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FluoroQuest™ Mounting Medium with DAPI

HeLa cells were stained with mouse anti-tubulin and then followed with iFluor<sup>TM</sup>&nbsp;610 goat anti-mouse IgG (H+L). Cells were&nbsp;mounted in FluoroQuest&trade; Mounting Medium with DAPI (Cat# 20004).
HeLa cells were stained with mouse anti-tubulin and then followed with iFluor<sup>TM</sup>&nbsp;610 goat anti-mouse IgG (H+L). Cells were&nbsp;mounted in FluoroQuest&trade; Mounting Medium with DAPI (Cat# 20004).
HeLa cells were stained with mouse anti-tubulin and then followed with iFluor<sup>TM</sup>&nbsp;610 goat anti-mouse IgG (H+L). Cells were&nbsp;mounted in FluoroQuest&trade; Mounting Medium with DAPI (Cat# 20004).
Gallery Image 2
Western corn rootworm midgut morphology after feeding with Vpb4Da2. Vpb4Da2 staining by Alexa-fluor-488 conjugated secondary antibody is in green. Nuclei staining by DAPI is in blue. (A) Longitudinal section of the midgut after 6 h exposure to Vpb4Da2. (B) Cross section after 48 h exposure to Vpb4Da2. (C) Cross section after 96 h exposure to Vpb4Da2. (A) and (B) White arrows indicate blebbing of the epithelial cells. (A), (B) and (C) White arrowheads designate a complete or near complete loss of the apical microvilli layer. (D), (E), and (F) Are untreated controls where black arrowheads indicate the intact apical microvilli layer. Scale bars are all 50 μm.
Source: <b>Structural and functional insights into the first Bacillus thuringiensis vegetative insecticidal protein of the Vpb4 fold, active against western corn rootworm</b> by
Kouadio JL, Zheng M, Aikins M, Duda D, Duff S, et al. <em>PLOS ONE</em> Dec. 2021.
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Physical properties
SolventWater
Spectral properties
Extinction coefficient (cm -1 M -1)27000
Excitation (nm)359
Emission (nm)457
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Extinction coefficient (cm -1 M -1)
27000
Excitation (nm)
359
Emission (nm)
457
FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This mounting medium is fortified with DAPI which is a counterstain for DNA. For use in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing a blue fluorescence. RNA is also stained with DAPI.

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Spectral properties

Extinction coefficient (cm -1 M -1)27000
Excitation (nm)359
Emission (nm)457

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Citations


View all 9 citations: Citation Explorer
Zika Virus-Infected Monocyte Exosomes Mediate Cell-to-Cell Viral Transmission
Authors: Mart{\'\i}nez-Rojas, Pedro Pablo and Monroy-Mart{\'\i}nez, Ver{\'o}nica and Agredano-Moreno, Lourdes Teresa and Jim{\'e}nez-Garc{\'\i}a, Luis Felipe and Ruiz-Ordaz, Blanca H
Journal: Cells (2024): 144
Structural and functional characterization of Mpp75Aa1. 1, a putative beta-pore forming protein from Brevibacillus laterosporus active against the western corn rootworm
Authors: Kouadio, Jean-Louis and Duff, Stephen and Aikins, Michael and Zheng, Meiying and Rydel, Timothy and Chen, Danqi and Bretsnyder, Eric and Xia, Chunsheng and Zhang, Jun and Milligan, Jason and others,
Journal: PloS one (2021): e0258052
Acute melatonin administration improves exercise tolerance and the metabolic recovery after exhaustive effort
Authors: Faria, Vin{\'\i}cius Silva and Pejon, Taciane Maria Melges and Gobatto, Claudio Alexandre and de Araujo, Gustavo Gomes and Cornachione, Anabelle Silva and Beck, Wladimir Rafael
Journal: Scientific Reports (2021): 1--11
Simultaneous hyperbaric oxygen therapy during systemic chemotherapy reverses chemotherapy-induced peripheral neuropathy by inhibiting TLR4 and TRPV1 activation in the central and peripheral nervous system
Authors: Chou, Ping-Ruey and Lu, Ching-Yeh and Kan, Jung-Yu and Wang, Shih-Hung and Lo, Jing-Jou and Huang, Shu-Hung and Wu, Sheng-Hua
Journal: Supportive Care in Cancer (2021): 1--10
Structural and functional insights into the first Bacillus thuringiensis vegetative insecticidal protein of the Vpb4 fold, active against western corn rootworm
Authors: Kouadio, Jean-Louis and Zheng, Meiying and Aikins, Michael and Duda, David and Duff, Stephen and Chen, Danqi and Zhang, Jun and Milligan, Jason and Taylor, Christina and Mamanella, Patricia and others,
Journal: PloS one (2021): e0260532
Comparison between the therapeutic effects of differentiated and undifferentiated Wharton's jelly mesenchymal stem cells in rats with streptozotocin-induced diabetes
Authors: Hsiao, Chen-Yuan and Chen, Tien-Hua and Huang, Ben-Shian and Chen, Po-Han and Su, Cheng-Hsi and Shyu, Jia-Fwu and Tsai, Pei-Jiun
Journal: World Journal of Stem Cells (2020): 139
Participation of extracellular vesicles from zika-virus-infected mosquito cells in the modification of na{\"\i}ve cells’ behavior by mediating cell-to-cell transmission of viral elements
Authors: Mart{\'\i}nez-Rojas, Pedro Pablo and Quiroz-Garc{\'\i}a, Elizabeth and Monroy-Mart{\'\i}nez, Ver{\'o}nica and Agredano-Moreno, Lourdes Teresa and Jim{\'e}nez-Garc{\'\i}a, Luis Felipe and Ruiz-Ordaz, Blanca H
Journal: Cells (2020): 123
Dose-Dependent Effect of Hyperbaric Oxygen Treatment on Burn-Induced Neuropathic Pain in Rats
Authors: Wu, Zong-Sheng and Wu, Sheng-Hua and Lee, Su-Shin and Lin, Cen-Hung and Chang, Chih-Hau and Lo, Jing-Jou and Chai, Chee-Yin and Wu, Ching-Shuang and Huang, Shu-Hung
Journal: International journal of molecular sciences (2019): 1951
Dose-dependent effect of hyperbaric oxygen treatment on burn-induced neuropathic pain in rats
Authors: Wu, Zong-Sheng and Wu, Sheng-Hua and Lee, Su-Shin and Lin, Cen-Hung and Chang, Chih-Hau and Lo, Jing-Jou and Chai, Chee-Yin and Wu, Ching-Shuang and Huang, Shu-Hung
Journal: International journal of molecular sciences (2019): 1951

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