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HHBS [Hanks' Buffer with 20 mM Hepes]
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Ordering information
| Price | |
| Catalog Number | |
| Availability | In stock |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Refrigerated (2-8 °C); Minimize light exposure |
| UNSPSC | 12352200 |
| Overview |  SDSProtocol |
See also: Buffers and Lab Consumables
Hank's Balanced Salt Solution with 20 mM Hepes buffer (HHBS) is used for a variety of cell culture applications, such as washing cells, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media, or for reagent preparation such as calcium, membrane potential assay buffer preparation.
Images
![HHBS [Hanks’ Buffer with 20 mM Hepes]](https://images.aatbio.com/products/figures-and-data/hhbs-hanks-buffer-with-20-mm-hepes/figure-for-hhbs-hanks-buffer-with-20-mm-hepes_UJNN2.jpg)
Figure 1. HHBS [Hanks’ Buffer with 20 mM Hepes]
Citations
View all 13 citations: Citation Explorer
Effects of extremely low frequency electromagnetic fields on the tumor cell inhibition and the possible mechanism
Authors: Sun, Jie and Tong, Yingying and Jia, Yu and Jia, Xu and Wang, Hua and Chen, Yang and Wu, Jiamin and Jin, Weiyang and Ma, Zheng and Cao, Kai and others,
Journal: Scientific Reports (2023): 6989
Authors: Sun, Jie and Tong, Yingying and Jia, Yu and Jia, Xu and Wang, Hua and Chen, Yang and Wu, Jiamin and Jin, Weiyang and Ma, Zheng and Cao, Kai and others,
Journal: Scientific Reports (2023): 6989
Complex intestinal and hepatic in vitro barrier models reveal information on uptake and impact of micro-, submicro-and nanoplastics
Authors: Paul, Maxi B and B{\"o}hmert, Linda and Hsiao, I-Lun and Braeuning, Albert and Sieg, Holger
Journal: Environment International (2023): 108172
Authors: Paul, Maxi B and B{\"o}hmert, Linda and Hsiao, I-Lun and Braeuning, Albert and Sieg, Holger
Journal: Environment International (2023): 108172
Characterization of a fluorescence imaging probe that exploits metabolic dependency of ovarian clear cell carcinoma
Authors: Tsuchimochi, Saki and Wada-Hiraike, Osamu and Urano, Yasuteru and Kukita, Asako and Yamaguchi, Kohei and Honjo, Harunori and Taguchi, Ayumi and Tanikawa, Michihiro and Sone, Kenbun and Mori-Uchino, Mayuyo and others,
Journal: Scientific Reports (2023): 20292
Authors: Tsuchimochi, Saki and Wada-Hiraike, Osamu and Urano, Yasuteru and Kukita, Asako and Yamaguchi, Kohei and Honjo, Harunori and Taguchi, Ayumi and Tanikawa, Michihiro and Sone, Kenbun and Mori-Uchino, Mayuyo and others,
Journal: Scientific Reports (2023): 20292
Identification of Autophagy as a Functional Target Suitable for the Pharmacological Treatment of Mitochondrial Membrane Protein-Associated Neurodegeneration (MPAN) In Vitro. Pharmaceutics 2023, 15, 267
Authors: Zanuttigh, E and Derderian, K and G{\"u}ra, MA and Geerlof, A and Di Meo, I and Cavestro, C and Hempfling, S and Ortiz-Collazos, S and Mauthe, M and Kmiec, T and others,
Journal: (2023)
Authors: Zanuttigh, E and Derderian, K and G{\"u}ra, MA and Geerlof, A and Di Meo, I and Cavestro, C and Hempfling, S and Ortiz-Collazos, S and Mauthe, M and Kmiec, T and others,
Journal: (2023)
Geraniol-Mediated Suppression of Endoplasmic Reticulum Stress Protects against Cerebral Ischemia--Reperfusion Injury via the PERK-ATF4-CHOP Pathway
Authors: Wu, Yu and Fan, Xiaomei and Chen, Sha and Deng, Ling and Jiang, Lu and Yang, Shaonan and Dong, Zhi
Journal: International Journal of Molecular Sciences (2022): 544
Authors: Wu, Yu and Fan, Xiaomei and Chen, Sha and Deng, Ling and Jiang, Lu and Yang, Shaonan and Dong, Zhi
Journal: International Journal of Molecular Sciences (2022): 544
Triple-Decker Sandwich Cultures of Intestinal Organoids for Long-Term Live Imaging, Uniform Perturbation, and Statistical Sampling
Authors: Cambra, Hailey M and Tallapragada, Naren P and Mannam, Prabhath and Breault, David T and Klein, Allon M
Journal: Current protocols (2022): e330
Authors: Cambra, Hailey M and Tallapragada, Naren P and Mannam, Prabhath and Breault, David T and Klein, Allon M
Journal: Current protocols (2022): e330
Identification and Functional Assays of Single Nucleotide Variants of Opsins Genes in Melanocytic Tumors
Authors: Zhang, Wei and Zeng, Wen and Feng, Jianglong and Li, Pinhao and Wang, Yu and Lu, Hongguang
Journal: Pigment Cell \& Melanoma Research (2022)
Authors: Zhang, Wei and Zeng, Wen and Feng, Jianglong and Li, Pinhao and Wang, Yu and Lu, Hongguang
Journal: Pigment Cell \& Melanoma Research (2022)
Myocyte-specific enhancer factor 2c triggers transdifferentiation of adipose tissue-derived stromal cells into spontaneously beating cardiomyocyte-like cells
Authors: Takashima, Shinichiro and Usui, Soichiro and Inoue, Oto and Goten, Chiaki and Yamaguchi, Kosei and Takeda, Yusuke and Cui, Shihe and Sakai, Yoshio and Hayashi, Kenshi and Sakata, Kenji and others,
Journal: Scientific reports (2021): 1--16
Authors: Takashima, Shinichiro and Usui, Soichiro and Inoue, Oto and Goten, Chiaki and Yamaguchi, Kosei and Takeda, Yusuke and Cui, Shihe and Sakai, Yoshio and Hayashi, Kenshi and Sakata, Kenji and others,
Journal: Scientific reports (2021): 1--16
Engineered Extracellular Matrices with Integrated Wireless Microactuators to Study Mechanobiology
Authors: Uslu, Fazil E and Davidson, Christopher D and Mailand, Erik and Bouklas, Nikolaos and Baker, Brendon M and Sakar, Mahmut Selman
Journal: Advanced Materials (2021): 2102641
Authors: Uslu, Fazil E and Davidson, Christopher D and Mailand, Erik and Bouklas, Nikolaos and Baker, Brendon M and Sakar, Mahmut Selman
Journal: Advanced Materials (2021): 2102641
Mypcyte-specific enhancer factor 2c triggers transdifferentiation of adipose tissue-derived stromal cells into spontaneously beating cardiomyocyte-like cells
Authors: Takashima, Shinichiro and Usui, Soichiro and Inoue, Oto and Goten, Chiaki and Yamaguchi, Kosei and Takeda, Yusuke and Cui, Shihe and Sakai, Yoshio and Hayashi, Kenshi and Sakata, Kenji and others,
Journal: Scientific reports (2021): 1-16
Authors: Takashima, Shinichiro and Usui, Soichiro and Inoue, Oto and Goten, Chiaki and Yamaguchi, Kosei and Takeda, Yusuke and Cui, Shihe and Sakai, Yoshio and Hayashi, Kenshi and Sakata, Kenji and others,
Journal: Scientific reports (2021): 1-16
Application notes
FAQ
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How to lyse cells?
How can I lyse my cells without lysing the nuclear membrane?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
Why is my GSH concentration greater than my Total GSH concentration? Why is my calculated GSH/TGSH ratio greater than 1?