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Cl-NERF

Steady-state excitation and emission spectra of Cl-NERF in aqueous buffer solution with increasing pH moving from bottom to top at pH = 2.0 (red line), 4 (purple line), 5 (blue line) and 6 (green line).
Steady-state excitation and emission spectra of Cl-NERF in aqueous buffer solution with increasing pH moving from bottom to top at pH = 2.0 (red line), 4 (purple line), 5 (blue line) and 6 (green line).
Steady-state excitation and emission spectra of Cl-NERF in aqueous buffer solution with increasing pH moving from bottom to top at pH = 2.0 (red line), 4 (purple line), 5 (blue line) and 6 (green line).
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Physical properties
Molecular weight451.86
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
451.86
Cl-NERF is a rhodol dye with pKa = 3.8. It has been mainly used for monitoring pH in acidic environments such as endosomes and lysosomes. Cl-NERF has a ratiometric excitation spectrum with 510/450 nm excitation ratio commonly used. The dextran conjugates of Cl-NERF has been used for detect the uptake of labeled probes into acidic organelles.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cl-NERF to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM221.307 µL1.107 mL2.213 mL11.065 mL22.131 mL
5 mM44.261 µL221.307 µL442.615 µL2.213 mL4.426 mL
10 mM22.131 µL110.654 µL221.307 µL1.107 mL2.213 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
NBD-Cl [4-Chloro-7-nitrobenzofurazan] *CAS 10199-89-0*46753922000-
CL-APC [Cross linked-AlloPhycocyanin]6516607300000.195

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References


View all 12 references: Citation Explorer
Inhibitors of acid secretion can benefit gastric wound repair independent of luminal pH effects on the site of damage
Authors: Demitrack ES, Aihara E, Kenny S, Varro A, Montrose MH.
Journal: Gut (2012): 804
In vivo action of trefoil factor 2 (TFF2) to speed gastric repair is independent of cyclooxygenase
Authors: Xue L, Aihara E, Podolsky DK, Wang TC, Montrose MH.
Journal: Gut (2010): 1184
Damage to the gastric epithelium activates cellular bicarbonate secretion via SLC26A9 Cl(-)/HCO(3)(-)
Authors: Demitrack ES, Soleimani M, Montrose MH.
Journal: Am J Physiol Gastrointest Liver Physiol (2010): G255
Disruption of the Cox-1 gene slows repair of microscopic lesions in the mouse gastric epithelium
Authors: Starodub OT, Demitrack ES, Baumgartner HK, Montrose MH.
Journal: Am J Physiol Cell Physiol (2008): C223
Regulated alkali secretion acts in tandem with unstirred layers to regulate mouse gastric surface pH
Authors: Baumgartner HK, Montrose MH.
Journal: Gastroenterology (2004): 774
Cyclooxygenase 1 is required for pH control at the mouse gastric surface
Authors: Baumgartner HK, Starodub OT, Joehl JS, Tackett L, Montrose MH.
Journal: Gut (2004): 1751
Endogenous cyclo-oxygenase activity regulates mouse gastric surface pH
Authors: Baumgartner HK, Kirbiyik U, Coskun T, Chu S, Montrose MH.
Journal: J Physiol (2002): 871
Intragastric pH regulates conversion from net acid to net alkaline secretion by the rat stomach
Authors: Coskun T, Chu S, Montrose MH.
Journal: Am J Physiol Gastrointest Liver Physiol (2001): G870
Lifetime-based pH sensors: indicators for acidic environments
Authors: Lin HJ, Szmacinski H, Lakowicz JR.
Journal: Anal Biochem (1999): 162
Apoplastic pH in corn root gravitropism: a laser scanning confocal microscopy measurement
Authors: Taylor DP, Slattery J, Leopold AC.
Journal: Physiol Plant (1996): 35