Cal-590™, potassium salt
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Dissociation constant (Kd, nM) | 561 |
Molecular weight | 1123.03 |
Solvent | Water |
Spectral properties
Extinction coefficient (cm -1 M -1) | 78000 |
Excitation (nm) | 574 |
Emission (nm) | 588 |
Quantum yield | 0.621 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Related products
Overview | SDSProtocol |
See also: Calcium Indicators, Multi-Photon Microscopy
Molecular weight 1123.03 | Dissociation constant (Kd, nM) 561 | Extinction coefficient (cm -1 M -1) 78000 | Excitation (nm) 574 | Emission (nm) 588 | Quantum yield 0.621 |
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-2 is most commonly used among the red fluorescent calcium indicators. However, Rhod-2 AM is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses. Cal-590™ has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2, making it compatible with TRITC/Cy3® filter set. In CHO and HEK cells Cal-590™ AM has cellular calcium response that is much more sensitive than Rhod-2 AM. The spectra of Cal-590 is well separated from those of FITC, Alexa Fluor® 488 and GFP, making it an ideal calcium probe for multiplexing intracellular assays with GFP cell lines or FITC/Alexa Fluor® 488 labeled antibodies.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Cal-590™, potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 89.045 µL | 445.224 µL | 890.448 µL | 4.452 mL | 8.904 mL |
5 mM | 17.809 µL | 89.045 µL | 178.09 µL | 890.448 µL | 1.781 mL |
10 mM | 8.904 µL | 44.522 µL | 89.045 µL | 445.224 µL | 890.448 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
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Spectral properties
Extinction coefficient (cm -1 M -1) | 78000 |
Excitation (nm) | 574 |
Emission (nm) | 588 |
Quantum yield | 0.621 |
Product Family
Name | Excitation (nm) | Emission (nm) | Quantum yield |
Cal-590™, sodium salt | 574 | 588 | 0.621 |
Cal-630™, potassium salt | 609 | 626 | 0.371 |
Cal-520®, potassium salt | 492 | 515 | 0.751 |
Cal-520FF™, potassium salt | 492 | 515 | 0.751 |
Cal-520N™, potassium salt | 492 | 515 | 0.751 |
Calbryte™ 590, potassium salt | 581 | 593 | - |
Cal-500™, potassium salt | 388 | 482 | 0.481 |
Cal-670™, potassium salt | 667 | 680 | - |
Cal-770™, potassium salt | 758 | 783 | - |
Images
Citations
View all 8 citations: Citation Explorer
Gap junction coupling shapes the encoding of light in the developing retina
Authors: Caval-Holme, Franklin and Zhang, Yizhen and Feller, Marla B
Journal: Current Biology (2019): 4024--4035
Authors: Caval-Holme, Franklin and Zhang, Yizhen and Feller, Marla B
Journal: Current Biology (2019): 4024--4035
A versatile and Open-Source rapid LED switching system for One-Photon imaging and Photo-Activation
Authors: Battefeld, Arne and Popovic, Marko A and Van der Werf, Dirk and Kole, Maarten HP
Journal: Frontiers in cellular neuroscience (2019): 530
Authors: Battefeld, Arne and Popovic, Marko A and Van der Werf, Dirk and Kole, Maarten HP
Journal: Frontiers in cellular neuroscience (2019): 530
Development of a deep two-photon calcium imaging method for the analysis of cortical processing in the mammalian brain
Authors: Birkner, Antje
Journal: (2019)
Authors: Birkner, Antje
Journal: (2019)
A versatile and open-source rapid LED switching system for one-photon imaging and photo-activation
Authors: Battefeld, Arne and Popovic, Marko A and Van der Werf, Dirk and Kole, Maarten HP
Journal: Frontiers in cellular neuroscience (2018)
Authors: Battefeld, Arne and Popovic, Marko A and Van der Werf, Dirk and Kole, Maarten HP
Journal: Frontiers in cellular neuroscience (2018)
Ca 2+ signals initiate at immobile IP 3 receptors adjacent to ER-plasma membrane junctions
Authors: Thillaiappan, Nagendra Babu and Chavda, Alap P and Tovey, Stephen C and Prole, David L and Taylor, Colin W
Journal: Nature Communications (2017): 1505
Authors: Thillaiappan, Nagendra Babu and Chavda, Alap P and Tovey, Stephen C and Prole, David L and Taylor, Colin W
Journal: Nature Communications (2017): 1505
α V β 3 Integrin regulates astrocyte reactivity
Authors: Lagos-Cabré, Raúl and Alvarez, Alvaro and Kong, Milene and Burgos-Bravo, Francesca and Cárdenas, Areli and Rojas-Mancilla, Edgardo and Pérez-Nunez, Ramón and Herrera-Molina, Rodrigo and Rojas, Fabiola and Schneider, Pascal and others, undefined
Journal: Journal of Neuroinflammation (2017): 194
Authors: Lagos-Cabré, Raúl and Alvarez, Alvaro and Kong, Milene and Burgos-Bravo, Francesca and Cárdenas, Areli and Rojas-Mancilla, Edgardo and Pérez-Nunez, Ramón and Herrera-Molina, Rodrigo and Rojas, Fabiola and Schneider, Pascal and others, undefined
Journal: Journal of Neuroinflammation (2017): 194
In vivo deep two-photon imaging of neural circuits with the fluorescent Ca2+ indicator Cal-590
Authors: Tischbirek, Carsten H and Birkner, Antje and Konnerth, Arthur
Journal: The Journal of Physiology (2016)
Authors: Tischbirek, Carsten H and Birkner, Antje and Konnerth, Arthur
Journal: The Journal of Physiology (2016)
Deep two-photon brain imaging with a red-shifted fluorometric Ca2+ indicator
Authors: Tischbirek, Carsten and Birkner, Antje and Jia, Hongbo and Sakmann, Bert and Konnerth, Arthur
Journal: Proceedings of the National Academy of Sciences (2015): 11377--11382
Authors: Tischbirek, Carsten and Birkner, Antje and Jia, Hongbo and Sakmann, Bert and Konnerth, Arthur
Journal: Proceedings of the National Academy of Sciences (2015): 11377--11382
Application notes
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Buccutite™ Bioconjugation Technology
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?