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Fluorescein, disodium salt *CAS 518-47-8*

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Physical properties
Molecular weight376.27
SolventWater
Spectral properties
Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


See also: Fluoresceins
CAS
518-47-8
Molecular weight
376.27
Absorbance (nm)
487
Correction Factor (260 nm)
0.32
Correction Factor (280 nm)
0.35
Extinction coefficient (cm -1 M -1)
800001
Excitation (nm)
498
Emission (nm)
517
Quantum yield
0.79001, 0.952
Fluorescein, disodium salt is water-soluble version of fluorescein (see product #1). Fluorescein is a fluorophore widely used in microscopy, forensics and serology to detect latent blood stains. Fluorescein has an absorption maximum at 490 nm and emission maximum of 514 nm (in water). It has a pKa at 6.4 and multiple ionization equilibria. This leads to pH dependent absorption and emission over the range of 5 to 9. It has an isoabsorptive point at 460 nm.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Fluorescein, disodium salt *CAS 518-47-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM265.767 µL1.329 mL2.658 mL13.288 mL26.577 mL
5 mM53.153 µL265.767 µL531.533 µL2.658 mL5.315 mL
10 mM26.577 µL132.883 µL265.767 µL1.329 mL2.658 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2*360448180000.1

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Citations


View all 2 citations: Citation Explorer
Fluorescence Guided Surgery
Authors: Stewart, Hazel L and Birch, David JS
Journal: Methods and Applications in Fluorescence (2021)

References


View all 91 references: Citation Explorer
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Journal: Br J Pharmacol (2011): 1691
Effects of hypothermic storage on intracellular calcium, reactive oxygen species formation, mitochondrial function, motility, and plasma membrane integrity in striped bass (Morone saxatilis) sperm
Authors: Guthrie HD, Welch GR, Theisen DD, Woods LC, 3rd.
Journal: Theriogenology (2011): 951
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Authors: Qin LX, Ma W, Li DW, Li Y, Chen X, Kraatz HB, James TD, Long YT.
Journal: Chemistry (2011): 5262
The role of reactive oxygen species in WP 631-induced death of human ovarian cancer cells: a comparison with the effect of doxorubicin
Authors: Rogalska A, Gajek A, Szwed M, Jozwiak Z, Marczak A.
Journal: Toxicol In Vitro (2011): 1712
Positive correlation between the generation of reactive oxygen species and activation/reactivation of transgene expression after hydrodynamic injections into mice
Authors: Takiguchi N, Takahashi Y, Nishikawa M, Matsui Y, Fukuhara Y, Oushiki D, Kiyose K, Hanaoka K, Nagano T, Takakura Y.
Journal: Pharm Res (2011): 702
Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells
Authors: Zhang YH, Wu YL, Tashiro S, Onodera S, Ikejima T.
Journal: Acta Pharmacol Sin (2011): 1266