LDS 751 CAS 181885-68-7

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Physical properties

Molecular weight	471.98
Solvent	DMSO

Spectral properties

Excitation (nm)	561
Emission (nm)	712

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Overview SDS Protocol

CAS

181885-68-7

Molecular weight

471.98

Excitation (nm)

561

Emission (nm)

712

LDS 751 is a fluorescent cell-permeant nucleic acid stain that can be well excited with 488 nm laser line although it has a peak excitation at ~543 nm on dsDNA. It might be an alternative to DRAQ 5™, useful in multicolor analyses due to its long wavelength emission maximum (~712 nm). Upon binding to dsDNA of LDS 751 has ~20-fold fluorescence enhancement. It has been reported that LDS-751 is excluded from the nucleus and binds the polarized membranes of mitochondria. Thus cautions need be taken when LDS 751 is used for analyzing live cells and LDS-751 fluorescence as being indicative of nuclear status. Thiazole orange (an RNA dye) and LDS-751 (a DNA dye) composition has been used to separate reticulocytes from other cell populations. LDS 751 has been also used to separate red blood cells from nucleated cells.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of LDS 751 *CAS 181885-68-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	211.873 µL	1.059 mL	2.119 mL	10.594 mL	21.187 mL
5 mM	42.375 µL	211.873 µL	423.747 µL	2.119 mL	4.237 mL
10 mM	21.187 µL	105.937 µL	211.873 µL	1.059 mL	2.119 mL

Molarity calculator

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Spectrum

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Spectral properties

Excitation (nm)	561
Emission (nm)	712

Product Family

Name	Excitation (nm)	Emission (nm)
LDS 651	557	630

Images

Figure 1. Chemical structure for LDS 751 *CAS 181885-68-7*

References

View all 29 references: Citation Explorer

Interaction of LDS-751 with the drug-binding site of P-glycoprotein: a Trp fluorescence steady-state and lifetime study
Authors: Lugo MR, Sharom FJ.
Journal: Arch Biochem Biophys (2009): 17

Hybridization-sensitive fluorescent probe for long-term monitoring of intracellular RNA
Authors: Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A.
Journal: Bioconjug Chem (2009): 1256

Exciton-controlled fluorescence: application to hybridization-sensitive fluorescent DNA probe
Authors: Okamoto A, Ikeda S, Kubota T, Yuki M, Yanagisawa H.
Journal: Nucleic Acids Symp Ser (Oxf) (2009): 49

Flow cytometric assessment of homeostatic aging of reticulocytes in rats
Authors: Wiczling P, Krzyzanski W.
Journal: Exp Hematol (2008): 119

Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry
Authors: Maes ML, Davidson LB, McDonagh PF, Ritter LS.
Journal: J Immunol Methods (2007): 79

Determination of the formation of dark state via depleted spontaneous emission in a complex solvated molecule
Authors: Guo X, Wang S, Xia A, Su H.
Journal: J Phys Chem A (2007): 5800

Interaction of LDS-751 and rhodamine 123 with P-glycoprotein: evidence for simultaneous binding of both drugs
Authors: Lugo MR, Sharom FJ.
Journal: Biochemistry (2005): 14020

Exciton mobility and trapping in a MALDI matrix
Authors: Setz PD, Knochenmuss R.
Journal: J Phys Chem A (2005): 4030

Cell cycle synchronization of Cupriavidus necator by continuous phasing measured via flow cytometry
Authors: Fritsch M, Starruss J, Loesche A, Mueller S, Bley T.
Journal: Biotechnol Bioeng (2005): 635

Quantitative evaluation of isothiocyanates as substrates and inhibitors of P-glycoprotein
Authors: Barecki-Roach M, Wang EJ, Johnson WW.
Journal: J Pharm Pharmacol (2003): 1251