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Nuclear Orange™ DCS1 *5 mM DMSO Solution*

Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with iFluor 350 Phalloidin (Cat#23110, Blue) and nuclei stain Nuclear Orange™ DCS1 (Cat#17551, Red)
Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with iFluor 350 Phalloidin (Cat#23110, Blue) and nuclei stain Nuclear Orange™ DCS1 (Cat#17551, Red)
Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with iFluor 350 Phalloidin (Cat#23110, Blue) and nuclei stain Nuclear Orange™ DCS1 (Cat#17551, Red)
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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties
Molecular weightN/A
SolventDMSO
Spectral properties
Excitation (nm)514
Emission (nm)556
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134

OverviewpdfSDSpdfProtocol


Molecular weight
N/A
Excitation (nm)
514
Emission (nm)
556
Our Nuclear Orange™ DCS1 is a fluorogenic, DNA-selective and cell-impermeant dye for analyzing DNA content in dead, fixed or apoptotic cells. The Nuclear Orange™ DCS1 has its orange fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of dead, fixed or apoptotic cells with proper filter sets.

Platform


Fluorescence microscope

ExcitationCy3/TRITC Filter
EmissionCy3/TRITC Filter
Recommended plateBlack wall/clear bottom

Example protocol


AT A GLANCE

Spectral Properties

Ex/Em = 514/556 nm (bound to DNA) 

SAMPLE EXPERIMENTAL PROTOCOL

Caution: The following protocol can be adapted for most cell types. Growth medium, cell density, the presence of other cell types, and factors may influence staining. Residual detergent on glassware may also affect the staining of many organisms and cause brightly stained material to appear in solutions with or without cells present.

  1. Add Nuclear Orange™ DCS1 (2 to10 µM) into the fixed, dead or apoptotic cells (either suspension or adherent) and incubate the cells for 15 to 60 minutes.

    Note: In initial experiments, it is advisable to test a wide range of dye concentrations in order to determine the optimal concentration that yields the desired result.

    Optional: Wash the cells twice with Hanks and 20 mM HEPES buffer (HBSS) or a buffer of your choice. Then fill the wells with fresh HBSS or growth medium.

  2. Observe the cells using a fluorescence microscope, fluorescence microplate reader, or flow cytometer equipped with the desired filter set. 

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Excitation (nm)514
Emission (nm)556

Images


Citations


View all 2 citations: Citation Explorer
One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays
Authors: Dai, Sheng and Li, Rong and Long, Yan and Titus, Steve and Zhao, Jinghua and Huang, Ruili and Xia, Menghang and Zheng, Wei
Journal: Journal of Biomolecular Screening (2016): 1112--1124
Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production
Authors: Zhang, Guoqiang and Lin, Yuping and Qi, Xianni and Wang, Lixian and He, Peng and Wang, Qinhong and Ma, Yanhe
Journal: Microbial cell factories (2015): 1

References


View all 65 references: Citation Explorer
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Journal: J Antimicrob Chemother (2009): 751
Detection of methylation in hepatocellular carcinoma using SYBR Green fluorescent quantitative PCR
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Journal: Zhonghua Yi Xue Yi Chuan Xue Za Zhi (2008): 534
Estimation of copy number using SYBR Green: confounding by AT-rich DNA and by variation in amplicon length
Authors: Colborn JM, Byrd BD, Koita OA, Krogstad DJ.
Journal: Am J Trop Med Hyg (2008): 887
DRAQ5-based, no-lyse, no-wash bone marrow aspirate evaluation by flow cytometry
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Journal: Am J Clin Pathol (2008): 706
Interaction of a DNA intercalator DRAQ5, and a minor groove binder SYTO17, with chromatin in live cells--influence on chromatin organization and histone-DNA interactions
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Journal: Cytometry A (2008): 555
Addition of gold nanoparticles to real-time PCR: effect on PCR profile and SYBR Green I fluorescence
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Journal: Anal Bioanal Chem (2008): 887
Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I
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Journal: Nucleic Acids Res (2008): e93
Highly sensitive and selective detection of Hg(2+) in aqueous solution with mercury-specific DNA and Sybr Green I
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Group testing to annihilate pairs applied to DNA cross-hybridization elimination using SYBR Green I
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DRAQ5: improved flow cytometric DNA content analysis and minimal residual disease detection in childhood malignancies
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