Coelenterazine cp *UltraPure grade*
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 415.48 |
Solvent | Ethanol |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
CAS 123437-25-2 | Molecular weight 415.48 |
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin's bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 µM to >100 µM. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10-20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
Calculators
Common stock solution preparation
Table 1. Volume of Ethanol needed to reconstitute specific mass of Coelenterazine cp *UltraPure grade* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 240.685 µL | 1.203 mL | 2.407 mL | 12.034 mL | 24.069 mL |
5 mM | 48.137 µL | 240.685 µL | 481.371 µL | 2.407 mL | 4.814 mL |
10 mM | 24.069 µL | 120.343 µL | 240.685 µL | 1.203 mL | 2.407 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Citations
View all 7 citations: Citation Explorer
Intracellular Ca2+ is important for flagellin-triggered defense in Arabidopsis and involves inositol polyphosphate signaling
Authors: Ma, Yi and Zhao, Yichen and Berkowitz, Gerald A
Journal: Journal of Experimental Botany (2017)
Authors: Ma, Yi and Zhao, Yichen and Berkowitz, Gerald A
Journal: Journal of Experimental Botany (2017)
Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells
Authors: Harikumar, Kaleeckal G and Yan, Yan and Xu, Ting-Hai and Melcher, Karsten and Xu, H Eric and Miller, Laurence J
Journal: (2017)
Authors: Harikumar, Kaleeckal G and Yan, Yan and Xu, Ting-Hai and Melcher, Karsten and Xu, H Eric and Miller, Laurence J
Journal: (2017)
The CLAVATA signaling pathway mediating stem cell fate in shoot meristems requires Ca2+ as a secondary cytosolic messenger
Authors: Chou, Hsuan and Zhu, Yingfang and Ma, Yi and Berkowitz, Gerald A
Journal: The Plant Journal (2016): 494--506
Authors: Chou, Hsuan and Zhu, Yingfang and Ma, Yi and Berkowitz, Gerald A
Journal: The Plant Journal (2016): 494--506
Orchestration of salivary secretion mediated by two different dopamine receptors in the blacklegged tick Ixodes scapularis
Authors: Kim, Donghun and Simo, Ladislav and Park, Yoonseong
Journal: Journal of Experimental Biology (2014): 3656--3663
Authors: Kim, Donghun and Simo, Ladislav and Park, Yoonseong
Journal: Journal of Experimental Biology (2014): 3656--3663
Teaching an old hormone new tricks: cytosolic Ca2+ elevation involvement in plant brassinosteroid signal transduction cascades
Authors: Zhao, Yichen and Qi, Zhi and Berkowitz, Gerald A
Journal: Plant Physiology (2013): 555--565
Authors: Zhao, Yichen and Qi, Zhi and Berkowitz, Gerald A
Journal: Plant Physiology (2013): 555--565
The Involvement of Secondary Messengers in Plant Receptor-Like Kinase Signal Transduction
Authors: Zhao, Yichen
Journal: (2013)
Authors: Zhao, Yichen
Journal: (2013)
References
View all 150 references: Citation Explorer
Luminescence of imidazo[1,2-a]pyrazin-3(7H)-one compounds
Authors: Teranishi K., undefined
Journal: Bioorg Chem. (2006)
Authors: Teranishi K., undefined
Journal: Bioorg Chem. (2006)
Extended bioluminescence resonance energy transfer (eBRET) for monitoring prolonged protein-protein interactions in live cells
Authors: Pfleger KD, Dromey JR, Dalrymple MB, Lim EM, Thomas WG, Eidne KA.
Journal: Cell Signal (2006): 1664
Authors: Pfleger KD, Dromey JR, Dalrymple MB, Lim EM, Thomas WG, Eidne KA.
Journal: Cell Signal (2006): 1664
Crystal structure of obelin after Ca2+-triggered bioluminescence suggests neutral coelenteramide as the primary excited state
Authors: Liu ZJ, Stepanyuk GA, Vysotski ES, Lee J, Markova SV, Malikova NP, Wang BC.
Journal: Proc Natl Acad Sci U S A (2006): 2570
Authors: Liu ZJ, Stepanyuk GA, Vysotski ES, Lee J, Markova SV, Malikova NP, Wang BC.
Journal: Proc Natl Acad Sci U S A (2006): 2570
In vivo testing of Renilla luciferase substrate analogs in an orthotopic murine model of human glioblastoma
Authors: Otto-Duessel M, Khankaldyyan V, Gonzalez-Gomez I, Jensen MC, Laug WE, Rosol M.
Journal: Mol Imaging (2006): 57
Authors: Otto-Duessel M, Khankaldyyan V, Gonzalez-Gomez I, Jensen MC, Laug WE, Rosol M.
Journal: Mol Imaging (2006): 57
Expression, purification and characterization of calcium-triggered luciferin-binding protein of Renilla reniformis
Authors: Inouye S., undefined
Journal: Protein Expr Purif. (2006)
Authors: Inouye S., undefined
Journal: Protein Expr Purif. (2006)
Transient expression of apoaequorin in zebrafish embryos: extending the ability to image calcium transients during later stages of development
Authors: Cheung CY, Webb SE, Meng A, Miller AL.
Journal: Int J Dev Biol (2006): 561
Authors: Cheung CY, Webb SE, Meng A, Miller AL.
Journal: Int J Dev Biol (2006): 561
Self-illuminating quantum dots light the way
Authors: Frangioni JV., undefined
Journal: Nat Biotechnol (2006): 326
Authors: Frangioni JV., undefined
Journal: Nat Biotechnol (2006): 326
Blue fluorescent protein from the calcium-sensitive photoprotein aequorin: catalytic properties for the oxidation of coelenterazine as an oxygenase
Authors: Inouye S, Sasaki S.
Journal: FEBS Lett (2006): 1977
Authors: Inouye S, Sasaki S.
Journal: FEBS Lett (2006): 1977
Mitochondrial adaptations within chronically ischemic swine myocardium
Authors: McFalls EO, Sluiter W, Schoonderwoerd K, Manintveld OC, Lamers JM, Bezstarosti K, van Beusekom HM, Sikora J, Ward HB, Merkus D, Duncker DJ.
Journal: J Mol Cell Cardiol (2006): 980
Authors: McFalls EO, Sluiter W, Schoonderwoerd K, Manintveld OC, Lamers JM, Bezstarosti K, van Beusekom HM, Sikora J, Ward HB, Merkus D, Duncker DJ.
Journal: J Mol Cell Cardiol (2006): 980
In vivo RNA interference-mediated ablation of MDR1 P-glycoprotein
Authors: Pichler A, Zelcer N, Prior JL, Kuil AJ, Piwnica-Worms D.
Journal: Clin Cancer Res (2005): 4487
Authors: Pichler A, Zelcer N, Prior JL, Kuil AJ, Piwnica-Worms D.
Journal: Clin Cancer Res (2005): 4487