Helixyte™ Gold Nucleic Acid Gel Stain 10,000X DMSO Solution

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	749.48
Solvent	DMSO

Spectral properties

Excitation (nm)	496
Emission (nm)	539

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Overview SDS Protocol

Molecular weight

749.48

Excitation (nm)

496

Emission (nm)

539

Helixyte™ Gold is manufactured by AAT Bioquest, and it has the same chemical structure of SYBR® Gold (SYBR® is the trademark of ThermoFisher). Helixyte™ Gold is an excellent nucleic acid gel stain, and exhibits large fluorescence enhancement upon binding to nucleic acids. It has the same spectral properties to those of SYBR® Gold, thus a great replacement to SYBR® Gold (SYBR® Gold is the trademark of ThermoFisher). It is one of the most sensitive stains available for detecting DNA in agarose and polyacrylamide gels. Helixyte™ Gold has higher sensitivity for DNA than RNA, and is ideal for use with laser scanners with the same instrument settings of SYBR Gold. Helixyte™ Gold is much more sensitive than ethidium bromide for DNA in agarose gels, and it can detect as low as picogram dsDNA on gels.

Example protocol

AT A GLANCE

Spectral Properties of Helixyte™ Gold
Excitation/Emission: 495/540 nm
Important Helixyte™ Gold nucleic acid gel stain is significantly less mutagenic than ethidium bromide. However, we must caution that no data are available on the mutagenicity or toxicity of Helixyte™ Gold stain in humans. It should be treated as a potential mutagen and used with appropriate care due to the fact that this reagent binds to nucleic acids. The disposing of the stain shall be in compliance with local regulations.
We have found the greatest sensitivity is achieved by post-staining which also eliminates the possibility of dye interference with DNA migration. While the precast protocol is more convenient, some DNA samples may experience migration, it is highly recommended the gel running time does not exceed more than 2 hours. The following protocols are recommended. However, some comparisons might be needed to determine which one better meets your needs.

PREPARATION OF WORKING SOLUTION

Helixyte™ Gold working solution (1X)

Make 1X Helixyte™ Gold working solution by diluting the 10,000X stock reagent into pH 7.5 - 8 buffer (e.g., TAE, TBE or TE, preferably pH 8.0).
Note Staining solutions prepared in water are less stable than those prepared in buffer and must be used within 24 hours to ensure maximal staining sensitivity.
Note In addition, staining solutions prepared in buffers with pH below 7.5 or above 8.0 are less stable and show reduced staining efficacy.

SAMPLE EXPERIMENTAL PROTOCOL

Post-Staining Protocol

Run gels based on your standard protocol.
Place the gel in a suitable polypropylene container. Gently add a sufficient amount of the 1X Helixyte™ Gold working solution to submerge the gel.
Note Do not use a glass container, as it will adsorb much of the dye in the staining solution.
Agitate the gel gently at room temperature for ~30 minutes, protected from the light.
Note The staining solution can be stored in the dark (preferably refrigerated) for a week and reused up to 2 - 3 times.
Image the stained gel with a 254 nm transilluminator or a laser-based gel scanner using a long path green filter, such as a SYBR® filter or GelStar® filter.

Pre-Casting Protocol

Prepare agarose gel solution using your standard protocol.
Add 1X Helixyte™ Gold working solution to the gel and mix thoroughly.
Run gels based on your standard protocol.
Image the stained gel with a 254 nm transilluminator or a laser-based gel scanner using a long path green filter, such as a SYBR® filter or GelStar® filter.

DNA-Staining Before Electrophoresis

Incubate DNA with a 1:1000 to 1:3000 dilution of the dye (in TE, TBE, or TAE) for at least 15 minutes prior to electrophoresis.
Run gels based on your standard protocol.
Image the stained gel with a 254 nm transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR® filter or GelStar® filter.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Helixyte™ Gold Nucleic Acid Gel Stain *10,000X DMSO Solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	133.426 µL	667.129 µL	1.334 mL	6.671 mL	13.343 mL
5 mM	26.685 µL	133.426 µL	266.852 µL	1.334 mL	2.669 mL
10 mM	13.343 µL	66.713 µL	133.426 µL	667.129 µL	1.334 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	496
Emission (nm)	539

Product Family

Name	Excitation (nm)	Emission (nm)
Helixyte™ Green Nucleic Acid Gel Stain 10,000X DMSO Solution	498	522

Images

Figure 1. 160 ng of 1 Kb Plus DNA Ladder (ThermoFisher 10787018) in 0.9% agarose/TBE electrophoresis gel were stained with Helixyte™ Gold and SYBR® Gold and imaged with 254-nm UV transilluminator using UVP Bioimaging System.

References

View all 79 references: Citation Explorer

SYBR green real-time PCR for the detection of all enterovirus-A71 genogroups
Authors: Dubot-Peres A, Tan CY, de Chesse R, Sibounheuang B, Vongsouvath M, Phommasone K, Bessaud M, Gazin C, Thirion L, Phetsouvanh R, Newton PN, de Lamballerie X.
Journal: PLoS One (2014): e89963

Development of a SYBR Green I based one-step real-time PCR assay for the detection of Hantaan virus
Authors: Jiang W, Wang PZ, Yu HT, Zhang Y, Zhao K, Du H, Bai XF.
Journal: J Virol Methods (2014): 145

A Broadly Reactive One-Step SYBR Green I Real-Time RT-PCR Assay for Rapid Detection of Murine Norovirus
Authors: Hanaki K, Ike F, Kajita A, Yasuno W, Yanagiba M, Goto M, Sakai K, Ami Y, Kyuwa S.
Journal: PLoS One (2014): e98108

A SYBR Green I based real time RT-PCR assay for specific detection and quantitation of Peste des petits ruminants virus
Authors: Abera T, Thangavelu A, Joy Ch and ran ND, Raja A.
Journal: BMC Vet Res (2014): 22

A SYBR-green I quantitative real-time reverse transcription-PCR assay for rabies viruses with different virulence
Authors: Wang L, Liu Y, Zhang S, Wang Y, Zhao J, Miao F, Hu R.
Journal: Virol Sin (2014): 131

Novel strategy combining SYBR Green I with carbon nanotubes for highly sensitive detection of Salmonella typhimurium DNA
Authors: Mao P, Ning Y, Li W, Peng Z, Chen Y, Deng L.
Journal: Enzyme Microb Technol (2014): 15

Detection and characterization of Leishmania (Leishmania) and Leishmania (Viannia) by SYBR green-based real-time PCR and high resolution melt analysis targeting kinetoplast minicircle DNA
Authors: Ceccarelli M, Galluzzi L, Migliazzo A, Magnani M.
Journal: PLoS One (2014): e88845

Molecular detection of human rhinoviruses in respiratory samples: a comparison of Taqman probe-, SYBR green I- and BOXTO-based real-time PCR assays
Authors: Dupouey J, Ninove L, Ferrier V, Py O, Gazin C, Thirion-Perrier L, de Lamballerie X.
Journal: Virol J (2014): 31

Development of SYBR Green based real time PCR assay for detection of monodon baculovirus in Penaeus monodon
Authors: Ramesh Kumar D, Sanjuktha M, Rajan JJ, An and a Bharathi R, Santiago TC, Alav and i SV, Poornima M.
Journal: J Virol Methods (2014): 81

Development of a SYBR Green real-time RT-PCR assay for the detection of avian encephalomyelitis virus
Authors: Liu Q, Yang Z, Hao H, Cheng S, Fan W, Du E, Xiao S, Wang X, Zhang S.
Journal: J Virol Methods (2014): 46