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Thiazole orange *10 mM in DMSO*

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Physical properties
Molecular weight476.61
SolventDMSO
Spectral properties
Excitation (nm)514
Emission (nm)533
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134

OverviewpdfSDSpdfProtocol


See also: Nucleus
CAS
107091-89-4
Molecular weight
476.61
Excitation (nm)
514
Emission (nm)
533
Thiazole orange is a fluorescent DNA-binding dye for reticulocyte analysis. It is lso useful for Plasmodium species analysis. The modified thiazole orange derivatives (such as SYBR Green, PicoGreen, TOTO and YOYO) are wdiely used for detecting DNA samples.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Thiazole orange *10 mM in DMSO* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM209.815 µL1.049 mL2.098 mL10.491 mL20.982 mL
5 mM41.963 µL209.815 µL419.63 µL2.098 mL4.196 mL
10 mM20.982 µL104.908 µL209.815 µL1.049 mL2.098 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Excitation (nm)514
Emission (nm)533

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
Acridine orange49052027000
Acridine orange *10 mg/mL solution in water*49052027000
CytoTell™ Orange541560-
LysoBrite™ Orange543565-

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References


View all 52 references: Citation Explorer
Large dynamic Stokes shift of DNA intercalation dye Thiazole Orange has contribution from a high-frequency mode
Authors: Karunakaran V, Perez Lustres JL, Zhao L, Ernsting NP, Seitz O.
Journal: J Am Chem Soc (2006): 2954
Effect of UVC-Induced Photooxidation of DNA on the Intercalation of Thiazole Orange: A Convenient Reporter for Oxidative Damage
Authors: Trevithick-Sutton CC, Mikelsons L, Filippenko V, Scaiano JC.
Journal: Photochem Photobiol. (2006)
Thiazole orange, a DNA-binding photosensitizer with flexible structure, can inactivate pathogens in red blood cell suspensions while maintaining red cell storage properties
Authors: Skripchenko A, Wagner SJ, Thompson-Montgomery D, Awatefe H.
Journal: Transfusion (2006): 213
Fluorescence resonance energy transfer and complex formation between thiazole orange and various dye-DNA conjugates: implications in signaling nucleic acid hybridization
Authors: Algar WR, Massey M, Krull UJ.
Journal: J Fluoresc (2006): 555
Exploring Base-Pair-Specific Optical Properties of the DNA Stain Thiazole Orange
Authors: Jarikote DV, Krebs N, Tannert S, Roder B, Seitz O.
Journal: Chemistry. (2006)
Sequence-specific fluorescence detection of DNA by polyamide-thiazole orange conjugates
Authors: Fechter EJ, Olenyuk B, Dervan PB.
Journal: J Am Chem Soc (2005): 16685
Synthesis and fluorescence studies of thiazole orange tethered onto oligonucleotide: development of a self-contained DNA biosensor on a fiber optic surface
Authors: Wang X, Krull UJ.
Journal: Bioorg Med Chem Lett (2005): 1725
Forced intercalation probes (FIT Probes): thiazole orange as a fluorescent base in peptide nucleic acids for homogeneous single-nucleotide-polymorphism detection
Authors: Kohler O, Jarikote DV, Seitz O.
Journal: Chembiochem (2005): 69
Thiazole orange positive platelets in a dog with Evans' syndrome
Authors: Michimoto T, Okamura T, Suzuki K, Watari T, Kano R, Hasegawa A.
Journal: J Vet Med Sci (2004): 1305
Thiazole orange-peptide conjugates: sensitivity of DNA binding to chemical structure
Authors: Carreon JR, Mahon KP, Jr., Kelley SO.
Journal: Org Lett (2004): 517