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Oxonol VI [Bis-(3-propyl-5-oxoisoxazol-4-yl)pentamethine oxonol]

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Physical properties
Molecular weight316.35
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


CAS
64724-75-0
Molecular weight
316.35
Oxonol VI is a sensitive slow-response membrane potential probe that has faster response than Oxonol V. The fluorescence of Oxonol VI decreases upon membrane hyperpolarization. In general, slow-response probes exhibit potential-dependent changes in their transmembrane distribution that are accompanied by a fluorescence change. The magnitude of their optical responses is much larger than that of fast-response probes (typically a 1% fluorescence change per mV). Slow-response probes, which include cationic carbocyanines, rhodamines and anionic oxonols, are suitable for detecting changes in average membrane potentials of nonexcitable cells caused by respiratory activity, ion-channel permeability, drug binding and other factors.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Oxonol VI [Bis-(3-propyl-5-oxoisoxazol-4-yl)pentamethine oxonol] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM316.106 µL1.581 mL3.161 mL15.805 mL31.611 mL
5 mM63.221 µL316.106 µL632.211 µL3.161 mL6.322 mL
10 mM31.611 µL158.053 µL316.106 µL1.581 mL3.161 mL

Molarity calculator

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References


View all 27 references: Citation Explorer
Use of an oxonol dye in combination with confocal laser scanning microscopy to monitor damage to Staphylococcus aureus cells during colonisation of silver-coated vascular grafts
Authors: Strathmann M, Wingender J.
Journal: Int J Antimicrob Agents (2004): 234
Rapid assessment of antibiotic effects on Escherichia coli by bis-(1,3-dibutylbarbituric acid) trimethine oxonol and flow cytometry
Authors: Jepras RI, Paul FE, Pearson SC, Wilkinson MJ.
Journal: Antimicrob Agents Chemother (1997): 2001
Estimation of membrane potential deltapsi in reconstituted plasma membrane vesicles using a numerical model of oxonol VI distribution
Authors: Portele A, Lenz J, Hofer M.
Journal: J Bioenerg Biomembr (1997): 603
Membrane potential changes visualized in complete growth media through confocal laser scanning microscopy of bis-oxonol-loaded cells
Authors: Dall'Asta V, Gatti R, Orl and ini G, Rossi PA, Rotoli BM, Sala R, Bussolati O, Gazzola GC.
Journal: Exp Cell Res (1997): 260
Muscarinic depolarization of SH-SY5Y human neuroblastoma cells as determined using oxonol V
Authors: Kukkonen JP, Hautala R, Akerman KE.
Journal: Neurosci Lett (1996): 57
An oxonol dye is the most potent known inhibitor of band 3-mediated anion exchange
Authors: Knauf PA, Law FY, Hahn K.
Journal: Am J Physiol (1995): C1073
Flow cytometric assessment of Escherichia coli and Salmonella typhimurium starvation-survival in seawater using rhodamine 123, propidium iodide, and oxonol
Authors: Lopez-Amoros R, Comas J, Vives-Rego J.
Journal: Appl Environ Microbiol (1995): 2521
Volume-activated chloride channels in HL-60 cells: potent inhibition by an oxonol dye
Authors: Arreola J, Hallows KR, Knauf PA.
Journal: Am J Physiol (1995): C1063
Characterization of the steady-state and dynamic fluorescence properties of the potential-sensitive dye bis-(1,3-dibutylbarbituric acid)trimethine oxonol (Dibac4(3)) in model systems and cells
Authors: Epps DE, Wolfe ML, Groppi V.
Journal: Chem Phys Lipids (1994): 137
Anomalous response of oxonol-V to membrane potential in mitochondrial proton pumps
Authors: Ahmed I, Krishnamoorthy G.
Journal: Biochim Biophys Acta (1994): 131