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SPQ [6-Methoxy-N-(3-sulfopropyl)quinolinium]

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Physical properties
Molecular weight281.33
SolventDMSO
Spectral properties
Excitation (nm)317
Emission (nm)451
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


CAS
83907-40-8
Molecular weight
281.33
Excitation (nm)
317
Emission (nm)
451
A chloride indicator that has the fluorescence dependent of chloride concentration. Its fluorescence is quenched by chloride through collision quenching.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of SPQ [6-Methoxy-N-(3-sulfopropyl)quinolinium] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM355.454 µL1.777 mL3.555 mL17.773 mL35.545 mL
5 mM71.091 µL355.454 µL710.909 µL3.555 mL7.109 mL
10 mM35.545 µL177.727 µL355.454 µL1.777 mL3.555 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Excitation (nm)317
Emission (nm)451

Images


References


View all 71 references: Citation Explorer
Expression of band 3 protein on erythrocytes of malignant tumor patients and its impact on proliferation of K562 cells
Authors: Bai SZ, Liu XB, Xi YH, Wang TY, Fu GH.
Journal: Ai Zheng (2005): 543
Measurement of halide efflux from cultured and primary airway epithelial cells using fluorescence indicators
Authors: Munkonge F, Alton EW, Andersson C, Davidson H, Dragomir A, Edelman A, Farley R, Hjelte L, McLachlan G, Stern M, Roomans GM.
Journal: J Cyst Fibros (2004): 171
Halide fluxes in epithelial cells measured with an automated cell plate reader
Authors: Mahlangu DA, Dix JA.
Journal: Anal Biochem (2004): 28
Purinergic signaling underlies CFTR control of human airway epithelial cell volume
Authors: Braunstein GM, Zsembery A, Tucker TA, Schwiebert EM.
Journal: J Cyst Fibros (2004): 99
Normal function of the cystic fibrosis conductance regulator protein can be associated with homozygous (Delta)F508 mutation
Authors: Sermet-Gaudelus I, Vallee B, Urbin I, Torossi T, Marianovski R, Fajac A, Feuillet MN, Bresson JL, Lenoir G, Bernaudin JF, Edelman A.
Journal: Pediatr Res (2002): 628
The phorbol ester PMA and cyclic AMP activate different Cl(-) and HCO3(-) fluxes in C127 cells expressing CFTR
Authors: Zegarra-Moran O, Porcelli AM, Rugolo M.
Journal: Biochim Biophys Acta (2001): 120
Chloride-sensitive fluorescent indicators
Authors: Geddes CD, Apperson K, Karolin J, Birch DJ.
Journal: Anal Biochem (2001): 60
NO decreases thick ascending limb chloride absorption by reducing Na(+)-K(+)-2Cl(-) cotransporter activity
Authors: Ortiz PA, Hong NJ, Garvin JL.
Journal: Am J Physiol Renal Physiol (2001): F819
Evidence that systemic gentamicin suppresses premature stop mutations in patients with cystic fibrosis
Authors: Clancy JP, Bebok Z, Ruiz F, King C, Jones J, Walker L, Greer H, Hong J, Wing L, Macaluso M, Lyrene R, Sorscher EJ, Bedwell DM.
Journal: Am J Respir Crit Care Med (2001): 1683
Functional cystic fibrosis transmembrane conductance regulator tagged with an epitope of the vesicular stomatis virus glycoprotein can be addressed to the apical domain of polarized cells
Authors: Costa de Beauregard MA, Edelman A, Chesnoy-Marchais D, Tondelier D, Lapillonne A, El Marjou F, Robine S, Louvard D.
Journal: Eur J Cell Biol (2000): 795