DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] CAS 6268-49-1

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Physical properties

Molecular weight	269.3
Solvent	DMSO

Spectral properties

Absorbance (nm)	454
Correction Factor (280 nm)	0.516

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

6268-49-1

Molecular weight

269.3

Absorbance (nm)

454

Correction Factor (280 nm)

0.516

DABCYL acid is one of the most popular acceptors for developing FRET-based nucleic acid probes suh as Molecular Beacon-based PCR probes. It is also used to prepare a variety of FRET peptides that are often used for the detection of various proteases. It is predominantly paired with EDANS (as fluorescence donor) in FRET-based fluorescent probes.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	371.333 µL	1.857 mL	3.713 mL	18.567 mL	37.133 mL
5 mM	74.267 µL	371.333 µL	742.666 µL	3.713 mL	7.427 mL
10 mM	37.133 µL	185.667 µL	371.333 µL	1.857 mL	3.713 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Absorbance (nm)	454
Correction Factor (280 nm)	0.516

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (280 nm)	Correction Factor (260 nm)
ICG acid	789	813	230000	-	0.076	-
Cy3NS acid CAS 1032678-01-5	555	569	150000¹	0.15¹	0.073	0.07
Cy5NS acid	651	670	250000¹	0.27¹, 0.4²	0.03	0.02
Cy7NS acid	756	779	250000	0.3	0.036	0.05
AMCA acid CAS#: 106562-32-7	346	434	19000	-	0.153	-
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] CAS 50402-56-7	336	455	5900	-	0.107	-
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]	336	455	5900	-	0.107	-
BCECF acid CAS#: 85138-49-4	504	527	90000	-	-	-
XFD532 acid Same Structure to Alexa Fluor™ 532 acid	534	553	81000	0.61¹	0.09	0.24
XFD488 acid Same Structure to Alexa Fluor™ 488 acid	499	520	71000	0.92¹	0.11	0.30
Cy3B acid	560	571	120000¹	0.58¹	0.069	0.048
XFD350 acid Same Structure to Alexa Fluor™ 350 acid	343	441	19000	-	0.19	0.25
XFD546 acid Same Structure to Alexa Fluor™ 546 acid	561	572	112000	0.79¹	0.12	0.21
XFD568 acid Same Structure to Alexa Fluor™ 568 acid	579	603	91300	0.69¹	0.46	0.45
XFD514 acid	518	543	80000	-	0.18	0.31
QXY7 acid [equivalent to QSY-7 acid]	-	-	90000¹	-	0.22	-
XFD555 acid	553	568	155000	0.1¹	0.08	0.08
XFD647 acid	650	671	270000	0.33¹	0.03	-
XFD750 acid	752	776	290000	0.12¹	0.04	-
XFD700 acid	696	719	205000	0.25¹	0.07	-
OG514 acid [equivalent to Oregon Green® 514 carboxylic acid]	513	533	85000	-	0.19	-
Show More (12)

Images

Figure 1. Chemical structure for DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1*

Citations

View all 3 citations: Citation Explorer

Peptide Based Inhibitors of Protein binding to the Mitogen-Activated Protein Kinase Docking Groove
Authors: Alexa, Anita and Ember, Orsolya and Szab{\'o}, Ildik{\'o} and Mo'ath, Yousef and P{\'o}ti, {\'A}d{\'a}m L and Rem{\'e}nyi, Attila and B{\'a}n{\'o}czi, Zolt{\'a}n
Journal: Frontiers in molecular biosciences (2021): 629

Influence of the Dabcyl group on the cellular uptake of cationic peptides: short oligoarginines as efficient cell-penetrating peptides
Authors: Szab{\'o}, Ildik{\'o} and Illien, Fran{\c{c}}oise and D{\'o}kus, Levente E and Baranyai, Zsuzsa and B{\H{o}}sze, Szilvia and Ise, Shoko and Kawano, Kenichi and Sagan, Sandrine and Futaki, Shiroh and Hudecz, Ferenc and others,
Journal: Amino Acids (2021): 1--17

Protein N-terminal processing: substrate specificity of Escherichia coli and human methionine aminopeptidases
Authors: Xiao, Qing and Zhang, Feiran and Nacev, Benjamin A and Liu, Jun O and Pei, Dehua
Journal: Biochemistry (2010): 5588--5599

References

View all 34 references: Citation Explorer

Recombinant expression and partial characterization of an active soluble histo-aspartic protease from Plasmodium falciparum
Authors: Xiao H, Sinkovits AF, Bryksa BC, Ogawa M, Yada RY.
Journal: Protein Expr Purif (2006): 88

Internally quenched peptides for the study of lysostaphin: An antimicrobial protease that kills Staphylococcus aureus
Authors: Warfield R, Bardelang P, Saunders H, Chan WC, Penfold C, James R, Thomas NR.
Journal: Org Biomol Chem (2006): 3626

A quenched fluorescent dipeptide for assaying dispase- and thermolysin-like proteases
Authors: Weimer S, Oertel K, Fuchsbauer HL.
Journal: Anal Biochem (2006): 110

Characterization and inhibition of SARS-coronavirus main protease
Authors: Liang PH., undefined
Journal: Curr Top Med Chem (2006): 361

Fluorogenic peptide substrates containing benzoxazol-5-yl-alanine derivatives for kinetic assay of cysteine proteases
Authors: Szabelski M, Rogiewicz M, Wiczk W.
Journal: Anal Biochem (2005): 20

ADAM33 enzyme properties and substrate specificity
Authors: Zou J, Zhang R, Zhu F, Liu J, Madison V, Uml and SP., undefined
Journal: Biochemistry (2005): 4247

Enzymatic activity characterization of SARS coronavirus 3C-like protease by fluorescence resonance energy transfer technique
Authors: Chen S, Chen LL, Luo HB, Sun T, Chen J, Ye F, Cai JH, Shen JK, Shen X, Jiang HL.
Journal: Acta Pharmacol Sin (2005): 99

Fluorogenic peptide substrates for carboxydipeptidase activity of cathepsin B
Authors: Stachowiak K, Tokmina M, Karpinska A, Sosnowska R, Wiczk W.
Journal: Acta Biochim Pol (2004): 81

Synthesis of poly(ethylene glycol)-based saquinavir prodrug conjugates and assessment of release and anti-HIV-1 bioactivity using a novel protease inhibition assay
Authors: Gunaseelan S, Debrah O, Wan L, Leibowitz MJ, Rabson AB, Stein S, Sinko PJ.
Journal: Bioconjug Chem (2004): 1322

Highly sensitive intramolecularly quenched fluorogenic substrates for renin based on the combination of L-2-amino-3-(7-methoxy-4-coumaryl)propionic acid with 2,4-dinitrophenyl groups at various positions
Authors: Paschalidou K, Neumann U, Gerhartz B, Tzougraki C.
Journal: Biochem J (2004): 1031