouse IgG (H+L) *Cross Adsorbed*69071322000010.2310.090.04
iFluor® 750 goat anti-mouse IgG (H+L) *Cross Adsorbed*75777927500010.1210.0440.039
iFluor® 790 goat anti-mouse IgG (H+L) *Cross Adsorbed*78781225000010.1310.10.09
iFluor® 594 goat anti-rabbit IgG (H+L)58760320000010.5310.050.04
iFluor® 594 goat anti-rabbit IgG (H+L) *Cross Adsorbed*58760320000010.5310.050.04
iFluor® 546 goat anti-mouse IgG (H+L)54155710000010.6710.250.15
iFluor® 546 goat anti-mouse IgG (H+L) *Cross Adsorbed*54155710000010.6710.250.15
iFluor® 568 goat anti-mouse IgG (H+L)56858710000010.5710.340.15
iFluor® 568 goat anti-mouse IgG (H+L) *Cross Adsorbed*56858710000010.5710.340.15
iFluor® 800 goat anti-mouse IgG (H+L) 80182025000010.1110.030.08
iFluor® 800 goat anti-mouse IgG (H+L) *Cross Adsorbed*80182025000010.1110.030.08
iFluor® 810 goat anti-mouse IgG (H+L) 81182225000010.0510.090.15
iFluor® 810 goat anti-mouse IgG (H+L) *Cross Adsorbed*81182225000010.0510.090.15
iFluor® 820 goat anti-mouse IgG (H+L)82285025000010.110.16
iFluor® 820 goat anti-mouse IgG (H+L) *Cross Adsorbed*82285025000010.110.16
iFluor® 840 goat anti-mouse IgG (H+L)8368792000001-0.20.09
iFluor® 840 goat anti-mouse IgG (H+L) *Cross Adsorbed*8368792000001-0.20.09
iFluor® 860 goat anti-mouse IgG (H+L)85387825000010.10.14
iFluor® 860 goat anti-mouse IgG (H+L) *Cross Adsorbed*85387825000010.10.14
Show More (31)

Citations

View all 9 citations: Citation Explorer
Effects and mechanism of action of neonatal versus adult astrocytes on neural stem cell proliferation after traumatic brain injury
Authors: Dai, Yong and Sun, Feifan and Zhu, Hui and Liu, Qianqian and Xu, Xide and Gong, Peipei and Jiang, Rui and Jin, Guohua and Qin, Jianbing and Chen, Jian and others,
Journal: Stem Cells (2019): 1344--1356
Overexpression of CXCR2 predicts poor prognosis in patients with colorectal cancer
Authors: Zhao, Jingkun and Ou, Baochi and Feng, Hao and Wang, Puxiongzhi and Yin, Shuai and Zhu, Congcong and Wang, Shenjie and Chen, Chun and Zheng, Minhua and Zong, Yaping and others,
Journal: Oncotarget (2017): 28442
Up-regulation of chemokine receptor CCR4 is associated with Human Hepatocellular Carcinoma malignant behavior
Authors: Cheng, Xi and Wu, Huo and Jin, Zhi-Jian and Ma, Ding and Yuen, Stanley and Jing, Xiao-Qian and Shi, Min-Min and Shen, Bai-Yong and Peng, Cheng-Hong and Zhao, Ren and others,
Journal: Scientific reports (2017): 1--14
Overexpression of CXCR2 predicts poor prognosis in patients with colorectal cancer.
Authors: Zhao, Jingkun and Ou, Baochi and Feng, Hao and Wang, Puxiongzhi and Yin, Shuai and Zhu, Congcong and Wang, Shenjie and Chen, Chun and Zheng, Minhua and Zong, Yaping and others, undefined
Journal: Oncotarget (2017)

References

View all 191 references: Citation Explorer
Antiprothrombin antibodies in a patient with secondary antiphospholipid syndrome and bleeding
Authors: Gonzalez Leon R, Garcia Hern and ez FJ, Castillo Palma MJ, Sanchez Roman J.
Journal: Med Clin (Barc) (2011): 668
Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers
Authors: Waterhouse BR, Gijsen M, Barber PR, Tullis ID, Vojnovic B, Kong A.
Journal: Oncotarget (2011): 728
Falsely elevated tacrolimus levels caused by immunoassay interference secondary to beta-galactosidase antibodies in an infected liver transplant recipient
Authors: Knorr JP, Grewal KS, Balasubramanian M, Young N, Zaki R, Khanmoradi K, Araya V, Ortiz J.
Journal: Pharmacotherapy (2010): 954
Three siblings with self-resolving congenital hyperthyrotropinaemia secondary to thyrotropin receptor blocking antibodies
Authors: Azzopardi P, Forrester M, Ehtisham S.
Journal: J Paediatr Child Health (2010): 439
Nanosilver-doped DNA polyion complex membrane for electrochemical immunoassay of carcinoembryonic antigen using nanogold-labeled secondary antibodies
Authors: Wu W, Yi P, He P, Jing T, Liao K, Yang K, Wang H.
Journal: Anal Chim Acta (2010): 126
Page updated on September 18, 2024

Ordering information

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Unit size
200 ug
1 mg
Catalog Number
Quantity
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Physical properties

Molecular weight

~150000

Solvent

Water

Spectral properties

Absorbance (nm)

587

Correction Factor (260 nm)

0.05

Correction Factor (280 nm)

0.04

Extinction coefficient (cm -1 M -1)

2000001

Excitation (nm)

587

Emission (nm)

603

Quantum yield

0.531

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
HeLa cells were stained with mouse anti-tubulin followed with iFluor<sup>TM</sup> 594 goat anti-mouse IgG (H+L) (red); actin filaments were stained with Phalloidin-iFluor<sup>TM</sup> 488 conjugate (green); and nuclei were stained with DAPI (blue).
HeLa cells were stained with mouse anti-tubulin followed with iFluor<sup>TM</sup> 594 goat anti-mouse IgG (H+L) (red); actin filaments were stained with Phalloidin-iFluor<sup>TM</sup> 488 conjugate (green); and nuclei were stained with DAPI (blue).
HeLa cells were stained with mouse anti-tubulin followed with iFluor<sup>TM</sup> 594 goat anti-mouse IgG (H+L) (red); actin filaments were stained with Phalloidin-iFluor<sup>TM</sup> 488 conjugate (green); and nuclei were stained with DAPI (blue).
HeLa cells&nbsp;were stained&nbsp;with (Tubulin+) or without (Tubulin-) mouse anti-tubulin and then&nbsp;visualized&nbsp;with&nbsp;iFluor® 594 goat anti-mouse IgG (Left) or with Alexa Fluor&reg; 594 goat anti-mouse IgG (Right).