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Fura-8FF™, pentapotassium salt

Fluorescence excitation spectra of Fura-8™ in the presence of 0 to 39 µM free Ca2+.
Fluorescence excitation spectra of Fura-8™ in the presence of 0 to 39 µM free Ca2+.
Fluorescence excitation spectra of Fura-8™ in the presence of 0 to 39 µM free Ca2+.
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Physical properties
Dissociation constant (Kd, nM)6000
Molecular weight837.97
SolventWater
Spectral properties
Excitation (nm)354
Emission (nm)524
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
837.97
Dissociation constant (Kd, nM)
6000
Excitation (nm)
354
Emission (nm)
524
The cell-impermeant Fura-8FF is an analog of Fura-8 with much lower calcium binding affinity, Kd ~10 µM. Fura-8FF has its emission shifted into longer visible wavelength that is compatible with the common filter sets. e., calculating the excitation intensity ratios at 354 nm and 415 nm by monitoring emission intensity at 530 nm.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Fura-8FF™, pentapotassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM119.336 µL596.68 µL1.193 mL5.967 mL11.934 mL
5 mM23.867 µL119.336 µL238.672 µL1.193 mL2.387 mL
10 mM11.934 µL59.668 µL119.336 µL596.68 µL1.193 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Excitation (nm)354
Emission (nm)524

Images


Citations


View all 2 citations: Citation Explorer
An essential role of NAD (P) H oxidase 2 in UVA-induced calcium oscillations in mast cells
Authors: Li, Zhi Ying and Jiang, Wen Yi and Cui, Zong Jie
Journal: Photochemical &amp; Photobiological Sciences (2015): 414--428
Lasting inhibition of receptor-mediated calcium oscillations in pancreatic acini by neutrophil respiratory burst--A novel mechanism for secretory blockade in acute pancreatitis?
Authors: Liang, Hui Yuan and Song, Zhi Min and Cui, Zong Jie
Journal: Biochemical and biophysical research communications (2013): 361--367

References


View all 119 references: Citation Explorer
Load of calcium probe Fura -2/AM in Escherichia coli cells
Authors: Shao M, Wang HM, Liu ZH, Shen P, Cai RX.
Journal: Wei Sheng Wu Xue Bao (2005): 805
An Excel-based model of Ca2+ diffusion and fura 2 measurements in a spherical cell
Authors: McHugh JM, Kenyon JL.
Journal: Am J Physiol Cell Physiol (2004): C342
Problems caused by high concentration of ATP on activation of the P2X7 receptor in bone marrow cells loaded with the Ca2+ fluorophore fura-2
Authors: Paredes-Gamero EJ, Franca JP, Moraes AA, Aguilar MO, Oshiro ME, Ferreira AT.
Journal: J Fluoresc (2004): 711
Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with fura-2
Authors: McConnell G, Riis E.
Journal: Phys Med Biol (2004): 4757
Abnormal spectra alteration observed in Triton calibration method for measuring [Ca2+]i with fluorescence indicator, fura-2
Authors: Xu T, Yang W, Huo XL, Song T.
Journal: J Biochem Biophys Methods (2004): 219
Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source
Authors: McConnell G, Smith GL, Girkin JM, Gurney AM, Ferguson AI.
Journal: Opt Lett (2003): 1742
AMPA-induced Ca(2+) influx in cultured rat cortical nonpyramidal neurones: pharmacological characterization using fura-2 microfluorimetry
Authors: Fischer W, Franke H, Scheibler P, Allgaier C, Illes P.
Journal: Eur J Pharmacol (2002): 53
Selective measurement of endothelial or smooth muscle [Ca(2+)](i) in pressurized/perfused cerebral arteries with fura-2
Authors: Marrelli SP., undefined
Journal: J Neurosci Methods (2000): 145
Excitation wavelengths for fura 2 provide a linear relationship between [Ca(2+)] and fluorescence ratio
Authors: Palmer BM, Moore RL.
Journal: Am J Physiol Cell Physiol (2000): C1278
Tyrosine kinase inhibitors and Ca2+ signaling: direct interactions with fura-2
Authors: Berts A, Minneman KP.
Journal: Eur J Pharmacol (2000): 35