Probenecid Cell culture tested CAS 57-66-9

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Physical properties

Molecular weight	285.36
Solvent	1 M NaOH

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Room temperature (10-25 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

CAS

57-66-9

Molecular weight

285.36

Probenecid is an inhibitor of organic-anion transporters located in cell membranes. These transporters often extrude fluorescent indicators from cells, and therefore contribute to poor dye retention. This phenomenon usually causes high background in the assays that require the good retention of the dye indicators inside cells. The use of probenecid to inhibit the transporter activity, and thus to reduce leakage of the intracellular dye indicators is a common method for reducing fluorescence background of calcium assays. Each vial is dissovled in 0.3 mL of 1 M NaOH, and then diluted into 100 mL buffer to make 2.5 mM final concentration of probenecid for your assays.

Calculators

Common stock solution preparation

Table 1. Volume of 1 M NaOH needed to reconstitute specific mass of Probenecid *Cell culture tested* *CAS 57-66-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	350.435 µL	1.752 mL	3.504 mL	17.522 mL	35.043 mL
5 mM	70.087 µL	350.435 µL	700.869 µL	3.504 mL	7.009 mL
10 mM	35.043 µL	175.217 µL	350.435 µL	1.752 mL	3.504 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Images

Figure 1. Chemical structure for Probenecid *Cell culture tested* *CAS 57-66-9*

Citations

View all 12 citations: Citation Explorer

Identification of the material basis of the medicinal properties in Curcuma Longa L. to enhance targeted clinical application
Authors: Peng, Sha and Li, Jing and Huo, Mengqi and Cao, Yanfeng and Chen, Zhao and Zhang, Yanling and Qiao, Yanjiang
Journal: Journal of Traditional Chinese Medical Sciences (2022): 374--382

Triple-Decker Sandwich Cultures of Intestinal Organoids for Long-Term Live Imaging, Uniform Perturbation, and Statistical Sampling
Authors: Cambra, Hailey M and Tallapragada, Naren P and Mannam, Prabhath and Breault, David T and Klein, Allon M
Journal: Current protocols (2022): e330

Magnetic-field-synchronized wireless modulation of neural activity by magnetoelectric nanoparticles
Authors: Zhang, E and Abdel-Mottaleb, M and Liang, P and Navarrete, B and Yildirim, Y Akin and Campos, M Alberteris and Smith, IT and Wang, P and Yildirim, B and Yang, L and others,
Journal: Brain Stimulation (2022): 1451--1462

Pharmacogenomic screening of drug candidates using patient-specific hiPSC-derived cardiomyocyte high-throughput calcium imaging
Authors: Blancard, Malorie and Fetterman, K Ashley and Burridge, Paul W
Journal: (2022): 241--253

A novel Ca2+ indicator for long-term tracking of intracellular calcium flux
Authors: Liao, Jinfang and Patel, Deven and Zhao, Qin and Peng, Ruogu and Guo, Haitao and Diwu, Zhenjun
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Electrophysiological Effects of Extracellular Vesicles Secreted by Cardiosphere-Derived Cells: Unraveling the Antiarrhythmic Properties of Cell Therapies
Authors: G{\'o}mez-Cid, Lidia and Moro-L{\'o}pez, Marina and de la Nava, Ana S and Hern{\'a}ndez-Romero, Ismael and Fern{\'a}ndez, Ana I and Su{\'a}rez-Sancho, Susana and Atienza, Felipe and Grigorian-Shamagian, Lilian and Fern{\'a}ndez-Avil{\'e}s, Francisco
Journal: Processes (2020): 924

Integrative Cardiovascular Physiology and Pathophysiology: Absence of gravin-mediated signaling inhibits development of high-fat diet-induced hyperlipidemia and atherosclerosis
Authors: Fan, Qiying and Yin, Xing and Rababa’h, Abeer and Diaz, Andrea Diaz and Wijaya, Cori S and Singh, Sonal and Suryavanshi, Santosh V and Vo, Henry Hiep and Saeed, Moawiz and Zhang, Yang and others,
Journal: American Journal of Physiology-Heart and Circulatory Physiology (2019): H793

Etablierung eines 3D in vitro Blutgef{\"a}{\ss}-/Gewebemodells zur Testung spezifischer Therapeutika zur Leuk{\"a}miebehandlung
Authors: Bersi, Heidi
Journal: (2017)

Teleocidin A2 inhibits human proteinase-activated receptor 2 signaling in tumor cells
Authors: Stahn, Sonja and Thelen, Lisa and Albrecht, Ina-Maria and Bitzer, Jens and Henkel, Thomas and Teusch, Nicole Elisabeth
Journal: Pharmacology Research & Perspectives (2016)

Gravin Scaffolding Protein Mediates Signaling during Atherosclerosis
Authors: Fan, Qiying
Journal: (2015)

References

View all 35 references: Citation Explorer

DNA damage caused by benzo(a)pyrene in MCF-7 cells is increased by verapamil, probenecid and PSC833
Authors: Myllynen P, Kurttila T, Vaskivuo L, Vahakangas K.
Journal: Toxicol Lett (2007): 3

Is the monkey an appropriate animal model to examine drug-drug interactions involving renal clearance? Effect of probenecid on the renal elimination of H2 receptor antagonists
Authors: Tahara H, Kusuhara H, Chida M, Fuse E, Sugiyama Y.
Journal: J Pharmacol Exp Ther (2006): 1187

Inhibition of oat3-mediated renal uptake as a mechanism for drug-drug interaction between fexofenadine and probenecid
Authors: Tahara H, Kusuhara H, Maeda K, Koepsell H, Fuse E, Sugiyama Y.
Journal: Drug Metab Dispos (2006): 743

MRP2 (ABCC2) transports taxanes and confers paclitaxel resistance and both processes are stimulated by probenecid
Authors: Huisman MT, Chhatta AA, van Tellingen O, Beijnen JH, Schinkel AH.
Journal: Int J Cancer (2005): 824

Mediation of annexin 1 secretion by a probenecid-sensitive ABC-transporter in rat inflamed mucosa
Authors: Wein S, Fauroux M, Laffitte J, de Nadai P, Guaini C, Pons F, Comera C.
Journal: Biochem Pharmacol (2004): 1195

Open randomized study of pyrimethamine-sulphadoxine vs. pyrimethamine-sulphadoxine plus probenecid for the treatment of uncomplicated Plasmodium falciparum malaria in children
Authors: Sowunmi A, Fehintola FA, Adedeji AA, Gbotosho GO, Falade CO, Tambo E, Fateye BA, Happi TC, Oduola AM.
Journal: Trop Med Int Health (2004): 606

Inhibition of multidrug transporters by verapamil or probenecid does not alter blood-brain barrier penetration of levetiracetam in rats
Authors: Potschka H, Baltes S, Loscher W.
Journal: Epilepsy Res (2004): 85

Probenecid interacts with the pharmacokinetics of morphine-6-glucuronide in humans
Authors: Skarke C, Langer M, Jarrar M, Schmidt H, Geisslinger G, Lotsch J.
Journal: Anesthesiology (2004): 1394

Impact of Mrp2 on the biliary excretion and intestinal absorption of furosemide, probenecid, and methotrexate using Eisai hyperbilirubinemic rats
Authors: Chen C, Scott D, Hanson E, Franco J, Berryman E, Volberg M, Liu X.
Journal: Pharm Res (2003): 31

The influence of inhibition of probenecid sensitive transporters on the central nervous system (CNS) uptake and the antinociceptive activity of morphine-6-glucuronide in rats
Authors: Lotsch J, Schmidt R, Vetter G, Schmidt H, Skarke C, Niederberger E, Geisslinger G, Tegeder I.
Journal: Neurosci Lett (2002): 145