Cl-NERF
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 451.86 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
See also: Intracellular pH
Molecular weight 451.86 |
Cl-NERF is a rhodol dye with pKa = 3.8. It has been mainly used for monitoring pH in acidic environments such as endosomes and lysosomes. Cl-NERF has a ratiometric excitation spectrum with 510/450 nm excitation ratio commonly used. The dextran conjugates of Cl-NERF has been used for detect the uptake of labeled probes into acidic organelles.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cl-NERF to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 221.307 µL | 1.107 mL | 2.213 mL | 11.065 mL | 22.131 mL |
5 mM | 44.261 µL | 221.307 µL | 442.615 µL | 2.213 mL | 4.426 mL |
10 mM | 22.131 µL | 110.654 µL | 221.307 µL | 1.107 mL | 2.213 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
NBD-Cl [4-Chloro-7-nitrobenzofurazan] *CAS 10199-89-0* | 467 | 539 | 22000 | - |
CL-APC [Cross linked-AlloPhycocyanin] | 651 | 660 | 730000 | 0.195 |
Images
References
View all 12 references: Citation Explorer
Inhibitors of acid secretion can benefit gastric wound repair independent of luminal pH effects on the site of damage
Authors: Demitrack ES, Aihara E, Kenny S, Varro A, Montrose MH.
Journal: Gut (2012): 804
Authors: Demitrack ES, Aihara E, Kenny S, Varro A, Montrose MH.
Journal: Gut (2012): 804
In vivo action of trefoil factor 2 (TFF2) to speed gastric repair is independent of cyclooxygenase
Authors: Xue L, Aihara E, Podolsky DK, Wang TC, Montrose MH.
Journal: Gut (2010): 1184
Authors: Xue L, Aihara E, Podolsky DK, Wang TC, Montrose MH.
Journal: Gut (2010): 1184
Damage to the gastric epithelium activates cellular bicarbonate secretion via SLC26A9 Cl(-)/HCO(3)(-)
Authors: Demitrack ES, Soleimani M, Montrose MH.
Journal: Am J Physiol Gastrointest Liver Physiol (2010): G255
Authors: Demitrack ES, Soleimani M, Montrose MH.
Journal: Am J Physiol Gastrointest Liver Physiol (2010): G255
Disruption of the Cox-1 gene slows repair of microscopic lesions in the mouse gastric epithelium
Authors: Starodub OT, Demitrack ES, Baumgartner HK, Montrose MH.
Journal: Am J Physiol Cell Physiol (2008): C223
Authors: Starodub OT, Demitrack ES, Baumgartner HK, Montrose MH.
Journal: Am J Physiol Cell Physiol (2008): C223
Regulated alkali secretion acts in tandem with unstirred layers to regulate mouse gastric surface pH
Authors: Baumgartner HK, Montrose MH.
Journal: Gastroenterology (2004): 774
Authors: Baumgartner HK, Montrose MH.
Journal: Gastroenterology (2004): 774
Cyclooxygenase 1 is required for pH control at the mouse gastric surface
Authors: Baumgartner HK, Starodub OT, Joehl JS, Tackett L, Montrose MH.
Journal: Gut (2004): 1751
Authors: Baumgartner HK, Starodub OT, Joehl JS, Tackett L, Montrose MH.
Journal: Gut (2004): 1751
Endogenous cyclo-oxygenase activity regulates mouse gastric surface pH
Authors: Baumgartner HK, Kirbiyik U, Coskun T, Chu S, Montrose MH.
Journal: J Physiol (2002): 871
Authors: Baumgartner HK, Kirbiyik U, Coskun T, Chu S, Montrose MH.
Journal: J Physiol (2002): 871
Intragastric pH regulates conversion from net acid to net alkaline secretion by the rat stomach
Authors: Coskun T, Chu S, Montrose MH.
Journal: Am J Physiol Gastrointest Liver Physiol (2001): G870
Authors: Coskun T, Chu S, Montrose MH.
Journal: Am J Physiol Gastrointest Liver Physiol (2001): G870
Lifetime-based pH sensors: indicators for acidic environments
Authors: Lin HJ, Szmacinski H, Lakowicz JR.
Journal: Anal Biochem (1999): 162
Authors: Lin HJ, Szmacinski H, Lakowicz JR.
Journal: Anal Biochem (1999): 162
Apoplastic pH in corn root gravitropism: a laser scanning confocal microscopy measurement
Authors: Taylor DP, Slattery J, Leopold AC.
Journal: Physiol Plant (1996): 35
Authors: Taylor DP, Slattery J, Leopold AC.
Journal: Physiol Plant (1996): 35
Application notes
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Cell Preparation Considerations and Troubleshooting
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FAQ
How to measure intracellular pH using flow cytometry?
I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?
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I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?
What is an AM ester?
What are the disadvantages of Nanodrop light spectrophotometer?
What are the advantages of a Nanodrop light spectrophotometer?
AssayWise
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RATIOWORKS pH 165, AM: A Dual Excitation/Emission Fluorescence Probe For Imaging Live Cells
Investigating Intercellular Channels: Focus on Gap Junctions
Nucleic Acid Detection, Quantification and Imaging
Cal-520® AM, a Development Overview