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Coelenterazine *CAS#: 55779-48-1*

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Physical properties
Molecular weight423.46
SolventEthanol
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


CAS
55779-48-1
Molecular weight
423.46
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin's bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 µM to >100 µM. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10-20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.

Calculators


Common stock solution preparation

Table 1. Volume of Ethanol needed to reconstitute specific mass of Coelenterazine *CAS#: 55779-48-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM236.15 µL1.181 mL2.361 mL11.807 mL23.615 mL
5 mM47.23 µL236.15 µL472.3 µL2.361 mL4.723 mL
10 mM23.615 µL118.075 µL236.15 µL1.181 mL2.361 mL

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Citations


View all 3 citations: Citation Explorer
Intracellular Ca2+ is important for flagellin-triggered defense in Arabidopsis and involves inositol polyphosphate signaling
Authors: Ma, Yi and Zhao, Yichen and Berkowitz, Gerald A
Journal: Journal of Experimental Botany (2017)
Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells
Authors: Harikumar, Kaleeckal G and Yan, Yan and Xu, Ting-Hai and Melcher, Karsten and Xu, H Eric and Miller, Laurence J
Journal: (2017)
Orchestration of salivary secretion mediated by two different dopamine receptors in the blacklegged tick Ixodes scapularis
Authors: Kim, Donghun and Simo, Ladislav and Park, Yoonseong
Journal: Journal of Experimental Biology (2014): 3656--3663

References


View all 150 references: Citation Explorer
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Journal: Cell Signal (2006): 1664
Crystal structure of obelin after Ca2+-triggered bioluminescence suggests neutral coelenteramide as the primary excited state
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Journal: Proc Natl Acad Sci U S A (2006): 2570
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Self-illuminating quantum dots light the way
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Journal: Nat Biotechnol (2006): 326
Blue fluorescent protein from the calcium-sensitive photoprotein aequorin: catalytic properties for the oxidation of coelenterazine as an oxygenase
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Journal: FEBS Lett (2006): 1977
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