Calculator
Oxonol V [Bis-(3-phenyl-5-oxoisoxazol-4-yl)pentamethine oxonol]
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 384.39 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
| Overview |  SDSProtocol |
CAS 61389-30-8 | Molecular weight 384.39 |
Oxonol V is a sensitive slow-response membrane potential probe that is widely used for measuring membrane potentials of many biological systems. The fluorescence of Oxonol V decreases upon membrane hyperpolarization. In general, slow-response probes exhibit potential-dependent changes in their transmembrane distribution that are accompanied by a fluorescence change. The magnitude of their optical responses is much larger than that of fast-response probes (typically a 1% fluorescence change per mV). Slow-response probes, which include cationic carbocyanines, rhodamines and anionic oxonols, are suitable for detecting changes in average membrane potentials of nonexcitable cells caused by respiratory activity, ion-channel permeability, drug binding and other factors.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Oxonol V [Bis-(3-phenyl-5-oxoisoxazol-4-yl)pentamethine oxonol] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 260.152 µL | 1.301 mL | 2.602 mL | 13.008 mL | 26.015 mL |
| 5 mM | 52.03 µL | 260.152 µL | 520.305 µL | 2.602 mL | 5.203 mL |
| 10 mM | 26.015 µL | 130.076 µL | 260.152 µL | 1.301 mL | 2.602 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Images
Figure 1. Chemical structure for Oxonol V [Bis-(3-phenyl-5-oxoisoxazol-4-yl)pentamethine oxonol]
References
View all 27 references: Citation Explorer
Use of an oxonol dye in combination with confocal laser scanning microscopy to monitor damage to Staphylococcus aureus cells during colonisation of silver-coated vascular grafts
Authors: Strathmann M, Wingender J.
Journal: Int J Antimicrob Agents (2004): 234
Authors: Strathmann M, Wingender J.
Journal: Int J Antimicrob Agents (2004): 234
Rapid assessment of antibiotic effects on Escherichia coli by bis-(1,3-dibutylbarbituric acid) trimethine oxonol and flow cytometry
Authors: Jepras RI, Paul FE, Pearson SC, Wilkinson MJ.
Journal: Antimicrob Agents Chemother (1997): 2001
Authors: Jepras RI, Paul FE, Pearson SC, Wilkinson MJ.
Journal: Antimicrob Agents Chemother (1997): 2001
Estimation of membrane potential deltapsi in reconstituted plasma membrane vesicles using a numerical model of oxonol VI distribution
Authors: Portele A, Lenz J, Hofer M.
Journal: J Bioenerg Biomembr (1997): 603
Authors: Portele A, Lenz J, Hofer M.
Journal: J Bioenerg Biomembr (1997): 603
Membrane potential changes visualized in complete growth media through confocal laser scanning microscopy of bis-oxonol-loaded cells
Authors: Dall'Asta V, Gatti R, Orl and ini G, Rossi PA, Rotoli BM, Sala R, Bussolati O, Gazzola GC.
Journal: Exp Cell Res (1997): 260
Authors: Dall'Asta V, Gatti R, Orl and ini G, Rossi PA, Rotoli BM, Sala R, Bussolati O, Gazzola GC.
Journal: Exp Cell Res (1997): 260
Muscarinic depolarization of SH-SY5Y human neuroblastoma cells as determined using oxonol V
Authors: Kukkonen JP, Hautala R, Akerman KE.
Journal: Neurosci Lett (1996): 57
Authors: Kukkonen JP, Hautala R, Akerman KE.
Journal: Neurosci Lett (1996): 57
An oxonol dye is the most potent known inhibitor of band 3-mediated anion exchange
Authors: Knauf PA, Law FY, Hahn K.
Journal: Am J Physiol (1995): C1073
Authors: Knauf PA, Law FY, Hahn K.
Journal: Am J Physiol (1995): C1073
Flow cytometric assessment of Escherichia coli and Salmonella typhimurium starvation-survival in seawater using rhodamine 123, propidium iodide, and oxonol
Authors: Lopez-Amoros R, Comas J, Vives-Rego J.
Journal: Appl Environ Microbiol (1995): 2521
Authors: Lopez-Amoros R, Comas J, Vives-Rego J.
Journal: Appl Environ Microbiol (1995): 2521
Volume-activated chloride channels in HL-60 cells: potent inhibition by an oxonol dye
Authors: Arreola J, Hallows KR, Knauf PA.
Journal: Am J Physiol (1995): C1063
Authors: Arreola J, Hallows KR, Knauf PA.
Journal: Am J Physiol (1995): C1063
Characterization of the steady-state and dynamic fluorescence properties of the potential-sensitive dye bis-(1,3-dibutylbarbituric acid)trimethine oxonol (Dibac4(3)) in model systems and cells
Authors: Epps DE, Wolfe ML, Groppi V.
Journal: Chem Phys Lipids (1994): 137
Authors: Epps DE, Wolfe ML, Groppi V.
Journal: Chem Phys Lipids (1994): 137
Anomalous response of oxonol-V to membrane potential in mitochondrial proton pumps
Authors: Ahmed I, Krishnamoorthy G.
Journal: Biochim Biophys Acta (1994): 131
Authors: Ahmed I, Krishnamoorthy G.
Journal: Biochim Biophys Acta (1994): 131