ReadiUse™ probenecid 25 mM stabilized aqueous solution

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	307.34
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Molecular weight

307.34

Probenecid is an inhibitor of organic-anion transporters located in cell membranes. These transporters often extrude fluorescent indicators from cells, and therefore contribute to poor dye retention. This phenomenon usually causes high background in the assays that require the good retention of the dye indicators inside cells. The use of probenecid to inhibit the transporter activity, and thus to reduce leakage of the intracellular dye indicators is a common method for reducing fluorescence background of calcium assays. The commonly used free acid form of probenecid requires the use of 1 M NaOH to dissolve it due to its poor water solubility in neutral water. AAT Bioquest offer the convenient Readiuse™ water-soluble and heat-stable probenecid in the format of a powder, solution or tablet. They are convenient to use and are as effective as the free acid form at the same concentration. Each vial is diluted with 90 mL buffer to make 2.5 mM final concentration of probenecid for your assays.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of ReadiUse™ probenecid *25 mM stabilized aqueous solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	325.373 µL	1.627 mL	3.254 mL	16.269 mL	32.537 mL
5 mM	65.075 µL	325.373 µL	650.745 µL	3.254 mL	6.507 mL
10 mM	32.537 µL	162.686 µL	325.373 µL	1.627 mL	3.254 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Product Family

Name	Excitation (nm)	Emission (nm)
ReadiUse™ CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] CAS 150347-59-4	498	517
ReadiUse™ PE [R-Phycoerythrin] Ammonium Sulfate-Free	565	574

Images

Figure 1. Chemical structure for ReadiUse™ probenecid *25 mM stabilized aqueous solution*

Citations

View all 9 citations: Citation Explorer

Identification of the material basis of the medicinal properties in Curcuma Longa L. to enhance targeted clinical application
Authors: Peng, Sha and Li, Jing and Huo, Mengqi and Cao, Yanfeng and Chen, Zhao and Zhang, Yanling and Qiao, Yanjiang
Journal: Journal of Traditional Chinese Medical Sciences (2022): 374--382

Magnetic-field-synchronized wireless modulation of neural activity by magnetoelectric nanoparticles
Authors: Zhang, E and Abdel-Mottaleb, M and Liang, P and Navarrete, B and Yildirim, Y Akin and Campos, M Alberteris and Smith, IT and Wang, P and Yildirim, B and Yang, L and others,
Journal: Brain Stimulation (2022): 1451--1462

Pharmacogenomic screening of drug candidates using patient-specific hiPSC-derived cardiomyocyte high-throughput calcium imaging
Authors: Blancard, Malorie and Fetterman, K Ashley and Burridge, Paul W
Journal: (2022): 241--253

Hypocalcemia in sepsis: analysis of the subcellular distribution of Ca2+ in septic rats and LPS/TNF-$\alpha$-treated HUVECs
Authors: He, Wencheng and Huang, Lei and Luo, Hua and Zang, Yang and An, Youzhong and Zhang, Weixing
Journal: The Journal of Infection in Developing Countries (2020): 908--917

Absence of Gravin Mediated Signaling Inhibits Development of High Fat Diet-Induced Hyperlipidemia and Atherosclerosis
Authors: Fan, Qiying and Yin, Xing and Rababa'h, Abeer and Diaz Diaz, Andrea and Wijaya, Cori S and Singh, Sonal and Suryavanshi, Santosh V and Vo, Henry Hiep and Saeed, Moawiz and Zhang, Yang and others, undefined
Journal: American Journal of Physiology-Heart and Circulatory Physiology (2019)

Teleocidin A2 inhibits human proteinase-activated receptor 2 signaling in tumor cells
Authors: Stahn, Sonja and Thelen, Lisa and Albrecht, Ina-Maria and Bitzer, Jens and Henkel, Thomas and Teusch, Nicole Elisabeth
Journal: Pharmacology Research & Perspectives (2016)

Gravin Scaffolding Protein Mediates Signaling during Atherosclerosis
Authors: Fan, Qiying
Journal: (2015)

Glutathione adducts on sarcoplasmic/endoplasmic reticulum Ca2+ ATPase Cys-674 regulate endothelial cell calcium stores and angiogenic function as well as promote ischemic blood flow recovery
Authors: Thompson, Melissa D and Mei, Yu and Weisbrod, Robert M and Silver, Marcy and Shukla, Praphulla C and Bolotina, Victoria M and Cohen, Richard A and Tong, Xiaoyong
Journal: Journal of Biological Chemistry (2014): 19907--19916

Redox regulation of cysteine-674 of SERCA 2 is critical for growth factor-and ischemia-induced angiogenesis
Authors: Thompson, Melissa Danielle
Journal: (2014)

References

View all 35 references: Citation Explorer

DNA damage caused by benzo(a)pyrene in MCF-7 cells is increased by verapamil, probenecid and PSC833
Authors: Myllynen P, Kurttila T, Vaskivuo L, Vahakangas K.
Journal: Toxicol Lett (2007): 3

Is the monkey an appropriate animal model to examine drug-drug interactions involving renal clearance? Effect of probenecid on the renal elimination of H2 receptor antagonists
Authors: Tahara H, Kusuhara H, Chida M, Fuse E, Sugiyama Y.
Journal: J Pharmacol Exp Ther (2006): 1187

Inhibition of oat3-mediated renal uptake as a mechanism for drug-drug interaction between fexofenadine and probenecid
Authors: Tahara H, Kusuhara H, Maeda K, Koepsell H, Fuse E, Sugiyama Y.
Journal: Drug Metab Dispos (2006): 743

MRP2 (ABCC2) transports taxanes and confers paclitaxel resistance and both processes are stimulated by probenecid
Authors: Huisman MT, Chhatta AA, van Tellingen O, Beijnen JH, Schinkel AH.
Journal: Int J Cancer (2005): 824

Mediation of annexin 1 secretion by a probenecid-sensitive ABC-transporter in rat inflamed mucosa
Authors: Wein S, Fauroux M, Laffitte J, de Nadai P, Guaini C, Pons F, Comera C.
Journal: Biochem Pharmacol (2004): 1195

Open randomized study of pyrimethamine-sulphadoxine vs. pyrimethamine-sulphadoxine plus probenecid for the treatment of uncomplicated Plasmodium falciparum malaria in children
Authors: Sowunmi A, Fehintola FA, Adedeji AA, Gbotosho GO, Falade CO, Tambo E, Fateye BA, Happi TC, Oduola AM.
Journal: Trop Med Int Health (2004): 606

Inhibition of multidrug transporters by verapamil or probenecid does not alter blood-brain barrier penetration of levetiracetam in rats
Authors: Potschka H, Baltes S, Loscher W.
Journal: Epilepsy Res (2004): 85

Probenecid interacts with the pharmacokinetics of morphine-6-glucuronide in humans
Authors: Skarke C, Langer M, Jarrar M, Schmidt H, Geisslinger G, Lotsch J.
Journal: Anesthesiology (2004): 1394

Impact of Mrp2 on the biliary excretion and intestinal absorption of furosemide, probenecid, and methotrexate using Eisai hyperbilirubinemic rats
Authors: Chen C, Scott D, Hanson E, Franco J, Berryman E, Volberg M, Liu X.
Journal: Pharm Res (2003): 31

The influence of inhibition of probenecid sensitive transporters on the central nervous system (CNS) uptake and the antinociceptive activity of morphine-6-glucuronide in rats
Authors: Lotsch J, Schmidt R, Vetter G, Schmidt H, Skarke C, Niederberger E, Geisslinger G, Tegeder I.
Journal: Neurosci Lett (2002): 145